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Product Info Summary
| SKU: | M06787-2 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Chicken |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Chicken Polyoclonal Peripherin PRPH Antibody
View all Peripherin Antibodies
SKU/Catalog Number
M06787-2
Size
100ul
Description
Boster Bio Anti-Chicken Polyoclonal Peripherin PRPH Antibody catalog # M06787-2. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Chicken Polyoclonal Peripherin PRPH Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06787-2)
Host
Chicken
Contents
Supplied as an aliquot of IgY preparation plus 5mM NaN3
Clonality
Polyclonal
Immunogen
Recombinant rat peripherin expressed in and purified from E. coli
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M06787-2 is reactive to PRPH in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
53651 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M06787-2 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:20,000. IF/ICC and IHC: 1:2,000.
Validation Images & Assay Conditions
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Western blot analysis of spinal cord tissue lysates (lanes 2-5) and cell lysates (lanes 6 and 7) using chicken pAb to peripherin, M06787-2, dilution 1:10,000 in green: [1] protein standard (red), [2] rat, [3] mouse, [4] pig, [5] cow spinal cord; [6] SH-SY5Y, and [7] PC12 cells. The band at 57kDa corresponds to peripherin protein.
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Immunofluorescent analysis of mixed fibroblast and PC12 pheochromocytoma cell culture stained with chicken pAb to peripherin, M06787-2, dilution 1:5,000 in green, and costained with rabbit pAb to vimentin, dilution 1:2,000, in red. The blue is DAPI staining of nuclear DNA. M06787-2 antibody reveals cytoplasmic filamentous staining in the small PC12 cells, while vimentin antibody stains intermediate filaments in the surrounding fibroblastic cells which do not express peripherin.
Protein Target Info & Infographic
Gene/Protein Information For PRPH (Source: Uniprot.org, NCBI)
Gene Name
PRPH
Full Name
Peripherin
Weight
53651 MW
Superfamily
intermediate filament family
Alternative Names
Peripherin;Neurofilament 4;PRPH;NEF4, PRPH1; PRPH NEF41, PRPH peripherin peripherin|neurofilament 4 (57kD)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PRPH, check out the PRPH Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PRPH: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Chicken Polyoclonal Peripherin PRPH Antibody (M06787-2)
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No publications found for M06787-2
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Customer Q&As
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1 Customer Q&As for Anti-Chicken Polyoclonal Peripherin PRPH Antibody
Question
We are currently using anti-Chicken Polyoclonal Peripherin PRPH antibody M06787-2 for mouse tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on goat tissues as well?
M. Bhatt
Verified customer
Asked: 2019-10-23
Answer
The anti-Chicken Polyoclonal Peripherin PRPH antibody (M06787-2) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-23
