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Product Info Summary
| SKU: | A00577-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Monkey, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-CREB1 Antibody Picoband®
SKU/Catalog Number
A00577-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CREB1 Antibody Picoband® catalog # A00577-1. Tested in WB, ELISA applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-CREB1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00577-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human CREB1 recombinant protein (Position: A7-R270). Human CREB1 shares 99.6% and 99.2% amino acid (aa) sequence identity with mouse and rat CREB1, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00577-1 is reactive to CREB1 in Human, Monkey, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
43,49 kDa
Calculated molecular weight
36,37,25 kDa
Background of CREB
CAMP responsive element binding protein 1, also known as CREB-1, is a protein that in humans is encoded by the CREB1 gene. This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00577-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Monkey, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human 293T whole cell, monkey COS-7 whole cell, rat brain tissue, mouse brain tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CREB1 using anti-CREB1 antibody (A00577-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: monkey COS-7 whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CREB1 antigen affinity purified polyclonal antibody (Catalog # A00577-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CREB1 at approximately 43,49 kDa. The expected band size for CREB1 is at 36,37,25 kDa.
Protein Target Info & Infographic
Gene/Protein Information For CREB1 (Source: Uniprot.org, NCBI)
Gene Name
CREB1
Full Name
Cyclic AMP-responsive element-binding protein 1
Weight
36,37,25 kDa
Superfamily
bZIP family
Alternative Names
active transcription factor CREB; cAMP responsive element binding protein 1; cAMP-response element-binding protein-1; cAMP-responsive element-binding protein 1; CREB; CREB1; CREB-1; cyclic AMP-responsive element-binding protein 1; MGC9284; pCREB; transactivator protein CREB1 CREB, CREB-1 cAMP responsive element binding protein 1 cyclic AMP-responsive element-binding protein 1|active transcription factor CREB|cAMP-response element-binding protein-1|cyclic adenosine 3,5-monophosphate response element binding protein|cyclic adenosine 3,5-monophosphate response element-binding protein CREB|transactivator protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CREB1, check out the CREB1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CREB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CREB1 Antibody Picoband® (A00577-1)
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