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Product Info Summary
| SKU: | A00277 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-Chk2 CHEK2 Antibody
SKU/Catalog Number
A00277
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Chk2 CHEK2 Antibody (Catalog # A00277). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Chk2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Chk2 CHEK2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00277)
Host
Rabbit
Contents
Chk2 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Chk2 antibody was raised against a synthetic peptide corresponding to amino acids near the amino terminus of human Chk2. The immunogen is located within the first 50 amino acids of Chk2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross response to IKKa, IKKb, IKKy, or IKKe.
Reactive Species
A00277 is reactive to CHEK2 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
60915 MW
Background of Chk2
The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 signaling, including Chk2, p53R2, p53AIP1, Noxa, PIDD, and PID/MTA2, were recently discovered. The checkpoint kinase Chk2 is the mammalian homologue of yeast Cds1/Rad53. In response to DNA damage, the checkpoint kinase ATM phosphorylates and activates Chk2, which in turn directly phosphorylates and activates p53. Chk2 serves as ATM downstream effector to mediate activation of p53. Chk2 also phosphorylates and activates BRCA1, the product of a tumor suppressor gene that is mutated in breast and ovarian cancer.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00277 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Chk2 antibody can be used for detection of Chk2 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of Chk2 expression in (A) K562
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Immunocytochemistry of Chk2 in Jurkat cells with Chk2 antibody at 1 μg/mL.
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Immunofluorescence of Chk2 in Jurkat cells with Chk2 antibody at 5 μg/mL
Red: Chk2 Antibody (A00277)
Blue: DAPI staining
Protein Target Info & Infographic
Gene/Protein Information For CHEK2 (Source: Uniprot.org, NCBI)
Gene Name
CHEK2
Full Name
Serine/threonine-protein kinase Chk2
Weight
60915 MW
Superfamily
protein kinase superfamily
Alternative Names
CDS1, CHK2, LFS2, RAD53, hCds1, HuCds1, PP1425, CDS1, CHK2 checkpoint homolog, Hucds1 CHEK2 CDS1, CHK2, HuCds1, LFS2, PP1425, RAD53, hCds1 checkpoint kinase 2 serine/threonine-protein kinase Chk2|CHK2 checkpoint homolog|cds1 homolog|checkpoint-like protein CHK2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CHEK2, check out the CHEK2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CHEK2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Chk2 CHEK2 Antibody (A00277)
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No publications found for A00277
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