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Product Info Summary
| SKU: | A12856-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-CRTH2 PTGDR2 Antibody
View all CRTH-2/GPR44 Antibodies
SKU/Catalog Number
A12856-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-CRTH2 PTGDR2 Antibody (Catalog # A12856-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
CRTH2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-CRTH2 PTGDR2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12856-1)
Host
Rabbit
Contents
CRTH2 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
CRTH2 antibody was raised against a 18 amino acid synthetic peptide from near the amino terminus of human CRTH2. The immunogen is located within the first 50 amino acids of CRTH2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least two different isoforms of CRTH2 are known to exist; this antibody will detect both isoforms.
Reactive Species
A12856-1 is reactive to PTGDR2 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
43268 MW
Background of CRTH-2/GPR44
The chemoattractant receptor-homologous molecule expressed on Th2 cells (CRTH2) is a recently identified receptor for the prostaglandin D2 (PGD2) in addition to the classic prostaglandin D receptor. CRTH2 is expressed on Th2 cells and eosinophils and mediates chemotaxis of these cells to PGD2 and is thus thought to be a key receptor mediating eosinophil and Th2 recruitment during allergic responses. However, CRTH2-null mice showed enhanced eosinophil recruitment into the lung consistent with observations that the CRTH2-null mice produced significantly higher amounts of interleukin-5 (IL-5) and IL-3. This suggests that CRTH2 plays a nonredundant role in restricting eosinophilia and allergic response in vivo.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A12856-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
CRTH2 antibody can be used for detection of CRTH2 by Western blot a 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of CRTH2 in Jurkat cell lysate with CRTH2 antibody at 1 μg/mL in (A) the absence and (B) presence of blocking peptide.
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Immunohistochemistry of CRTH2 in human heart tissue with CRTH2 antibody at 2.5 μg/mL.
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Immunofluorescence of CRTH2 in Human Heart tissue with CRTH2 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For PTGDR2 (Source: Uniprot.org, NCBI)
Gene Name
PTGDR2
Full Name
Prostaglandin D2 receptor 2
Weight
43268 MW
Superfamily
G-protein coupled receptor 1 family
Alternative Names
DP2, DL1R, CD294, CRTH2, GPR44, Prostaglandin D2 receptor 2, Chemoattractant receptor-homologous molecule expressed on Th2 cells PTGDR2 CD294, CRTH2, DL1R, DP2, GPR44 prostaglandin D2 receptor 2 prostaglandin D2 receptor 2|G-protein coupled receptor 44|chemoattractant receptor homologous molecule expressed on T helper type 2 cells|chemoattractant receptor-homologous molecule expressed on TH2 cells|putative G-protein coupled receptor 44
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PTGDR2, check out the PTGDR2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PTGDR2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CRTH2 PTGDR2 Antibody (A12856-1)
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