Anti-BIN3 Monoclonal Antibody

Product Name

Anti-BIN3 Monoclonal Antibody

View all BIN3 Antibodies

SKU/Catalog Number

M11817

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-BIN3 Monoclonal Antibody (Catalog # M11817). Tested in ELISA, WB applications. This antibody reacts with Mouse.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-BIN3 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M11817)

Host

Mouse

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Monoclonal

Clone Number

Clone: 3A4

Isotype

IgG1

Immunogen

Anti-BIN3 (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with a full length human BIN3 recombinant protein followed by hybridoma development.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M11817 is reactive to BIN3 in Mouse

Applications

M11817 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

29665 MW

Background of BIN3

BIN-3 encodes an evolutionarily conserved and ubiquitously expressed member of the BAR superfamily of curved membrane and GTPase-binding proteins implicated in signal transduction and vesicular trafficking. In humans, Bin3 maps to chromosome 8p21.3, a region widely implicated in cancer suppression that is often deleted in non–Hodgkin’s lymphomas and various epithelial tumors. Study in mouse suggests that homozygous inactivation of Bin3 causes cataracts and an increased susceptibility to lymphomas during aging.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:5000 - 1:50000
IP: 10-100 µL
WB: 1:500-1:1500

Validation Images & Assay Conditions

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Western blot analysis of BIN3 expression in MEF lysate BIN3 +/-(lane 1) and MEF lysate BIN3 -/-(lane 2). BIN3 at 31KD was detected using mouse anti-BIN3 Antigen Affinity purified monoclonal antibody (Catalog # M11817) at 1:400. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1001).

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Western blot analysis of BIN3 expression in MEF cells wild type (lane 1) and MEF cells null mice (lane 2). BIN3 at 30KD was detected using mouse anti-BIN3 Antigen Affinity purified monoclonal antibody (Catalog # M11817). The blot was developed using chemiluminescence (ECL) method (Catalog # EK1001).

Gene/Protein Information For BIN3 (Source: Uniprot.org, NCBI)

Alternative Names

bridging integrator 3; MGC14978 BIN3 bridging integrator 3 bridging integrator 3

For more info on BIN3, check out the BIN3 Infographic

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In this infographic, you will see the following information for BIN3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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