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Product Info Summary
| SKU: | A08803-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-SEZ6L Antibody Picoband®
SKU/Catalog Number
A08803-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SEZ6L Antibody Picoband® catalog # A08803-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SEZ6L Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08803-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human SEZ6L recombinant protein (Position: E662-E953).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A08803-1 is reactive to SEZ6L in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
112 kDa
Calculated molecular weight
111.782kDa
Background of SEZ6L
Seizure 6-like protein is a protein that in humans is encoded by the SEZ6L gene. SEZ6L (Seizure 6-Like protein; also BSRP-B in rodent) is a 200 kDa member of the SEZ6 family of proteins. It is expressed in the plasma membrane and ER of CNS neurons and lung bronchiolar epithelium, and likely regulates PKC alpha activation. Mature human SEZ6L is a 996 amino acid (aa) type I transmembrane protein. It contains a 930 aa extracellular domain (ECD) (aa 29-958) plus a 45 aa cytoplasmic tail. The ECD shows three CUB domains and five short consensus repeats (SCRs). There are multiple isoforms possible for SEZ6L. One shows a deletion of aa 933-943, and a second shows the same deletion coupled to a Thr substitution for aa 803-867. A third isoform contains a Leu substitution for aa 867-943. An alternative start site is reported at Met228. This start site may occur individually, or be accompanied by a deletion of aa 932-944, the same deletion of aa 932-944 coupled to a Thr substitution for aa 803-867, or a duel deletion of aa 869-945 and 1016-1024. Over aa 29-958, human BSRP-B is 80% aa identical to mouse BSRP-B.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08803-1 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human HEK293 whole cell, human THP-1 whole cell, human HL-60 whole cell, rat brain tissue, mouse brain tissue
ICC/IF: U20S cell
FCM: U20S cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SEZ6L using anti-SEZ6L antibody (A08803-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HEK293 whole cell lysates,
Lane 2: human THP-1 whole cell lysates,
Lane 3: human HL-60 whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: mouse brain tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEZ6L antigen affinity purified polyclonal antibody (Catalog # A08803-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SEZ6L at approximately 112KD. The expected band size for SEZ6L is at 112KD.
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Figure 2. IF analysis of SEZ6L using anti-SEZ6L antibody (A08803-1).
SEZ6L was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-SEZ6L Antibody (A08803-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 3. Flow Cytometry analysis of U20S cells using anti-SEZ6L antibody (A08803-1).
Overlay histogram showing U20S cells stained with A08803-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SEZ6L Antibody (A08803-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For SEZ6L (Source: Uniprot.org, NCBI)
Gene Name
SEZ6L
Full Name
Seizure 6-like protein
Weight
111.782kDa
Superfamily
SEZ6 family
Alternative Names
Protein phosphatase 1E; Ca (2+)/calmodulin-dependent protein kinase phosphatase N; CaMKP-N; CaMKP-nucleus; CaMKN; Partner of PIX 1; Partner of PIX-alpha; Partner of PIXA; PPM1E; CAMKN; KIAA1072; POPX1 SEZ6L seizure related 6 homolog like seizure 6-like protein|seizure related gene 6-like
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SEZ6L, check out the SEZ6L Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SEZ6L: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SEZ6L Antibody Picoband® (A08803-1)
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