Product Info Summary
SKU: | A05560 |
---|---|
Size: | 100 µl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IF, IHC |
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Product info
Product Name
Anti-CST1/Cystatin Sn Antibody
View all Cystatin SN Antibodies
SKU/Catalog Number
A05560
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-CST1/Cystatin Sn Antibody (Catalog # A05560). Tested in IHC, IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CST1/Cystatin Sn Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05560)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human CST1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A05560 is reactive to CST1 in Human
Reconstitution
Observed Molecular Weight
111 kDa
Calculated molecular weight
16388 MW
Background of Cystatin SN
Human saliva appears to contain several cysteine proteinase inhibitors that are immunologically related to cystatin S but that differ in their specificity due to amino acid sequence differences. Cystatin SN, with a pI of 7.5, is a much better inhibitor of papain and dipeptidyl peptidase I than is cystatin S, although both inhibit ficin equally well.
Deloukas P., Nature 414:865-871(2001).
Zhang Z., Protein Sci. 13:2819-2824(2004).
Dickinson D.P., DNA Cell Biol. 21:47-65(2002).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05560 is guaranteed for IF, IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500
Validation Images & Assay Conditions
Click image to see more details
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using CST1 antibody A05560.
Click image to see more details
Immunofluorescence analysis of MCF-7 cells, using CST1 antibody A05560.
Protein Target Info & Infographic
Gene/Protein Information For CST1 (Source: Uniprot.org, NCBI)
Gene Name
CST1
Full Name
Cystatin-SN
Weight
16388 MW
Superfamily
cystatin family
Alternative Names
Cystatin-SN;Cystain-SA-I;Cystatin-1;Salivary cystatin-SA-1;CST1; CST1 cystatin SN cystatin-SN|cystain-SA-I|cystatin 1|cystatin SA-I|cysteine proteinase inhibitor, type 2 family|salivary cystatin-SA-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CST1, check out the CST1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CST1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CST1/Cystatin Sn Antibody (A05560)
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No publications found for A05560
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Customer Q&As
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4 Customer Q&As for Anti-CST1/Cystatin Sn Antibody
Question
Here is the WB image, lot number and protocol we used for saliva using anti-CST1/Cystatin Sn antibody A05560. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-04-30
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-30
Question
Does anti-CST1/Cystatin Sn antibody A05560 work on bovine IHC with saliva?
Verified Customer
Verified customer
Asked: 2020-01-16
Answer
Our lab technicians have not validated anti-CST1/Cystatin Sn antibody A05560 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-CST1/Cystatin Sn antibody A05560 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine saliva in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-16
Question
My question regards to test anti-CST1/Cystatin Sn antibody A05560 on human saliva for research purposes, then I may be interested in using anti-CST1/Cystatin Sn antibody A05560 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-10-02
Answer
The products we sell, including anti-CST1/Cystatin Sn antibody A05560, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-02
Question
Will anti-CST1/Cystatin Sn antibody A05560 work for IF with saliva?
Verified Customer
Verified customer
Asked: 2018-10-22
Answer
According to the expression profile of saliva, CST1 is highly expressed in saliva. So, it is likely that anti-CST1/Cystatin Sn antibody A05560 will work for IF with saliva.
Boster Scientific Support
Answered: 2018-10-22