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Product Info Summary
| SKU: | A02443-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-COMP Antibody Picoband®
View all COMP/Thrombospondin-5 Antibodies
SKU/Catalog Number
A02443-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-COMP Antibody Picoband® catalog # A02443-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-COMP Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02443-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human COMP recombinant protein (Position: Q505-A757).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02443-1 is reactive to COMP in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
110 kDa
Calculated molecular weight
82,860 MW
Background of COMP/Thrombospondin-5
COMP is a pentameric extracellular matrix protein that catalyzes the assembly of collagens and promotes formation of well-defined fibrils. This gene is mapped to 19q13.32. The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02443-1 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human HepG2 whole cell, human Hacat whole cell, human CACO-2 whole cell, human 293T whole cell, rat cartilage tissue, mouse cartilage tissue
IHC: mouse knee-joint tissue, rat knee-joint tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of COMP using anti-COMP antibody (A02443-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human Hacat whole cell lysates,
Lane 3: human CACO-2 whole cell lysates,
Lane 4: human 293T whole cell lysates,
Lane 5: rat cartilage tissue lysates,
Lane 6: mouse cartilage tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COMP antigen affinity purified polyclonal antibody (Catalog # A02443-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for COMP at approximately 110 kDa. The expected band size for COMP is at 83 kDa.
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Figure 2. IHC analysis of COMP using anti-COMP antibody (A02443-1).
COMP was detected in a paraffin-embedded section of mouse knee-joint tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-COMP Antibody (A02443-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of COMP using anti-COMP antibody (A02443-1).
COMP was detected in a paraffin-embedded section of rat knee-joint tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-COMP Antibody (A02443-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For COMP (Source: Uniprot.org, NCBI)
Gene Name
COMP
Full Name
Cartilage oligomeric matrix protein
Weight
82,860 MW
Superfamily
thrombospondin family
Alternative Names
Cartilage oligomeric matrix protein; COMP; Thrombospondin-5; TSP5; COMP COMP CTS2, EDM1, EPD1, MED, PSACH, THBS5, TSP5 cartilage oligomeric matrix protein cartilage oligomeric matrix protein|cartilage oligomeric matrix protein (pseudoachondroplasia, epiphyseal dysplasia 1, multiple)|pseudoachondroplasia (epiphyseal dysplasia 1, multiple)|thrombospondin-5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on COMP, check out the COMP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for COMP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-COMP Antibody Picoband® (A02443-1)
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Customer Q&As
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16 Customer Q&As for Anti-COMP Antibody Picoband®
Question
I see that the anti-COMP antibody A02443-1 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-03-24
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-03-24
Question
Is a blocking peptide available for product anti-COMP antibody (A02443-1)?
Verified Customer
Verified customer
Asked: 2020-03-05
Answer
We do provide the blocking peptide for product anti-COMP antibody (A02443-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-03-05
Question
Our team were satisfied with the WB result of your anti-COMP antibody. However we have observed positive staining in ovary secreted using this antibody. Is that expected? Could you tell me where is COMP supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-02-11
Answer
From literature, ovary does express COMP. Generally COMP expresses in secreted, extracellular space, extracellular. Regarding which tissues have COMP expression, here are a few articles citing expression in various tissues:
Cartilage, Pubmed ID: 7713493
Liver, Pubmed ID: 24275569
Ovary, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-02-11
Question
We have tried in the past anti-COMP antibody for WB on tendon of biceps brachii last year. I am using mouse, and We want to use the antibody for ICC next. We are interested in examining tendon of biceps brachii as well as cartilage in our next experiment. Could give a recommendation on which antibody would work the best for ICC?
Verified Customer
Verified customer
Asked: 2019-12-03
Answer
I took a look at the website and datasheets of our anti-COMP antibody and I see that A02443-1 has been validated on mouse in both WB and ICC. Thus A02443-1 should work for your application. Our Boster satisfaction guarantee will cover this product for ICC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for ICC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-12-03
Question
Does A02443-1 anti-COMP antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-11-26
Answer
It shows on the product datasheet, A02443-1 anti-COMP antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-26
Question
you antibody to test anti-COMP antibody A02443-1 on rat tendon of biceps brachii for research purposes, then I may be interested in using anti-COMP antibody A02443-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-10-25
Answer
The products we sell, including anti-COMP antibody A02443-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-25
Question
Does anti-COMP antibody A02443-1 work for IF with tendon of biceps brachii?
Verified Customer
Verified customer
Asked: 2019-04-29
Answer
According to the expression profile of tendon of biceps brachii, COMP is highly expressed in tendon of biceps brachii. So, it is likely that anti-COMP antibody A02443-1 will work for IF with tendon of biceps brachii.
Boster Scientific Support
Answered: 2019-04-29
Question
We have been able to see staining in rat tendon of biceps brachii. Are there any suggestions? Is anti-COMP antibody supposed to stain tendon of biceps brachii positively?
Verified Customer
Verified customer
Asked: 2018-10-05
Answer
From what I have seen in literature tendon of biceps brachii does express COMP. From what I have seen in Uniprot.org, COMP is expressed in tendon of biceps brachii, cartilage, ovary, liver, among other tissues. Regarding which tissues have COMP expression, here are a few articles citing expression in various tissues:
Cartilage, Pubmed ID: 7713493
Liver, Pubmed ID: 24275569
Ovary, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-10-05
Question
Here is the WB image, lot number and protocol we used for tendon of biceps brachii using anti-COMP antibody A02443-1. Please let me know if you require anything else.
L. Miller
Verified customer
Asked: 2018-08-23
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-08-23
Question
I was wanting to use your anti-COMP antibody for IF for rat tendon of biceps brachii on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat tendon of biceps brachii identification?
Verified Customer
Verified customer
Asked: 2018-02-13
Answer
It shows on the product datasheet, A02443-1 anti-COMP antibody has been validated for ELISA, Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat tendon of biceps brachii in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-02-13
Question
I would like using your anti-COMP antibody for collagen fibril organization studies. Has this antibody been tested with western blotting on ovary cancer tissue? We would like to see some validation images before ordering.
F. Parker
Verified customer
Asked: 2018-02-08
Answer
Thank you for your inquiry. This A02443-1 anti-COMP antibody is tested on human cholangiocarcinoma tissue, placenta tissue, gastric cancer tissue, intestinal cancer tissue, squama cancer tissue, ovary cancer tissue, hepg2 cells. It is guaranteed to work for ELISA, Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-02-08
Question
Is this A02443-1 anti-COMP antibody reactive to the isotypes of COMP?
Verified Customer
Verified customer
Asked: 2017-09-06
Answer
The immunogen of A02443-1 anti-COMP antibody is E. coli-derived human COMP recombinant protein (Position: Q505-A757). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-09-06
Question
Do you have a BSA free version of anti-COMP antibody A02443-1 available?
Z. Li
Verified customer
Asked: 2017-01-09
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-COMP antibody A02443-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-01-09
Question
We are currently using anti-COMP antibody A02443-1 for rat tissue, and we are content with the Flow Cytometry results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on zebrafish tissues as well?
T. Carter
Verified customer
Asked: 2016-11-09
Answer
The anti-COMP antibody (A02443-1) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-11-09
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for tendon of biceps brachii using anti-COMP antibody A02443-1. Let me know if you need anything else.
B. Bhatt
Verified customer
Asked: 2016-09-20
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-09-20
Question
My question regarding product A02443-1, anti-COMP antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
A. Huang
Verified customer
Asked: 2013-06-20
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A02443-1 anti-COMP antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-06-20
