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Product Info Summary
| SKU: | A05599 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Cenexin-1 ODF2 Antibody
SKU/Catalog Number
A05599
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-Cenexin-1 ODF2 Antibody (Catalog # A05599). Tested in ELISA, WB applications. This antibody reacts with Mouse.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-Cenexin-1 ODF2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05599)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a truncated recombinant protein hCenexin1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A05599 is reactive to ODF2 in Mouse
Reconstitution
Observed Molecular Weight
42 kDa
Calculated molecular weight
95.401kDa
Background of ODF2
This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. Cenexin-1 (ODF84) and cenexin-2 are splicing variants that are expressed from the same genetic locus Outer Dense Fiber protein 2 (ODF2). Cenexin-1 and cenexin-2, regarded as centriolar proteins. Cenexin-1, a 93-kD variant of ODF2, is the major form of cennexin expressed in various somatic cells and tissues and associates with centrosomes. Cenexin-1 contains a unique C-terminal extension that appears to play a critical role in recruiting mitotic regulators such as polo-like kinase 1. In contrast, ODF2, a 70-kDa protein, is a major component of sperm tail cytoskeleton and abundantly expressed in testis. Additional evidence suggests that both of these proteins play important role in primary cilia formation. The fibers function in maintaining the elastic structure and recoil of the sperm tail as well as in protecting the tail from shear forces during epididymal transport and ejaculation. Defects in the outer dense fibers lead to abnormal sperm morphology and infertility. Cenexin-1 is one of the major outer dense fiber proteins. Multiple protein isoforms are encoded by transcript variants of the cenexin gene; however, not all isoforms and variants have been fully described.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05599 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:500 - 1:10,000
WB: 1:500-1:2000
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of ODF2 using anti-ODF2 antibody (A05599).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ODF2 antigen affinity purified polyclonal antibody (Catalog # A05599) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for ODF2.
Protein Target Info & Infographic
Gene/Protein Information For ODF2 (Source: Uniprot.org, NCBI)
Gene Name
ODF2
Full Name
Outer dense fiber protein 2
Weight
95.401kDa
Superfamily
ODF2 family
Alternative Names
Cenexin-1, ODF84, Outer dense fiber of sperm tail 2, Cenexin1 variant 1, KKT 4, Cenexin 1 ODF2 CT134/1, ODF2/2, ODF84, ODF2 outer dense fiber of sperm tails 2 outer dense fiber protein 2|cancer/testis 134|cenexin 1|outer dense fiber of sperm tails, 84-kD|sperm tail structural protein|testis tissue sperm-binding protein Li 51e
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ODF2, check out the ODF2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ODF2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cenexin-1 ODF2 Antibody (A05599)
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Customer Q&As
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1 Customer Q&As for Anti-Cenexin-1 ODF2 Antibody
Question
Is this A05599 anti-Cenexin-1 antibody reactive to the isotypes of ODF2?
Verified Customer
Verified customer
Asked: 2018-09-03
Answer
The immunogen of A05599 anti-Cenexin-1 antibody is This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a truncated recombinant protein hCenexin1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-09-03
