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Product Info Summary
| SKU: | A00701 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Gli-2 Antibody
SKU/Catalog Number
A00701
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-Gli-2 Antibody (Catalog # A00701). Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-Gli-2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00701)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids from an internal region of Mouse Gli-2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00701 is reactive to GLI2 in Mouse
Reconstitution
Observed Molecular Weight
42 kDa
Calculated molecular weight
167.783kDa
Background of GLI-2
Gli-2 (also known as Zinc Finger Protein Gli-2, GLI-Kruppel family member GLI-2 or Tax helper protein) belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors that bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling, and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. Gli-2 is also thought to play a role during embryogenesis. The encoded protein is associated with several phenotypes: Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, pre-axial polydactyly type IV, and postaxial polydactyly types A1 and B. Anti-Gli 2 Antibody is useful for researchers interested in transcription factor activities, DNA binding, and chromatin binding research.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00701 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:15,000 - 1:60,000
IHC: 2 µg/ml to 20 µg/ml
WB: 1:500 - 1:2,000
Validation Images & Assay Conditions
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Affinity Purified anti-mouse Gli-2 antibody was used at 10
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Affinity Purified anti-mouse Gli-2 antibody was used at 10
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Figure 2. Western blot analysis of GLI2 using anti-GLI2 antibody (A00701).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GLI2 antigen affinity purified polyclonal antibody (Catalog # A00701) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Protein Target Info & Infographic
Gene/Protein Information For GLI2 (Source: Uniprot.org, NCBI)
Gene Name
GLI2
Full Name
Zinc finger protein GLI2
Weight
167.783kDa
Superfamily
GLI C2H2-type zinc-finger protein family
Alternative Names
Gli-2, Gli 2, Gli2, zinc finger protein GLI2, Tax helper protein, Thp GLI2 CJS, HPE9, PHS2, THP1, THP2 GLI family zinc finger 2 zinc finger protein GLI2|GLI family zinc finger protein 2|GLI-Kruppel family member GLI2|glioma-associated oncogene family zinc finger 2|oncogene GLI2|tax helper protein 1|tax helper protein 2|tax-responsive element-2 holding protein|tax-responsive element-25-bp sequence binding protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GLI2, check out the GLI2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GLI2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Gli-2 Antibody (A00701)
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