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Product Info Summary
| SKU: | A00590-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-Clusterin-Alpha (E436) Antibody
View all Clusterin/APOJ Antibodies
SKU/Catalog Number
A00590-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Clusterin-Alpha (E436) Antibody catalog # A00590-1. Tested in WB,IHC,IF applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Clusterin-Alpha (E436) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00590-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic peptide, corresponding to amino acids C-terminus of Human Clusterin-α.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00590-1 is reactive to CLU in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
52495 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00590-1 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
IF: 1:50-1:200
Validation Images & Assay Conditions
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Figure 2. Immunohistochemistry validation of CLU using Anti-Clusterin-Alpha (E436) Antibody (A00590-1).
Immunohistochemistry (IHC) analyzes of Clusterin-α (E436) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50.showing cytoplasmic and nucleus /cell membrane staining. Negative control (the right)Using PBS instead of primary antibody
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Figure 1. Western blotting validation for Anti-Clusterin-Alpha (E436) Antibody A00590-1
Western blot (WB) analysis of Clusterin-α (E436) pAb at 1:500 dilutionLane1:Hela whole cell lysate(40ug)
Lane2:A549 whole cell lysate(40ug)
Lane3:SGC7901 whole cell lysate(40ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For CLU (Source: Uniprot.org, NCBI)
Gene Name
CLU
Full Name
Clusterin
Weight
52495 MW
Superfamily
clusterin family
Alternative Names
Clusterin;Aging-associated gene 4 protein;Apolipoprotein J;Apo-J;Complement cytolysis inhibitor;CLI;Complement-associated protein SP-40,40;Ku70-binding protein 1;NA1/NA2;Testosterone-repressed prostate message 2;TRPM-2;Clusterin beta chain;ApoJalpha;Complement cytolysis inhibitor a chain;Clusterin alpha chain;ApoJbeta;Complement cytolysis inhibitor b chain;CLU;APOJ, CLI, KUB1;AAG4; CLU AAG4, APO-J, APOJ, CLI1, CLU2, KUB1, NA1/NA2, SGP-2, SGP2, SP-40, TRPM-2, TRPM2, CLU clusterin clusterin|aging-associated protein 4|apolipoprotein J|complement cytolysis inhibitor|complement lysis inhibitor|complement-associated protein SP-40,40|epididymis secretory sperm binding protein|ku70-binding protein 1|sulfated glycoprotein 2|testosterone-repressed prostate message 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CLU, check out the CLU Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CLU: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Clusterin-Alpha (E436) Antibody (A00590-1)
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