Anti-CDC27 phospho T244 Antibody

Product Name

Anti-CDC27 phospho T244 Antibody

View all CDC27 Antibodies

SKU/Catalog Number

P03905

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-CDC27 phospho T244 Antibody (Catalog # P03905). Tested in Dot blot, ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-CDC27 phospho T244 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P03905)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion surrounding T244 of Human CDC27.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

P03905 is reactive to CDC27 in Human

Applications

P03905 is guaranteed for Dot blot, ELISA, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

127421 MW

Background of CDC27

Human CDC27 (also called Cell division cycle protein 27 homolog, CDC27Hs and H-NUC) shares strong similarity with Saccharomyces cerevisiae protein Cdc27. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. APC catalyzes the formation of a cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:8,000 - 1:30,000
IP: 1:100
WB: 1:300 - 1:2,000

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of CDC27, D0S1430E using anti-CDC27, D0S1430E antibody (P03905).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDC27, D0S1430E antigen affinity purified polyclonal antibody (Catalog # P03905) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for CDC27, D0S1430E.

Gene/Protein Information For CDC27 (Source: Uniprot.org, NCBI)

Alternative Names

ANAPC3CDC27 homolog; anaphase promoting complex subunit 3; Anaphase-promoting complex subunit 3; anaphase-promoting complex, protein 3; APC 3; APC3cell division cycle 27; CDC27Hs; cell division cycle 27 homolog (S. cerevisiae); cell division cycle protein 27 homolog; D0S1430E; D17S978E; HNUC; H-NUC; nuc2 homolog; NUC2 CDC27 ANAPC3, APC3Hs, D0S1430E, D17S978E, H-NUC, HNUC, NUC2, CDC27 cell division cycle 27 cell division cycle protein 27 homolog|D0S1430E, D17S978E|anaphase promoting complex subunit 3|anaphase-promoting complex, protein 3|cell division cycle 27 homolog|nuc2 homolog

For more info on CDC27, check out the CDC27 Infographic

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In this infographic, you will see the following information for CDC27: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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