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Product Info Summary
| SKU: | A10530 |
|---|---|
| Size: | 100uL |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, WB |
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Product info
Product Name
Anti-CAND2 Antibody
SKU/Catalog Number
A10530
Size
100uL
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-CAND2 Antibody (Catalog # A10530). Tested in IP, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store CAND2 antibody at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-CAND2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10530)
Host
Rabbit
Contents
0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
Antiserum
Immunogen
This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 1130-1143 of Human CAND2/TIP120B (C-terminal) coupled to KLH.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A10530 is reactive to CAND2 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
63622 MW
Background of TIP120B
Anti-CAND2 antibody is ideal for western blotting, ELISA and IHC. CAND2 is also known as TIP120B, and TATA-binding protein-interacting protein 120B. While both CAND1 (TIP120A) and CAND2 (TIP120B) are TATA-binding proteins and form complexes with various nuclear proteins involved in the control of eukaryotic gene transcription, CAND2 (TIP120B) is expressed specifically in muscle and heart tissue. This is contrary to the ubiquitous expression of CAND1 (TIP120A). TIP120 homologs exist in various higher eukaryotes including D. melanogaster, C. elegans, and A. thaliana. TIP120B is 60% identical in amino acid sequence to TIP120A.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10530 is guaranteed for IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:2,000 - 1:10,000
IHC: User optimized
IP: User optimized
WB: 1:500 - 1:1,000
Validation Images & Assay Conditions
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Most modifiers mature by proteolytic processing from inactive precursors (a; amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein) /CBC(cullin-2/elongin B/elonginC) -like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin.Data contributed by S.Jentsch.
Protein Target Info & Infographic
Gene/Protein Information For CAND2 (Source: Uniprot.org, NCBI)
Gene Name
CAND2
Full Name
Cullin-associated NEDD8-dissociated protein 2
Weight
63622 MW
Superfamily
CAND family
Alternative Names
CAND2, Cullin associated NEDD8 dissociated protein 2, p120 CAND2, TATA binding protein interacting protein 120B, TBP interacting protein, Tp120b CAND2 TIP120B, Tp120b cullin associated and neddylation dissociated 2 (putative) cullin-associated NEDD8-dissociated protein 2|TBP interacting protein|TBP-interacting protein 120B|TBP-interacting protein of 120 kDa B|cullin-associated and neddylation-dissociated protein 2|epididymis secretory sperm binding protein|epididymis tissue protein Li 169|p120 CAND2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CAND2, check out the CAND2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CAND2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CAND2 Antibody (A10530)
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No publications found for A10530
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Customer Q&As
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3 Customer Q&As for Anti-CAND2 Antibody
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for skeletal muscle tissue of rectus abdominis using anti-CAND2 antibody A10530. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-10-23
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-23
Question
We are currently using anti-CAND2 antibody A10530 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-02
Answer
The anti-CAND2 antibody (A10530) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-02
Question
I see that the anti-CAND2 antibody A10530 works with WB, what is the protocol used to produce the result images on the product page?
C. Jones
Verified customer
Asked: 2013-05-24
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2013-05-24
