Product Info Summary
SKU: | A03102 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IHC-P, IF (paraffin) |
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Product info
Product Name
Anti-CDK5RAP2 Antibody
SKU/Catalog Number
A03102
Size
100ul
Form
Liquid
Description
Boster Bio Anti-CDK5RAP2 Antibody (Catalog# A03102). Tested in IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Rat, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CDK5RAP2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03102)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human CDK5RAP2. Immunogen sequence location: 251-300
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A03102 is reactive to CDK5RAP2 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
72 kDa
Calculated molecular weight
215038 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03102 is guaranteed for ELISA, IHC-P, IF (paraffin) Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry, 1:100 - 1:300
ELISA, 1:20000
Not yet tested in other applications.
Validation Images & Assay Conditions
Click image to see more details
Immunohistochemistry analysis of paraffin-embedded human brain, using CDK5RAP2 Antibody. The picture on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For CDK5RAP2 (Source: Uniprot.org, NCBI)
Gene Name
CDK5RAP2
Full Name
CDK5 regulatory subunit-associated protein 2
Weight
215038 MW
Alternative Names
CDK5RAP2;CEP215;KIAA1633;CDK5 regulatory subunit-associated protein 2;CDK5 activator-binding protein C48;Centrosome-associated protein 215 CDK5RAP2 C48, Cep215, MCPH3 CDK5 regulatory subunit associated protein 2 CDK5 regulatory subunit-associated protein 2|CDK5 activator-binding protein C48|centrosomal protein 215 kDa|centrosomin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDK5RAP2, check out the CDK5RAP2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDK5RAP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CDK5RAP2 Antibody (A03102)
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No publications found for A03102
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Customer Q&As
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5 Customer Q&As for Anti-CDK5RAP2 Antibody
Question
I have a question about product A03102, anti-CDK5RAP2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-03
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A03102 anti-CDK5RAP2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-03
Question
I see that the anti-CDK5RAP2 antibody A03102 works with IHC, what is the protocol used to produce the result images on the product page?
K. Jones
Verified customer
Asked: 2019-05-08
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-05-08
Question
We are currently using anti-CDK5RAP2 antibody A03102 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on zebrafish tissues as well?
A. Collins
Verified customer
Asked: 2016-03-15
Answer
The anti-CDK5RAP2 antibody (A03102) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-03-15
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-CDK5RAP2 antibody A03102. Let me know if you need anything else.
G. Baker
Verified customer
Asked: 2015-05-29
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-05-29
Question
Is this A03102 anti-CDK5RAP2 antibody reactive to the isotypes of CDK5RAP2?
A. Baker
Verified customer
Asked: 2014-05-16
Answer
The immunogen of A03102 anti-CDK5RAP2 antibody is A peptide derived from human CDK5RAP2. Immunogen sequence location: 251-300. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-05-16