Product Info Summary
SKU: | A03320 |
---|---|
Size: | 100ug |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-Ajuba Antibody
SKU/Catalog Number
A03320
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-Ajuba Antibody (Catalog # A03320). Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-Ajuba Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03320)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 224-239 of Human Ajuba.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03320 is reactive to AJUBA in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Observed Molecular Weight
42 kDa
Calculated molecular weight
56934 MW
Background of Ajuba
Human AJUBA (also called JUB protein and ajuba homolog isoform 1) is a LIM domain protein suggested to bind and regulate the activity of Aurora A. Aurora A, which is involved in cell cycle regulation, is upregulated during mitosis, localizing to the centrosomes and microtubule regions proximal to the centrosomes.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03320 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:20,000 - 1:80,000
IP: 1:100
WB: 1:500 - 1:2,500
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Ajuba expression in 1) HeLa nuclear extract, and 2) HeLa, 3) A431, 4) Jurkat and 5) 293 whole cell lysates. Ajuba at 57KD was detected using rabbit anti-Ajuba Antigen Affinity purified polyclonal antibody (Catalog # A03320) at 1:500. Personal Communication. E. Pugacheva, Fox Chase Cancer Center, Phila, PA.
Click image to see more details
Western blot analysis of Ajuba expression in Ajuba-RFP fusion protein in cell lysates (arrow-head). Lanes correspond to 1) vector only trans-fection, 2) human Ajuba-RFP, 3) mouse Ajuba-RFP, and 4) mock transfection. Ajuba was detected using rabbit anti-Ajuba Antigen Affinity purified polyclonal antibody (Catalog # A03320) at 1:1700. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
Protein Target Info & Infographic
Gene/Protein Information For AJUBA (Source: Uniprot.org, NCBI)
Gene Name
AJUBA
Full Name
LIM domain-containing protein ajuba
Weight
56934 MW
Superfamily
zyxin/ajuba family
Alternative Names
Ajuba, Ajuba, JUB protein, ajuba homolog isoform 1, LIM domain-containing protein ajuba Ajuba|Jub|ajuba LIM protein|LIM domain-containing protein ajuba|protein ajuba
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AJUBA, check out the AJUBA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AJUBA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Ajuba Antibody (A03320)
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No publications found for A03320
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Customer Q&As
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5 Customer Q&As for Anti-Ajuba Antibody
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-Ajuba antibody A03320. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-02-26
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-26
Question
I see that the anti-Ajuba antibody A03320 works with IP, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-11-11
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-11-11
Question
We are currently using anti-Ajuba antibody A03320 for human tissue, and we are well pleased with the IP results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-22
Answer
The anti-Ajuba antibody (A03320) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-22
Question
Can you help my question with product A03320, anti-Ajuba antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-10-06
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A03320 anti-Ajuba antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-10-06
Question
Is a blocking peptide available for product anti-Ajuba antibody (A03320)?
M. Williams
Verified customer
Asked: 2016-03-09
Answer
We do provide the blocking peptide for product anti-Ajuba antibody (A03320). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2016-03-09