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Product Info Summary
| SKU: | PA1747 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-IL2RA Antibody Picoband®
View all CD25/IL-2R alpha Antibodies
SKU/Catalog Number
PA1747
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IL2RA Antibody catalog # PA1747. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IL2RA Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1747)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human CD25/IL-2sR Alpha.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1747 is reactive to IL2RA in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
45 kDa
Calculated molecular weight
30819 MW
Background of CD25/IL-2R alpha
IL2RA (Interleukin 2 receptor, alpha), also called IL2R, IL2R, Alpha Chain or CD25, is a protein that in humans is encoded by the IL2RA gene.The IL2RA gene is mapped on 10p15.1. IL2RA is composed of strand-swapped "sushi-like" domains, unlike the classical cytokine receptor fold. As a result of this domain swap, IL2RA uses a composite surface to dock into a groove on IL2 that also serves as a binding site for antagonist drugs.Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Infection by the protozoan Trypanosoma cruzi causes Chagas disease, characterized by a reduction in the amount of IL2RA expressed on the surface of immune cells. This leads to chronic immune suppression, becoming increasingly severe over the course of many years and ultimately resulting in death if left untreated.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1747 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, By Heat
Positive Control
WB: human Jurkat whole cell lysates
IHC: human lymph cancer tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of IL2RA using anti-IL2RA antibody (PA1747).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant human CD25/IL2RA protein 10ng,
Lane 2: recombinant human CD25/IL2RA protein 5ng.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL2RA antigen affinity purified polyclonal antibody (Catalog # PA1747) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
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Figure 2. IHC analysis of IL2RA using anti-IL2RA antibody (PA1747).
IL2RA was detected in a paraffin-embedded section of human lymph cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IL2RA Antibody (PA1747) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. Western blot analysis of IL2RA using anti-IL2RA antibody (PA1747).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% milk for 2 hour at RT. The membrane was incubated with anti-IL2RA antibody (PA1747) at 1:1000 for 16 hours at 4℃ , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a mouse anti-rabbit secondary antibody at a dilution of 1:10,000 for 2 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL2RA at approximately 45 kDa. The expected band size for IL2RA is at 31 kDa.
Protein Target Info & Infographic
Gene/Protein Information For IL2RA (Source: Uniprot.org, NCBI)
Gene Name
IL2RA
Full Name
Interleukin-2 receptor subunit alpha
Weight
30819 MW
Alternative Names
Interleukin-2 receptor subunit alpha;IL-2 receptor subunit alpha;IL-2-RA;IL-2R subunit alpha;IL2-RA;TAC antigen;p55;CD25;IL2RA; IL2RA CD25, IDDM10, IL2R, IMD41, TCGFR, p55 interleukin 2 receptor subunit alpha interleukin-2 receptor subunit alpha|IL-2 receptor subunit alpha|IL-2R subunit alpha|TAC |interleukin 2 receptor, alpha
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IL2RA, check out the IL2RA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IL2RA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IL2RA Antibody Picoband® (PA1747)
Hello CJ!
PA1747 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Expression of a Functional IL-2 Receptor in Vascular Smooth Muscle Cells
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Customer Reviews
Have you used Anti-IL2RA Antibody Picoband®?
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1 Reviews For Anti-IL2RA Antibody Picoband®
0
Works everytime--Prakash A, NEUROSCIENCE, WRIGHT STATE UNIVERSITY, GRADUATE STUDENT
Excellent
Source: Biocompare.com
| Applications | Western Blot |
|---|---|
| Sample | Jurkat Lysates |
| Detection | ECL solution |
"Our lab is identifying IL-2 receptor components in non-lymphoid primary cell lines. We use Jurkat (E6-1) cell lines as a positive control for our experiments. Got one single band at the correct size. Very sensitive, can use at low concentration. Great antibody. Works well with whole cell lystes from our cell lines."
Prakash A
Verified customer
Submitted 2016-01-19
Customer Q&As
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Find answers in Q&As, reviews.
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9 Customer Q&As for Anti-IL2RA Antibody Picoband®
Question
do you know if our CD25 antibodies can detect the soluble form can it be used for coupling to Luminex beads
Verified Customer
Verified customer
Asked: 2020-02-21
Answer
PA1747 is able to detect the soluble form. It contains carrier and can not be used for coupling to Luminex beads.
Boster Scientific Support
Answered: 2020-02-21
Question
I see that the anti-IL2RA antibody PA1747 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-01-10
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-01-10
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lymph node using anti-IL2RA antibody PA1747. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-01-01
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-01
Question
Do you custom biotinylate antibodies? I am interested in purchasing biotinylated PA1747. What would be the production time for that and what would be the price?
Verified Customer
Verified customer
Asked: 2019-09-11
Answer
Except for PA1518, we can not conjugate current lots for all the other primary antibodies with Cat#PAXXXX. We can only manufacture a new lot for conjugation. The minimum ordering amount is 1mg. It takes four months to manufacture the new batch. The price for 1mg is $2400.
Boster Scientific Support
Answered: 2019-09-11
Question
See attached the WB image, lot number and protocol we used for lymph node using anti-IL2RA antibody PA1747. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-06-24
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-24
Question
Does PA1747 anti-IL2RA antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-05-22
Answer
As indicated on the product datasheet, PA1747 anti-IL2RA antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-05-22
Question
I am interested in to test anti-IL2RA antibody PA1747 on human lymph node for research purposes, then I may be interested in using anti-IL2RA antibody PA1747 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-03-26
Answer
The products we sell, including anti-IL2RA antibody PA1747, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-03-26
Question
We are currently using anti-IL2RA antibody PA1747 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on dog tissues as well?
N. Parker
Verified customer
Asked: 2015-04-06
Answer
The anti-IL2RA antibody (PA1747) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-04-06
Question
Is a blocking peptide available for product anti-IL2RA antibody (PA1747)?
J. Zhao
Verified customer
Asked: 2014-09-25
Answer
We do provide the blocking peptide for product anti-IL2RA antibody (PA1747). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2014-09-25
