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Product Info Summary
| SKU: | A13764-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Cdc50B TMEM30B Antibody
SKU/Catalog Number
A13764-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Cdc50B TMEM30B Antibody catalog # A13764-1. Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cdc50B TMEM30B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13764-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human TMEM30B. AA range:91-140
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A13764-1 is reactive to TMEM30B in Human, Mouse
Reconstitution
Calculated molecular weight
38941 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A13764-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of K562 cells using Cdc50B Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For TMEM30B (Source: Uniprot.org, NCBI)
Gene Name
TMEM30B
Full Name
Cell cycle control protein 50B
Weight
38941 MW
Superfamily
CDC50/LEM3 family
Alternative Names
Cell cycle control protein 50B;P4-ATPase flippase complex beta subunit TMEM30B;Transmembrane protein 30B;TMEM30B;CDC50B; TMEM30B CDC50B transmembrane protein 30B cell cycle control protein 50B|P4-ATPase flippase complex beta subunit TMEM30B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TMEM30B, check out the TMEM30B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TMEM30B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cdc50B TMEM30B Antibody (A13764-1)
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No publications found for A13764-1
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Customer Q&As
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5 Customer Q&As for Anti-Cdc50B TMEM30B Antibody
Question
Will A13764-1 anti-Cdc50B antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-05-04
Answer
It shows on the product datasheet, A13764-1 anti-Cdc50B antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-05-04
Question
Is this A13764-1 anti-Cdc50B antibody reactive to the isotypes of TMEM30B?
Verified Customer
Verified customer
Asked: 2020-03-18
Answer
The immunogen of A13764-1 anti-Cdc50B antibody is Synthesized peptide derived from the Internal region of human Cdc50B. at AA rangle: 60-140. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-03-18
Question
My question regarding product A13764-1, anti-Cdc50B antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-03-10
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A13764-1 anti-Cdc50B antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-03-10
Question
We are currently using anti-Cdc50B antibody A13764-1 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-26
Answer
The anti-Cdc50B antibody (A13764-1) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-26
Question
Is a blocking peptide available for product anti-Cdc50B antibody (A13764-1)?
Verified Customer
Verified customer
Asked: 2018-06-04
Answer
We do provide the blocking peptide for product anti-Cdc50B antibody (A13764-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-06-04
