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Product Info Summary
| SKU: | A05117 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-Kelch-like protein 3 KLHL3 Antibody
View all Kelch-Like 3 Antibodies
SKU/Catalog Number
A05117
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Kelch-like protein 3 KLHL3 Antibody (Catalog # A05117). Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Kelch-like protein 3 KLHL3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05117)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from N-terminal of human KLHL3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A05117 is reactive to KLHL3 in Human, Mouse
Reconstitution
Observed Molecular Weight
111 kDa
Calculated molecular weight
64970 MW
Background of Kelch-Like 3
Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a regulator of ion transport in the distal nephron. The BCR(KLHL3) complex acts by mediating ubiquitination of WNK4, an inhibitor of potassium channel KCNJ1, leading to WNK4 degradation.
Lai F., Genomics 66:65-75(2000).
Hirosawa M., DNA Res. 6:329-336(1999).
Ota T., Nat. Genet. 36:40-45(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05117 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human brain tissue, using KLHL3 antibody A05117.
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Western blot analysis of extracts from RAW264.7 cells and 3T3 cells, using KLHL3 antibody A05117.
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Immunofluorescence analysis of A549 cells, using KLHL3 antibody A05117.
Protein Target Info & Infographic
Gene/Protein Information For KLHL3 (Source: Uniprot.org, NCBI)
Gene Name
KLHL3
Full Name
Kelch-like protein 3
Weight
64970 MW
Alternative Names
Kelch-like protein 3;KLHL3;KIAA1129; KLHL3 PHA2D kelch like family member 3 kelch-like protein 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KLHL3, check out the KLHL3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KLHL3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Kelch-like protein 3 KLHL3 Antibody (A05117)
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No publications found for A05117
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Customer Q&As
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5 Customer Q&As for Anti-Kelch-like protein 3 KLHL3 Antibody
Question
Does anti-KLHL3 antibody A05117 work for IF with bone marrow?
Verified Customer
Verified customer
Asked: 2019-12-30
Answer
According to the expression profile of bone marrow, KLHL3 is highly expressed in bone marrow. So, it is likely that anti-KLHL3 antibody A05117 will work for IF with bone marrow.
Boster Scientific Support
Answered: 2019-12-30
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for bone marrow using anti-KLHL3 antibody A05117. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-11-29
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-29
Question
We are currently using anti-KLHL3 antibody A05117 for mouse tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-03-28
Answer
The anti-KLHL3 antibody (A05117) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-03-28
Question
Is this A05117 anti-KLHL3 antibody reactive to the isotypes of KLHL3?
Verified Customer
Verified customer
Asked: 2018-07-24
Answer
The immunogen of A05117 anti-KLHL3 antibody is Synthesized peptide derived from N-terminal of human KLHL3. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-07-24
Question
My question regarding product A05117, anti-KLHL3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
G. Jones
Verified customer
Asked: 2018-06-13
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A05117 anti-KLHL3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-06-13
