Product Info Summary
SKU: | A30467 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IF, IHC-P, ICC |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-CRBP III Antibody
SKU/Catalog Number
A30467
Size
100ul
Form
Liquid
Description
Boster Bio Anti-CRBP III Antibody (Catalog# A30467). Tested in IHC-P, IF, ICC, ELISA applications. This antibody reacts with Human, Rat, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CRBP III Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30467)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human CRBP III. Immunogen sequence location: 10-59
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A30467 is reactive to RBP5 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
72 kDa
Calculated molecular weight
15931 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30467 is guaranteed for ELISA, IF, IHC-P, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry, 1:100 - 1:300
Immunofluorescence, 1:200 - 1:1000
ELISA, 1:20000
Not yet tested in other applications.
Validation Images & Assay Conditions
Click image to see more details
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using CRBP III Antibody. The picture on the right is blocked with the synthesized peptide.
Click image to see more details
Immunofluorescence analysis of LOVO cells, using CRBP III Antibody. The picture on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For RBP5 (Source: Uniprot.org, NCBI)
Gene Name
RBP5
Full Name
Retinol-binding protein 5
Weight
15931 MW
Superfamily
calycin superfamily
Alternative Names
RBP5;Retinol-binding protein 5;Cellular retinol-binding protein III;CRBP-III;HRBPiso RBP5 CRBP-III, CRBP3, CRBPIII, HRBPiso retinol binding protein 5 retinol-binding protein 5|cellular retinol-binding protein III|putative cellular retinol-binding protein CRBP III|retinol binding protein 5, cellular
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RBP5, check out the RBP5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RBP5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CRBP III Antibody (A30467)
Hello CJ!
No publications found for A30467
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-CRBP III Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-CRBP III Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
7 Customer Q&As for Anti-CRBP III Antibody
Question
Is there a BSA free version of anti-CRBP III antibody A30467 available?
Verified Customer
Verified customer
Asked: 2019-11-18
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-CRBP III antibody A30467 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-11-18
Question
Can you help my question with product A30467, anti-CRBP III antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-08-15
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A30467 anti-CRBP III antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-15
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for adult mammalian kidney using anti-CRBP III antibody A30467. Let me know if you need anything else.
P. Anderson
Verified customer
Asked: 2019-05-23
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-23
Question
Does A30467 anti-CRBP III antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
J. Parker
Verified customer
Asked: 2018-06-25
Answer
You can see on the product datasheet, A30467 anti-CRBP III antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-06-25
Question
Is a blocking peptide available for product anti-CRBP III antibody (A30467)?
A. Wu
Verified customer
Asked: 2017-06-27
Answer
We do provide the blocking peptide for product anti-CRBP III antibody (A30467). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-06-27
Question
Does anti-CRBP III antibody A30467 work for IF with adult mammalian kidney?
T. Carter
Verified customer
Asked: 2016-09-01
Answer
According to the expression profile of adult mammalian kidney, RBP5 is highly expressed in adult mammalian kidney. So, it is likely that anti-CRBP III antibody A30467 will work for IF with adult mammalian kidney.
Boster Scientific Support
Answered: 2016-09-01
Question
We are currently using anti-CRBP III antibody A30467 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on primate tissues as well?
S. Mitchell
Verified customer
Asked: 2014-09-08
Answer
The anti-CRBP III antibody (A30467) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-09-08