Anti-cGKI/PRKG1 Antibody Picoband®

cGK1/PRKG1 antibody

Boster Bio Anti-cGKI/PRKG1 Antibody Picoband® catalog # A01708-3. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: A01708-3
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, Flow Cytometry, IF, IHC, ICC, WB

Product Name

Anti-cGKI/PRKG1 Antibody Picoband®

View all cGK1/PRKG1 Antibodies

SKU/Catalog Number

A01708-3

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-cGKI/PRKG1 Antibody Picoband® catalog # A01708-3. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-cGKI/PRKG1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01708-3)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human cGKI/PRKG1 recombinant protein (Position: S2-Q44).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01708-3 is reactive to PRKG1 in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

78 kDa

Calculated molecular weight

76.364kDa

Background of cGK1/PRKG1

cGMP-dependent protein kinase 1, alpha isozyme is an enzyme that in humans is encoded by the PRKG1 gene. Mammals have three different isoforms of cyclic GMP-dependent protein kinase (Ialpha, Ibeta, and II). These PRKG isoforms act as key mediators of the nitric oxide/cGMP signaling pathway and are important components of many signal transduction processes in diverse cell types. This PRKG1 gene on human chromosome 10 encodes the soluble Ialpha and Ibeta isoforms of PRKG by alternative transcript splicing. A separate gene on human chromosome 4, PRKG2, encodes the membrane-bound PRKG isoform II. The PRKG1 proteins play a central role in regulating cardiovascular and neuronal functions in addition to relaxing smooth muscle tone, preventing platelet aggregation, and modulating cell growth. This gene is most strongly expressed in all types of smooth muscle, platelets, cerebellar Purkinje cells, hippocampal neurons, and the lateral amygdala. Isoforms Ialpha and Ibeta have identical cGMP-binding and catalytic domains but differ in their leucine/isoleucine zipper and autoinhibitory sequences and therefore differ in their dimerization substrates and kinase enzyme activity.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A01708-3 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human, Rat
ELISA, 0.1-0.5μg/ml, -

Positive Control

WB: rat lung tissue, rat heart tissue, mouse lung tissue, human HEK293 whole cell
IHC: mouse lung tissue, rat lung tissue, hman breast cancer tissue
ICC/IF: U20S cell
FCM: RH35 cell, U251 cell

Validation Images & Assay Conditions

Gene/Protein Information For PRKG1 (Source: Uniprot.org, NCBI)

Gene Name

PRKG1

Full Name

cGMP-dependent protein kinase 1

Weight

76.364kDa

Superfamily

protein kinase superfamily

Alternative Names

E3 SUMO-protein ligase PIAS1; DEAD/H box-binding protein 1; E3 SUMO-protein transferase PIAS1; Gu-binding protein; GBP; Protein inhibitor of activated STAT protein 1; RNA helicase II-binding protein; PIAS1; DDXBP1 PRKG1 AAT8, PKG, PKG1B, PRKGR1B, cGK, cGK 1, cGK1, cGKI, cGKI-BETA, cGKI-alpha, PRKG1 protein kinase cGMP-dependent 1 cGMP-dependent protein kinase 1|protein kinase, cGMP-dependent, regulatory, type I, beta|protein kinase, cGMP-dependent, type I

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PRKG1, check out the PRKG1 Infographic

PRKG1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PRKG1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Order DetailsPrice
A01708-3

100μg

$370
A01708-3-10ug

10μg sample (liquid)

$99
A01708-3-Biotin

100 μg Biotin conjugated

$570
A01708-3-Cy3

100 μg Cy3 conjugated

$570
A01708-3-Dylight488

100 μg Dylight488 conjugated

$570
A01708-3-Dylight550

100 μg Dylight550 conjugated

$570
A01708-3-Dylight594

100 μg Dylight594 conjugated

$570
A01708-3-FITC

100 μg FITC conjugated

$570
A01708-3-HRP

100 μg HRP conjugated

$570
A01708-3-APC

100 μg APC conjugated

$670
A01708-3-PE

100 μg PE conjugated

$670
A01708-3-iFluor647

100 μg iFluor647 conjugated

$670
A01708-3-carrier-free

Carrier Free

$370

More conjugates/formats

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A01708-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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