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Product Info Summary
| SKU: | EK1654 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and urine. |
Product info
Product Name
Human Cystatin SN/CST1 ELISA Kit PicoKine®
View all Cystatin SN ELISA kits
SKU/Catalog Number
EK1654
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Cystatin SN/CST1 ELISA Kit PicoKine® (96 Tests). Quantitate Human CST1 in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Cystatin SN/CST1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1654)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: W21-S141
Sensitivity
<10 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1654 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1654 is reactive to CST1 in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and urine.
Application Guarantee
EK1654 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Cystatin SN
Cystatin-SN is a protein that in humans is encoded by the CST1 gene. The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes a cysteine proteinase inhibitor found in saliva, tears, urine, and seminal fluid.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1654-CAP | Anti-Human CST1 Pre-coated 96-well strip microplate | 1 |
| EK1654-ST | Human CST1 Standard | 2 vials, 10 ng/tube |
| EK1654-DA | Human CST1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.082 | 0.148 | 0.199 | 0.325 | 0.472 | 0.769 | 1.218 | 1.868 |
Data Example Images
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Human Cystatin SN/CST1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Cystatin SN, Dilution ratio of 1:10, concentration in serum and plasma is around 2 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Cystatin SN/CST1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 56 | 263 | 982 | 53 | 244 | 937 |
| Standard deviation | 4.08 | 12.36 | 54.01 | 4.66 | 15.86 | 57.15 |
| CV (%) | 7.3% | 4.7% | 5.5% | 8.8% | 6.5% | 6.1% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Cystatin SN in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 56 | 49 | 57 | 56 | 54 | 3.2 | 5.9% |
| Sample 2 | 263 | 286 | 255 | 285 | 272 | 13.55 | 4.9% |
| Sample 3 | 982 | 921 | 916 | 948 | 941 | 26.23 | 2.7% |
Protein Target Info & Infographic
Gene/Protein Information For CST1 (Source: Uniprot.Org, NCBI)
Gene Name
CST1
Full Name
Cystatin-SN
Weight
16.388kDa
Superfamily
cystatin family
Alternative Names
Cystatin-SN; Cystain-SA-I; Cystatin-1; Salivary cystatin-SA-1; CST1 CST1 cystatin SN cystatin-SN|cystain-SA-I|cystatin 1|cystatin SA-I|cysteine proteinase inhibitor, type 2 family|salivary cystatin-SA-1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CST1, check out the CST1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CST1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Cystatin SN/CST1 ELISA Kit PicoKine® (EK1654)
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No publications found for EK1654
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Customer Reviews
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Customer Q&As
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9 Customer Q&As for Human Cystatin SN/CST1 ELISA Kit PicoKine®
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
K. Roberts
Verified customer
Asked: 2020-08-27
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-08-27
Question
Q: can I use citrate plasma as samples in Human Cystatin SN Picokine® ELISA Kit (Catalog # EK1654)?
Verified Customer
Verified customer
Asked: 2020-07-20
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of Cystatin SN causing degradation of its protein structure. Cystatin SN may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the Cystatin SN ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-07-20
Question
Q: how to thaw whole blood sample for Cystatin SN ELISA after freezing?
Verified Customer
Verified customer
Asked: 2020-05-04
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Cystatin SN for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2020-05-04
Question
Q: how do I analyze ELISA data? I have obtained Cystatin SN level in urine.
Verified Customer
Verified customer
Asked: 2020-02-24
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2020-02-24
Question
What is EK1654 usually used for by others who bought this kit?
Verified customer
Asked: 2019-08-13
Answer
Our ELISA kits are usually used for evaluating either the presence of antigen or the presence of antibody in a sample, it is a useful tool for determining serum antibody concentrations. We do not have any information regarding the specific research customers who purchase this kit is doing as we could not find any publications with the Human Cystatin SN/CST1 ELISA Kit PicoKine™ (EK1654).
Boster Scientific Support
Answered: 2019-08-13
Question
Q: Can Cystatin SN ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2018-11-22
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is needed. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2018-11-22
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring Cystatin SN using the EK1654 Human Cystatin SN Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2017-10-01
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples in order to prevent protein degradation.
Boster Scientific Support
Answered: 2017-10-01
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2016-10-02
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2016-10-02
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
H. Yang
Verified customer
Asked: 2016-03-23
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2016-03-23
