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Product Info Summary
| SKU: | A07404-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-CIDE-B Antibody
SKU/Catalog Number
A07404-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-CIDE-B Antibody (Catalog # A07404-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
CIDE-B antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-CIDE-B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07404-1)
Host
Rabbit
Contents
CIDE-B Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
CIDE-B antibody was raised against a peptide corresponding to 15 amino acids near the carboxy terminus of human CIDE-B. The immunogen is located within the last 50 amino acids of CIDE-B.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
CIDE-B antibody has no cross activity to CIDE-A.
Reactive Species
A07404-1 is reactive to CIDEB in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
24678 MW
Background of Cideb
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. DFF45/ICAD has been identified as inhibitor of caspase activated DNase DFF40/CAD. DFF45 related proteins CIDE-A and CIDE-B (for cell death-inducing DFF-like effector A and B) were recently identified. CIDE contains a new type of domain termed CIDE-N, which has high homology with the regulatory domains of DFF45/ICAD and DFF40/CAD. Expression of CIDE-B induces apoptosis, which is inhibited by DFF45. CIDE-B is a DFF45-inhibitable effector that promotes cell death and DNA fragmentation. CIDE-B is expressed mainly in liver and at lower levels in spleen, kidney, peripheral blood lymphocytes and bone marrow.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07404-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
CIDE-B antibody can be used for detection of CIDE-B by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of CIDE-B in mouse small intestine tissue lysate with CIDE-B antibody at (A) 0.5
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Immunohistochemistry of CIDE-B in human small intestine tissue with CIDE-B antibody at 5 μg/mL.
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Immunofluorescence of CIDE-B in Human Small Intestine cells with CIDE-B antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For CIDEB (Source: Uniprot.org, NCBI)
Gene Name
CIDEB
Full Name
Cell death activator CIDE-B
Weight
24678 MW
Alternative Names
CIDE-B, AI790179, 1110030C18Rik, Cell death activator CIDE-B, Cell death-inducing DFFA-like effector B Cideb|1110030C18Rik, AI790179, CIDE-, CIDE-B|cell death-inducing DNA fragmentation factor, alpha subunit-like effector B|cell death activator CIDE-B|DFFA-like B|cell death-inducing DFFA-like effector B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CIDEB, check out the CIDEB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CIDEB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CIDE-B Antibody (A07404-1)
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