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Product Info Summary
| SKU: | A05985 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-CPA1/Carboxypeptidase A1 Antibody
View all Carboxypeptidase A1/CPA1 Antibodies
SKU/Catalog Number
A05985
Size
100μl
Form
Liquid
Description
Boster Bio Anti-CPA1/Carboxypeptidase A1 Antibody catalog # A05985. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CPA1/Carboxypeptidase A1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05985)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human Carboxypeptidase A1. AA range:301-350
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A05985 is reactive to CPA1 in Human
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
47140 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05985 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of K562 cells using CPA1 Polyclonal antibody.
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Western Blot (WB) analysis of specific cells using CPA1 Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For CPA1 (Source: Uniprot.org, NCBI)
Gene Name
CPA1
Full Name
Carboxypeptidase A1
Weight
47140 MW
Superfamily
peptidase M14 family
Alternative Names
Carboxypeptidase A1;3.4.17.1;CPA1;CPA; CPA1 CPA carboxypeptidase A1 carboxypeptidase A1|carboxypeptidase A1 (pancreatic)|pancreatic carboxypeptidase A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CPA1, check out the CPA1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CPA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CPA1/Carboxypeptidase A1 Antibody (A05985)
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No publications found for A05985
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-CPA1/Carboxypeptidase A1 Antibody
Question
Do you have a BSA free version of anti-CPA1/Carboxypeptidase A1 antibody A05985 available?
Verified Customer
Verified customer
Asked: 2020-02-25
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-CPA1/Carboxypeptidase A1 antibody A05985 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-02-25
Question
Does A05985 anti-CPA1/Carboxypeptidase A1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-18
Answer
You can see on the product datasheet, A05985 anti-CPA1/Carboxypeptidase A1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-18
Question
Our lab want to know about to test anti-CPA1/Carboxypeptidase A1 antibody A05985 on human body of pancreas for research purposes, then I may be interested in using anti-CPA1/Carboxypeptidase A1 antibody A05985 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-09-16
Answer
The products we sell, including anti-CPA1/Carboxypeptidase A1 antibody A05985, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-16
Question
My question regarding product A05985, anti-CPA1/Carboxypeptidase A1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-24
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A05985 anti-CPA1/Carboxypeptidase A1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-24
Question
Would anti-CPA1/Carboxypeptidase A1 antibody A05985 work for WB with body of pancreas?
Verified Customer
Verified customer
Asked: 2018-10-26
Answer
According to the expression profile of body of pancreas, CPA1 is highly expressed in body of pancreas. So, it is likely that anti-CPA1/Carboxypeptidase A1 antibody A05985 will work for WB with body of pancreas.
Boster Scientific Support
Answered: 2018-10-26
Question
Is this A05985 anti-CPA1/Carboxypeptidase A1 antibody reactive to the isotypes of CPA1?
Z. Moore
Verified customer
Asked: 2013-09-20
Answer
The immunogen of A05985 anti-CPA1/Carboxypeptidase A1 antibody is Synthesized peptide derived from the Internal region of human CPA1. at AA rangle: 270-350. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-09-20
