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Product Info Summary
| SKU: | A07404 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-Cell death activator CIDE-B CIDEB Antibody
SKU/Catalog Number
A07404
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Cell death activator CIDE-B CIDEB Antibody (Catalog # A07404). Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cell death activator CIDE-B CIDEB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07404)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human CIDEB.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A07404 is reactive to CIDEB in Human, Mouse
Reconstitution
Calculated molecular weight
24678 MW
Background of Cideb
Activates apoptosis.
Lugovskoy A.A., Cell 99:747-755(1999).
Inohara N., EMBO J. 17:2526-2533(1998).
Ota T., Nat. Genet. 36:40-45(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07404 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human cervix carcinoma tissue using CIDEB antibody A07404.
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Western blot analysis of extracts from RAW264.7 cells and HT-29 cells, using CIDEB antibody A07404.
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Immunofluorescence analysis of COS-7 cells, using CIDEB antibody A07404.
Protein Target Info & Infographic
Gene/Protein Information For CIDEB (Source: Uniprot.org, NCBI)
Gene Name
CIDEB
Full Name
Cell death activator CIDE-B
Weight
24678 MW
Alternative Names
Cell death activator CIDE-B;Cell death-inducing DFFA-like effector B;CIDEB; Cideb|1110030C18Rik, AI790179, CIDE-, CIDE-B|cell death-inducing DNA fragmentation factor, alpha subunit-like effector B|cell death activator CIDE-B|DFFA-like B|cell death-inducing DFFA-like effector B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CIDEB, check out the CIDEB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CIDEB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cell death activator CIDE-B CIDEB Antibody (A07404)
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No publications found for A07404
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Customer Q&As
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7 Customer Q&As for Anti-Cell death activator CIDE-B CIDEB Antibody
Question
We are currently using anti-CIDEB antibody A07404 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on feline tissues as well?
B. Wu
Verified customer
Asked: 2019-12-23
Answer
The anti-CIDEB antibody (A07404) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-23
Question
See attached the WB image, lot number and protocol we used for ileal mucosa using anti-CIDEB antibody A07404. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-11-28
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-28
Question
My question regarding product A07404, anti-CIDEB antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-19
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A07404 anti-CIDEB antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-19
Question
Does anti-CIDEB antibody A07404 work on bovine WB with liver?
Verified Customer
Verified customer
Asked: 2019-08-12
Answer
Our lab technicians have not validated anti-CIDEB antibody A07404 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-CIDEB antibody A07404 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-12
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for ileal mucosa using anti-CIDEB antibody A07404. Let me know if you need anything else.
P. Banerjee
Verified customer
Asked: 2018-07-02
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-07-02
Question
Is a blocking peptide available for product anti-CIDEB antibody (A07404)?
E. Zhao
Verified customer
Asked: 2014-04-18
Answer
We do provide the blocking peptide for product anti-CIDEB antibody (A07404). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2014-04-18
Question
Would A07404 anti-CIDEB antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
F. Johnson
Verified customer
Asked: 2013-12-11
Answer
As indicated on the product datasheet, A07404 anti-CIDEB antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-12-11
