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Product Info Summary
| SKU: | A00102-1 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-IL-6 Antibody
SKU/Catalog Number
A00102-1
Size
100ug
Form
Lyophilized
Description
Boster Bio Anti-IL-6 Antibody (Catalog # A00102-1). Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-IL-6 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00102-1)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with mature length recombinant human IL-6 produced in E.coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00102-1 is reactive to IL6 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Observed Molecular Weight
42 kDa
Calculated molecular weight
24384 MW
Background of IL-6
Anti-IL-6 antibody is validated by ELISA, Immunohistochemistry and Western Blot Assays. IL-6 is a secreted cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response and plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. IL-6 induces myeloma and plasmacytoma growth and induces nerve cells differentiation and acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. IL-6 also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00102-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:10,000
IHC: 1:200 - 1:1,000
WB: 1:300 - 1:1,000
Validation Images & Assay Conditions
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IL6 was detected in paraffin-embedded sections of human lymph nodes tissues using rabbit anti-IL6 Antigen Affinity purified polyclonal antibody (Catalog # A00102-1) at 10 μg/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1022).
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Western blot analysis of IL-6 expression in recombinant human IL6 (lane 1). IL-6 at 24KD was detected using rabbit anti-IL-6 Antigen Affinity purified polyclonal antibody (Catalog # A00102-1) at 1:500. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
Protein Target Info & Infographic
Gene/Protein Information For IL6 (Source: Uniprot.org, NCBI)
Gene Name
IL6
Full Name
Interleukin-6
Weight
24384 MW
Superfamily
IL-6 superfamily
Alternative Names
IL-6, interleukin-6, HSF, Hybridoma growth factor, Hybridoma plasmacytoma growth factor, IFNB2, IL 6, IL6 protein, Interferon beta 2, Interleukin 6, BSF2, CDF IL6 BSF-2, BSF2, CDF, HGF, HSF, IFN-beta-2, IFNB2, IL-6 interleukin 6 interleukin-6|B-cell differentiation factor|B-cell stimulatory factor 2|CTL differentiation factor|hybridoma growth factor|interferon beta-2|interleukin BSF-2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IL6, check out the IL6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IL6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IL-6 Antibody (A00102-1)
Hello CJ!
A00102-1 has been cited in 13 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Upregulation of Toll‑like receptor 2 expression in colorectal cancer infected by human cytomegalovirus
Nifedipine inhibits oxidative stress and ameliorates osteoarthritis by activating the nuclear factor erythroid-2-related factor 2 pathway
Feng X,Yang M,Yang Z,Qian Q,Burns EM,Min W.Abnormal expression of the co-stimulatory molecule B7-H3 in lichen simplex chronicus is associated with expansion of Langerhans cells. Clin Exp Dermatol. 2020 Jan;45(1):30-35.doi:10.1111/ced.14001.Epub 2019 Jul 3
Species: Human
A00102-1 usage in article: APP:IHC, SAMPLE:SKIN TISSUE, DILUTION:NA
Distribution change of mast cells in human nasal polyps
Serum interleukin-6 is not linked with sleep-quality, restless legs syndrome, and depression, but with 6-month survival in hematological malignancies
Expression of the interleukin 6 system in cortical lesions from patients with tuberous sclerosis complex and focal cortical dysplasia type IIb
Resveratrol protects against early polymicrobial sepsis-induced acute kidney injury through inhibiting endoplasmic reticulum stress-activated NF-?B pathway
Pannexin3 inhibits TNF???induced inflammatory response by suppressing NF??B signalling pathway in human dental pulp cells
Potential implications of Apolipoprotein E in early brain injury after experimental subarachnoid hemorrhage: Involvement in the modulation of blood-brain barrier integrity
MiR674 inhibits the neuraminidase-stimulated immune response on dendritic cells via down-regulated Mbnl3
Recommended Resources
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-IL-6 Antibody
Question
We are currently using anti-IL-6 antibody A00102-1 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2018-08-10
Answer
The anti-IL-6 antibody (A00102-1) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-08-10
Question
We have observed staining in human left coronary artery. Any tips? Is anti-IL-6 antibody supposed to stain left coronary artery positively?
R. Anderson
Verified customer
Asked: 2017-09-20
Answer
According to literature left coronary artery does express IL6. According to Uniprot.org, IL6 is expressed in left coronary artery, fibroblast, lung, among other tissues. Regarding which tissues have IL6 expression, here are a few articles citing expression in various tissues:
Fibroblast, Pubmed ID: 3758081
Lung, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2017-09-20
Question
Our lab were happy with the WB result of your anti-IL-6 antibody. However we have observed positive staining in lung secreted. using this antibody. Is that expected? Could you tell me where is IL6 supposed to be expressed?
B. Li
Verified customer
Asked: 2016-08-16
Answer
Based on literature, lung does express IL6. Generally IL6 expresses in secreted. Regarding which tissues have IL6 expression, here are a few articles citing expression in various tissues:
Fibroblast, Pubmed ID: 3758081
Lung, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2016-08-16
Question
We ordered your anti-IL-6 antibody for Neu on fibroblast a few months ago. I am using human, and We intend to use the antibody for WB next. We need examining fibroblast as well as lung in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
D. Dhar
Verified customer
Asked: 2013-05-24
Answer
I took a look at the website and datasheets of our anti-IL-6 antibody and it seems that A00102-1 has been tested on human in both Neu and WB. Thus A00102-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2013-05-24
