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Product Info Summary
| SKU: | A04511 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF |
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Product info
Product Name
Anti-CHST6 Antibody
View all Carbohydrate Sulfotransferase 6/CHST6 Antibodies
SKU/Catalog Number
A04511
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-CHST6 Antibody (Catalog # A04511). Tested in IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CHST6 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04511)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human CHST6.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04511 is reactive to CHST6 in Human
Reconstitution
Calculated molecular weight
44099 MW
Background of Carbohydrate Sulfotransferase 6/CHST6
Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues of keratan. Mediates sulfation of keratan in cornea. Keratan sulfate plays a central role in maintaining corneal transparency. Acts on the non-reducing terminal GlcNAc of short and long carbohydrate substrates that have poly-N-acetyllactosamine structures.
Akama T.O., Nat. Genet. 26:237-241(2000).
Hemmerich S., Glycobiology 11:75-87(2001).
Bartes A., Biochem. Biophys. Res. Commun. 282:928-933(2001).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04511 is guaranteed for IF Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunofluorescence: 1:100~1:500
Validation Images & Assay Conditions
Click image to see more details
Immunofluorescence analysis of A549 cells, using CHST6 antibody A04511.
Protein Target Info & Infographic
Gene/Protein Information For CHST6 (Source: Uniprot.org, NCBI)
Gene Name
CHST6
Full Name
Carbohydrate sulfotransferase 6
Weight
44099 MW
Superfamily
sulfotransferase 1 family
Alternative Names
Carbohydrate sulfotransferase 6;2.8.2.-;Corneal N-acetylglucosamine-6-O-sulfotransferase;C-GlcNAc6ST;hCGn6ST;Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 4-beta;GST4-beta;N-acetylglucosamine 6-O-sulfotransferase 5;GlcNAc6ST-5;Gn6st-5;CHST6; CHST6 C-GlcNAc6ST, GST4-beta, MCDC1, glcNAc6ST-5, gn6st-5, hCGn6ST carbohydrate sulfotransferase 6 carbohydrate sulfotransferase 6|N-acetylglucosamine 6-O-sulfotransferase 5|N-acetylglucosamine 6-sulfotransferase|carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 6|corneal N-acetylglucosamine 6-sulfotransferase|corneal N-acetylglucosamine-6-O-sulfotransferase|galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 4-beta
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CHST6, check out the CHST6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CHST6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CHST6 Antibody (A04511)
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No publications found for A04511
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Customer Reviews
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Customer Q&As
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13 Customer Q&As for Anti-CHST6 Antibody
Question
Is this A04511 anti-CHST6 antibody reactive to the isotypes of CHST6?
Verified Customer
Verified customer
Asked: 2020-02-25
Answer
The immunogen of A04511 anti-CHST6 antibody is Synthesized peptide derived from internal of human CHST6. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-02-25
Question
Can you help my question with product A04511, anti-CHST6 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-21
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04511 anti-CHST6 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-21
Question
Our lab want to know about to test anti-CHST6 antibody A04511 on human bronchial epithelial cell for research purposes, then I may be interested in using anti-CHST6 antibody A04511 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-11-05
Answer
The products we sell, including anti-CHST6 antibody A04511, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-11-05
Question
Is a blocking peptide available for product anti-CHST6 antibody (A04511)?
Verified Customer
Verified customer
Asked: 2019-09-27
Answer
We do provide the blocking peptide for product anti-CHST6 antibody (A04511). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-09-27
Question
I have attached the WB image, lot number and protocol we used for bronchial epithelial cell using anti-CHST6 antibody A04511. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-07-31
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-31
Question
I see that the anti-CHST6 antibody A04511 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-16
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-07-16
Question
Is there a BSA free version of anti-CHST6 antibody A04511 available?
Verified Customer
Verified customer
Asked: 2019-05-30
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-CHST6 antibody A04511 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-05-30
Question
We are currently using anti-CHST6 antibody A04511 for human tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on goat tissues as well?
H. Brown
Verified customer
Asked: 2019-04-02
Answer
The anti-CHST6 antibody (A04511) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-04-02
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for bronchial epithelial cell using anti-CHST6 antibody A04511. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-10-23
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-10-23
Question
I was wanting to use your anti-CHST6 antibody for IF for human bronchial epithelial cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human bronchial epithelial cell identification?
Verified Customer
Verified customer
Asked: 2018-04-25
Answer
It shows on the product datasheet, A04511 anti-CHST6 antibody has been tested for IF on human tissues. We have an innovator award program that if you test this antibody and show it works in human bronchial epithelial cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-04-25
Question
Does anti-CHST6 antibody A04511 work for IF with bronchial epithelial cell?
Verified Customer
Verified customer
Asked: 2017-07-21
Answer
According to the expression profile of bronchial epithelial cell, CHST6 is highly expressed in bronchial epithelial cell. So, it is likely that anti-CHST6 antibody A04511 will work for IF with bronchial epithelial cell.
Boster Scientific Support
Answered: 2017-07-21
Question
Would anti-CHST6 antibody A04511 work on feline IF with bronchial epithelial cell?
P. Evans
Verified customer
Asked: 2016-12-09
Answer
Our lab technicians have not tested anti-CHST6 antibody A04511 on feline. You can run a BLAST between feline and the immunogen sequence of anti-CHST6 antibody A04511 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline bronchial epithelial cell in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-12-09
Question
Would A04511 anti-CHST6 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Z. Kulkarni
Verified customer
Asked: 2013-10-16
Answer
It shows on the product datasheet, A04511 anti-CHST6 antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-10-16
