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Product Info Summary
| SKU: | P03905 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Dot blot, ELISA, WB |
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Product info
Product Name
Anti-CDC27 phospho T244 Antibody
SKU/Catalog Number
P03905
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-CDC27 phospho T244 Antibody (Catalog # P03905). Tested in Dot blot, ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-CDC27 phospho T244 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P03905)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion surrounding T244 of Human CDC27.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
P03905 is reactive to CDC27, D0S1430E in Human
Reconstitution
Observed Molecular Weight
42 kDa
Calculated molecular weight
127421 MW
Background of CDC27
Human CDC27 (also called Cell division cycle protein 27 homolog, CDC27Hs and H-NUC) shares strong similarity with Saccharomyces cerevisiae protein Cdc27. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. APC catalyzes the formation of a cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P03905 is guaranteed for Dot blot, ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:8,000 - 1:30,000
IP: 1:100
WB: 1:300 - 1:2,000
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CDC27, D0S1430E using anti-CDC27, D0S1430E antibody (P03905).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDC27, D0S1430E antigen affinity purified polyclonal antibody (Catalog # P03905) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for CDC27, D0S1430E.
Protein Target Info & Infographic
Gene/Protein Information For CDC27, D0S1430E (Source: Uniprot.org, NCBI)
Gene Name
CDC27, D0S1430E
Full Name
Cell division cycle protein 27 homolog
Weight
127421 MW
Superfamily
APC3/CDC27 family
Alternative Names
CDC27 pT244, CDC 27, CDC-27, Cell division cycle protein 27 homolog, H-NUC, APC3, Anaphase-promoting complex subunit 3, ANAPC3 CDC27 ANAPC3, APC3Hs, D0S1430E, D17S978E, H-NUC, HNUC, NUC2, CDC27 cell division cycle 27 cell division cycle protein 27 homolog|D0S1430E, D17S978E|anaphase promoting complex subunit 3|anaphase-promoting complex, protein 3|cell division cycle 27 homolog|nuc2 homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDC27, D0S1430E, check out the CDC27, D0S1430E Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDC27, D0S1430E: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CDC27 phospho T244 Antibody (P03905)
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1 Customer Q&As for Anti-CDC27 phospho T244 Antibody
Question
We are currently using anti-CDC27 phospho T244 antibody P03905 for human tissue, and we are content with the IP results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on feline tissues as well?
P. Huang
Verified customer
Asked: 2013-02-13
Answer
The anti-CDC27 phospho T244 antibody (P03905) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-02-13
