Anti-COX6B1 Antibody (N-term)

Product Name

Anti-COX6B1 Antibody (N-term)

View all COX6B1 Antibodies

SKU/Catalog Number

A10285-1

Size

80 µl

Description

Boster Bio Anti-COX6B1 Antibody (N-term) (Catalog # A10285-1). Tested in IF, WB application(s). This antibody reacts with Human.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-COX6B1 Antibody (N-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10285-1)

Host

Rabbit

Contents

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

This COX6B1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 8-22 amino acids from the N-terminal region of human COX6B1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A10285-1 is reactive to COX6B1 in Human

Applications

A10285-1 is guaranteed for IF, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

10192 Da

Background of COX6B1

Connects the two COX monomers into the physiological dimeric form (By similarity).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

IF: 1:25
WB: 1:1000

Validation Images & Assay Conditions

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Fluorescent image of A549 cells stained with COX6B1 Antibody (N-term). A10285-1 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).

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Western blot analysis of lysates from A549, Hela, HepG2, HL-60 cell line and human liver tissue lysate(from left to right), using COX6B1 Antibody (N-term). A10285-1 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

Gene/Protein Information For COX6B1 (Source: Uniprot.org, NCBI)

Alternative Names

COX VIb-1; COX6B; COXG; COXVIb1; cytochrome c oxidase subunit 6B1; Cytochrome c oxidase subunit VIb isoform 1; cytochrome c oxidase subunit VIb polypeptide 1 (ubiquitous); cytochrome c oxidase subunit Vib COX6B1 COX6B, COXG, COXVIb1, MC4DN7 cytochrome c oxidase subunit 6B1 cytochrome c oxidase subunit 6B1|COX VIb-1|cytochrome c oxidase subunit VIb polypeptide 1 (ubiquitous)

For more info on COX6B1, check out the COX6B1 Infographic

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In this infographic, you will see the following information for COX6B1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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