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Product Info Summary
| SKU: | A00289 |
|---|---|
| Size: | 100µg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Dopamine Receptor D2 Drd2 Antibody
View all Dopamine D2R/DRD2 Antibodies
SKU/Catalog Number
A00289
Size
100µg
Form
Liquid
Description
Boster Bio Anti-Dopamine Receptor D2 Drd2 Antibody (Catalog# A00289). Tested in ELISA, IHC, WB application(s). This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for long-term storage. Keep on ice at all times.
Cite This Product
Anti-Dopamine Receptor D2 Drd2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00289)
Host
Rabbit
Contents
Liquid. In PBS containing 50% glycerol and 0.01% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
Synthetic peptide corresponding to the sequence near the N-terminus of mouse dopamine receptor D2
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Predicted species reactivity based on epitope sequence homology: rhesus macaque, chimpanzee, horse, arctic fox, dog, opossum, ferret.
Reactive Species
A00289 is reactive to Drd2 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
152219 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00289 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Suitable for cell-based and membrane-based ELISA.
Predicted MW of ~51kDa.
Optimal conditions must be determined individually for each application.
Validation Images & Assay Conditions
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Cellular ELISA: HEK293 cells expressing dopamine receptor (D2) were treated with indicated concentrations of agonist (Quinpirol) and antagonist (Siperone) and probed with Dopamine Receptor (D2) pAb (1:1000 of a stock solution of 1μg/μL) by cellular ELISA.Data from vehicle treated cells were taken as 100%.Results are the mean ±SEM (n=3).
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Figure 1. Western blot analysis of Drd2 using anti-Drd2 antibody (A00289).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Drd2 antigen affinity purified polyclonal antibody (Catalog # A00289) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
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Figure 2. IHC analysis of Drd2 using anti-Drd2 antibody (A00289).
Drd2 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Drd2 Antibody (A00289) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For Drd2 (Source: Uniprot.org, NCBI)
Gene Name
Drd2
Full Name
D(2) dopamine receptor
Weight
152219 MW
Superfamily
G-protein coupled receptor 1 family
Alternative Names
Bromodomain-containing protein 4;Protein HUNK1;BRD4;HUNK1; DRD2 D2DR, D2R dopamine receptor D2 D(2) dopamine receptor|dopamine D2 receptor|seven transmembrane helix receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Drd2, check out the Drd2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Drd2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Dopamine Receptor D2 Drd2 Antibody (A00289)
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No publications found for A00289
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Customer Q&As
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7 Customer Q&As for Anti-Dopamine Receptor D2 Drd2 Antibody
Question
We are currently using anti-Dopamine Receptor D2 antibody A00289 for human tissue, and we are satisfied with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-10
Answer
The anti-Dopamine Receptor D2 antibody (A00289) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-10
Question
I see that the anti-Dopamine Receptor D2 antibody A00289 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-11-12
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-11-12
Question
I was wanting to use your anti-Dopamine Receptor D2 antibody for IHC for mouse adenohypophysis on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse adenohypophysis identification?
Verified Customer
Verified customer
Asked: 2019-08-15
Answer
You can see on the product datasheet, A00289 anti-Dopamine Receptor D2 antibody has been tested for ELISA, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse adenohypophysis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-15
Question
Is this A00289 anti-Dopamine Receptor D2 antibody reactive to the isotypes of DRD2?
B. Jones
Verified customer
Asked: 2019-08-08
Answer
The immunogen of A00289 anti-Dopamine Receptor D2 antibody is Synthetic peptide corresponding to the sequence near the N-terminus of mouse dopamine receptor D2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-08-08
Question
My question regarding product A00289, anti-Dopamine Receptor D2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-12-28
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00289 anti-Dopamine Receptor D2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-12-28
Question
Would anti-Dopamine Receptor D2 antibody A00289 work for IHC with adenohypophysis?
Verified Customer
Verified customer
Asked: 2017-10-04
Answer
According to the expression profile of adenohypophysis, DRD2 is highly expressed in adenohypophysis. So, it is likely that anti-Dopamine Receptor D2 antibody A00289 will work for IHC with adenohypophysis.
Boster Scientific Support
Answered: 2017-10-04
Question
Will anti-Dopamine Receptor D2 antibody A00289 work on bovine IHC with corpus striatum?
C. Krishna
Verified customer
Asked: 2017-08-01
Answer
Our lab technicians have not tested anti-Dopamine Receptor D2 antibody A00289 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-Dopamine Receptor D2 antibody A00289 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine corpus striatum in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-08-01
