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Product Info Summary
| SKU: | A30481 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB, IF (paraffin) |
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Product info
Product Name
Anti-CGK 2 Antibody
SKU/Catalog Number
A30481
Size
100ul
Form
Liquid
Description
Boster Bio Anti-CGK 2 Antibody (Catalog# A30481). Tested in WB, IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CGK 2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30481)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human CGK 2. Immunogen sequence location: 391-440
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A30481 is reactive to PRKG2 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
72 kDa
Calculated molecular weight
87432 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30481 is guaranteed for ELISA, IHC-P, WB, IF (paraffin) Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
ELISA, 1:20000
Not yet tested in other applications.
Validation Images & Assay Conditions
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Western blot analysis of lysates from HUVEC cells, using CGK 2 Antibody. The lane on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For PRKG2 (Source: Uniprot.org, NCBI)
Gene Name
PRKG2
Full Name
cGMP-dependent protein kinase 2
Weight
87432 MW
Superfamily
protein kinase superfamily
Alternative Names
PRKG2;PRKGR2;cGMP-dependent protein kinase 2;cGK 2;cGK2;cGMP-dependent protein kinase II;cGKII PRKG2 PKG2, PRKGR2, cGK2, cGKII protein kinase cGMP-dependent 2 cGMP-dependent protein kinase 2|cGMP-dependent protein kinase II|protein kinase, cGMP-dependent, type II|testicular tissue protein Li 99
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PRKG2, check out the PRKG2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PRKG2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CGK 2 Antibody (A30481)
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No publications found for A30481
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-CGK 2 Antibody
Question
Do you have a BSA free version of anti-CGK 2 antibody A30481 available?
Verified Customer
Verified customer
Asked: 2019-10-07
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CGK 2 antibody A30481 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-10-07
Question
I was wanting to use your anti-CGK 2 antibody for WB for rat intestine on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat intestine identification?
Verified Customer
Verified customer
Asked: 2018-12-21
Answer
As indicated on the product datasheet, A30481 anti-CGK 2 antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat intestine in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-12-21
Question
Would A30481 anti-CGK 2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
A. Anderson
Verified customer
Asked: 2018-11-06
Answer
You can see on the product datasheet, A30481 anti-CGK 2 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-11-06
Question
We are currently using anti-CGK 2 antibody A30481 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2018-09-26
Answer
The anti-CGK 2 antibody (A30481) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-09-26
