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Product Info Summary
| SKU: | A10078-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-COX4I2 Antibody Picoband®
SKU/Catalog Number
A10078-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-COX4I2 Antibody Picoband® catalog # A10078-2. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-COX4I2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10078-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human COX4I2, which shares 88.2% and 82.4% amino acid (aa) sequence identity with mouse and rat COX4I2, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
A10078-2 is reactive to COX4I2 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
20 kDa
Calculated molecular weight
41409 MW
Background of Cox4i2
Cytochrome c oxidase subunit 4 isoform 2, mitochondrial is an enzyme that in humans is encoded by the COX4I2 gene. Cytochrome c oxidase (COX), the terminal enzyme of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. It is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may be involved in the regulation and assembly of the complex. This nuclear gene encodes isoform 2 of subunit IV. Isoform 1 of subunit IV is encoded by a different gene, however, the two genes show a similar structural organization. Subunit IV is the largest nuclear encoded subunit which plays a pivotal role in COX regulation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10078-2 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Positive Control
WB: human HepG2 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of COX4I2 using anti-COX4I2 antibody (A10078-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COX4I2 antigen affinity purified polyclonal antibody (Catalog # A10078-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for COX4I2 at approximately 20 kDa. The expected band size for COX4I2 is at 20 kDa.
Protein Target Info & Infographic
Gene/Protein Information For COX4I2 (Source: Uniprot.org, NCBI)
Gene Name
COX4I2
Full Name
Cytochrome c oxidase subunit 4 isoform 2, mitochondrial
Weight
41409 MW
Superfamily
cytochrome c oxidase IV family
Alternative Names
Protein NDRG3;N-myc downstream-regulated gene 3 protein;NDRG3; Cox4i2|Cox4, Cox4b, CoxIV-2|cytochrome c oxidase subunit 4I2|cytochrome c oxidase subunit 4 isoform 2, mitochondrial|cytochrome c oxidase, subunit IVb
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on COX4I2, check out the COX4I2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for COX4I2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-COX4I2 Antibody Picoband® (A10078-2)
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