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Product Info Summary
| SKU: | A01201 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-MAP2 Antibody Picoband®
SKU/Catalog Number
A01201
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MAP2 Antibody Picoband® catalog # A01201. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MAP2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01201)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human MAP2 recombinant protein (Position: Q64-L133).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01201 is reactive to MAP2 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
280 kDa
Calculated molecular weight
199.526kDa
Background of MAP2
Microtubule-associated protein 2 is a protein that in humans is encoded by the MAP2 gene. This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isoforms have been described.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01201 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: rat brain tissue, mouse brain tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of MAP2 using anti-MAP2 antibody (A01201).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP2 antigen affinity purified polyclonal antibody (Catalog # A01201) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP2 at approximately 280KD. The expected band size for MAP2 is at 199KD.
Protein Target Info & Infographic
Gene/Protein Information For MAP2 (Source: Uniprot.org, NCBI)
Gene Name
MAP2
Full Name
Microtubule-associated protein 2
Weight
199.526kDa
Alternative Names
Microtubule-associated protein 2; MAP-2; MAP2 MAP2 MAP-2A, MAP2B, MAP2C, MAP2 microtubule associated protein 2 microtubule-associated protein 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MAP2, check out the MAP2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MAP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MAP2 Antibody Picoband® (A01201)
Hello CJ!
A01201 has been cited in 20 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
The effect of acetylcholine-like biomimetic polymers on neuronal growth
Species: Rat
Wu K,Yue J,Shen K,He J,Zhu G,Liu S,Yang H,Zhang C.Expression and cellular distribution of FGF13 in cortical tubers of the tuberous sclerosis complex.Neurosci Lett. 2021 Feb 11:135714.doi:10.1016/j.neulet.2021.135714.Epub ahead of print.PMID:33582188.
Species: Human
A01201 usage in article: APP:IF, SAMPLE:BRAIN TISSUE, DILUTION:1:50
Neural stem cells over-expressing brain-derived neurotrophic factor promote neuronal survival and cytoskeletal protein expression in traumatic brain injury %u2026
Nitrobenzylthioinosine mimics adenosine to attenuate the epileptiform discharge of hippocampal neurons from epileptic rats
Association of RASgrf1 methylation with epileptic seizures
Direct reprogramming of fibroblasts into neural stem cells by single non-neural progenitor transcription factor Ptf1a
Neuroprotective effects of leonurine against oxygen?glucose deprivation by targeting Cx36/CaMKII in PC12 cells
Lithium promotes DNA stability and survival of ischemic retinal neurocytes by upregulating DNA ligase IV
Establishment of Lipofection Protocol for Efficient miR-21 Transfection into Cortical Neurons In Vitro
Activation of the macroautophagic system in scrapie-infected experimental animals and human genetic prion diseases
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-MAP2 Antibody Picoband®
Question
We are currently using anti-MAP2 antibody A01201 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-07
Answer
The anti-MAP2 antibody (A01201) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-07
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-MAP2 antibody A01201. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-01-13
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-13
Question
Is there a BSA free version of anti-MAP2 antibody A01201 available?
Verified Customer
Verified customer
Asked: 2019-11-13
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-MAP2 antibody A01201 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-11-13
Question
My team were satisfied with the WB result of your anti-MAP2 antibody. However we have seen positive staining in cervix carcinoma cytoskeleton. cell using this antibody. Is that expected? Could you tell me where is MAP2 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-11-04
Answer
Based on literature, cervix carcinoma does express MAP2. Generally MAP2 expresses in cytoplasm, cytoskeleton. cell. Regarding which tissues have MAP2 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 8294038
Brain, and Pancreas, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 17081983, 18220336
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2019-11-04
Question
We have been able to see staining in human liver. Do you have any suggestions? Is anti-MAP2 antibody supposed to stain liver positively?
K. Krishna
Verified customer
Asked: 2019-10-10
Answer
Based on literature liver does express MAP2. Based on Uniprot.org, MAP2 is expressed in dorsolateral prefrontal cortex, brain, brain pancreas, cervix carcinoma, liver, among other tissues. Regarding which tissues have MAP2 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 8294038
Brain, and Pancreas, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 17081983, 18220336
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2019-10-10
Question
Is this A01201 anti-MAP2 antibody reactive to the isotypes of MAP2?
T. Mitchell
Verified customer
Asked: 2019-08-06
Answer
The immunogen of A01201 anti-MAP2 antibody is E. coli-derived human MAP2 recombinant protein (Position: Q64-L133). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-08-06
Question
See below the WB image, lot number and protocol we used for brain using anti-MAP2 antibody A01201. Please let me know if you require anything else.
V. Collins
Verified customer
Asked: 2019-06-27
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-27
Question
Is a blocking peptide available for product anti-MAP2 antibody (A01201)?
Verified Customer
Verified customer
Asked: 2018-11-26
Answer
We do provide the blocking peptide for product anti-MAP2 antibody (A01201). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-11-26
Question
We purchased anti-MAP2 antibody for WB on dorsolateral prefrontal cortex in a previous project. I am using mouse, and We intend to use the antibody for ELISA next. My lab would like examining dorsolateral prefrontal cortex as well as liver in our next experiment. Do you have any suggestion on which antibody would work the best for ELISA?
A. Zhao
Verified customer
Asked: 2018-08-13
Answer
I looked at the website and datasheets of our anti-MAP2 antibody and I see that A01201 has been validated on mouse in both WB and ELISA. Thus A01201 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-08-13
Question
Does anti-MAP2 antibody A01201 work for ELISA with brain?
E. Wu
Verified customer
Asked: 2018-05-22
Answer
According to the expression profile of brain, MAP2 is highly expressed in brain. So, it is likely that anti-MAP2 antibody A01201 will work for ELISA with brain.
Boster Scientific Support
Answered: 2018-05-22
Question
Will A01201 anti-MAP2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-05-07
Answer
You can see on the product datasheet, A01201 anti-MAP2 antibody as been validated on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-05-07
Question
I see that the anti-MAP2 antibody A01201 works with ELISA, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-09-14
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-09-14
Question
you antibody using your anti-MAP2 antibody for regulation of cellular protein localization studies. Has this antibody been tested with western blotting on rat brain tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2017-05-15
Answer
Thanks for your inquiry. This A01201 anti-MAP2 antibody is validated on rat brain tissue, mouse brain. It is guaranteed to work for ELISA, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-05-15
Question
Can you help my question with product A01201, anti-MAP2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
M. Jones
Verified customer
Asked: 2016-02-11
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01201 anti-MAP2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-02-11
Question
I was wanting to use your anti-MAP2 antibody for ELISA for mouse brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse brain identification?
W. Krishna
Verified customer
Asked: 2014-06-13
Answer
As indicated on the product datasheet, A01201 anti-MAP2 antibody has been tested for ELISA, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-06-13
Question
I am interested in to test anti-MAP2 antibody A01201 on mouse brain for research purposes, then I may be interested in using anti-MAP2 antibody A01201 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
M. Zhao
Verified customer
Asked: 2013-05-13
Answer
The products we sell, including anti-MAP2 antibody A01201, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-05-13
