Product Info Summary
SKU: | PA1938 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Acid-sensing ion channel 3 ASIC3 Antibody
View all Amiloride-sensitive cation channel 3 Antibodies
SKU/Catalog Number
PA1938
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Acid-sensing ion channel 3 ASIC3 Antibody catalog # PA1938. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Acid-sensing ion channel 3 ASIC3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1938)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human ASIC3, different from the related rat and mouse sequences by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1938 is reactive to ASIC3 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
69 kDa
Calculated molecular weight
58905 MW
Background of Amiloride-sensitive cation channel 3
ASIC3 (Acid-Sensing Ion Channel3), also known as TESTIS SODIUM CHANNEL 1 (TNAC1) or DORSAL ROOT ACID-SENSING ION CHANNEL (DRASIC), is a protein that in humans is encoded by the ASIC3 gene. ASIC3 belongs to a family of acid-sensing channel proteins that are structurally related to epithelial sodium channel proteins and support acid-activated membrane currents. By radiation hybrid analysis, de Weille et al. (1998) mapped the ACCN3 gene to chromosome 7q35. De Weille et al. (1998) found that human ASIC3 supported an H (+)-gated cation current in COS cells with kinetics similar to those of rat Asic3. Babinski et al. (1999) expressed homomeric human ASIC3 channels in Xenopus oocytes and found that rapid reduction in extracellular pH resulted in a biphasic response characterized by a fast and rapidly desensitizing current followed by a slow and sustained current that returned to baseline only on return to physiologic pH.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1938 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Positive Control
WB: Rat Brain Tissue, Rat Testis Tissue, U87 Cell, NEURO Cell, SMMC Cell
FCM: U937 cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of ASIC3 using anti-ASIC3 antibody (PA1938).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Rat Brain Tissue Lysate,
Lane 2: Rat Testis Tissue Lysate,
Lane 3: U87 Cell Lysate,
Lane 4: NEURO Cell Lysate,
Lane 5: SMMC Cell Lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ASIC3 antigen affinity purified polyclonal antibody (Catalog # PA1938) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ASIC3 at approximately 69KD. The expected band size for ASIC3 is at 58KD.
Click image to see more details
Figure 2. Flow Cytometry analysis of U937 cells using anti-ASIC3 antibody (PA1938).
Overlay histogram showing U937 cells stained with PA1938 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ASIC3 Antibody (PA1938,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For ASIC3 (Source: Uniprot.org, NCBI)
Gene Name
ASIC3
Full Name
Acid-sensing ion channel 3
Weight
58905 MW
Superfamily
amiloride-sensitive sodium channel (TC 1.A.6) family
Alternative Names
Acid-sensing ion channel 3;ASIC3;hASIC3;Amiloride-sensitive cation channel 3;Neuronal amiloride-sensitive cation channel 3;Testis sodium channel 1;hTNaC1;ASIC3;ACCN3, SLNAC1, TNAC1; ASIC3 ACCN3, DRASIC, SLNAC1, TNaC1 acid sensing ion channel subunit 3 acid-sensing ion channel 3|acid sensing (proton gated) ion channel 3|amiloride-sensitive cation channel 3, testis|modulatory subunit of ASIC2a|neuronal amiloride-sensitive cation channel 3|proton-gated cation channel subunit|testis sodium channel 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ASIC3, check out the ASIC3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ASIC3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Acid-sensing ion channel 3 ASIC3 Antibody (PA1938)
Hello CJ!
PA1938 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Effect of knockout of the ASIC3 on cardiovascular reflexes arising from hindlimb muscle in decerebrated rats
Schuhmacher LN, Callejo G, Srivats S, Smith ESJ. J Biol Chem. 2018 Feb 2;293(5):1756-1766. doi: 10.1074/jbc.M117.807859. Epub 2017 Dec 13. Naked mole-rat acid-sensing ion channel 3 forms nonfunctional homomers, but functional heteromers
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Acid-sensing ion channel 3 ASIC3 Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-Acid-sensing ion channel 3 ASIC3 Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
6 Customer Q&As for Anti-Acid-sensing ion channel 3 ASIC3 Antibody
Question
We are currently using anti-ASIC3 antibody PA1938 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-14
Answer
The anti-ASIC3 antibody (PA1938) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-14
Question
Is this PA1938 anti-ASIC3 antibody reactive to the isotypes of ASIC3?
Verified Customer
Verified customer
Asked: 2019-05-17
Answer
The immunogen of PA1938 anti-ASIC3 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human ASIC3(56-73aa FLYQVAERVRYYREFHHQ), different from the related rat and mouse sequences by two amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-05-17
Question
We need to test anti-ASIC3 antibody PA1938 on rat right hemisphere of cerebellum for research purposes, then I may be interested in using anti-ASIC3 antibody PA1938 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
T. Krishna
Verified customer
Asked: 2019-04-05
Answer
The products we sell, including anti-ASIC3 antibody PA1938, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-04-05
Question
Do you have data regarding IF and IHC-P application using PA1938?
Verified customer
Asked: 2019-03-15
Answer
We did not get positive staining for the IHC-P test. We do not recommend using the Anti-Acid-Sensing Ion Channel 3 ASIC3 Antibody (PA1938) for IF and IHC-P.
Boster Scientific Support
Answered: 2019-03-18
Question
Would anti-ASIC3 antibody PA1938 work on horse WB with testis?
Verified Customer
Verified customer
Asked: 2018-03-16
Answer
Our lab technicians have not tested anti-ASIC3 antibody PA1938 on horse. You can run a BLAST between horse and the immunogen sequence of anti-ASIC3 antibody PA1938 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse testis in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-03-16
Question
I am interested in your #PA1938 ASIC3 antibody. May I know if you have data regarding to IF and IHC-P application? Please let me know if you have related information.
Verified Customer
Verified customer
Asked: 2013-12-30
Answer
We didn't get positive staining for IHC-P test. This antibody is not recommended to work for
Boster Scientific Support
Answered: 2013-12-30