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Product Info Summary
| SKU: | M05798 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-Cdc23/APC8 Rabbit Monoclonal Antibody
SKU/Catalog Number
M05798
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Cdc23/APC8 Rabbit Monoclonal Antibody catalog # M05798. Tested in WB, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cdc23/APC8 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05798)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
IAA-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Cdc23/APC8
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M05798 is reactive to CDC23 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
55 kDa
Calculated molecular weight
68834 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05798 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ICC/IF 1:50-1:100
Positive Control
WB: HepG2 cell, Jurkat cell
Validation Images & Assay Conditions
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Western blot analysis of Cdc23/APC8 expression in (1)HepG2 cell lysate; (2)Jurkat cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For CDC23 (Source: Uniprot.org, NCBI)
Gene Name
CDC23
Full Name
Cell division cycle protein 23 homolog
Weight
68834 MW
Superfamily
APC8/CDC23 family
Alternative Names
Cell division cycle protein 23 homolog;Anaphase-promoting complex subunit 8;APC8;Cyclosome subunit 8;CDC23;ANAPC8; CDC23 ANAPC8, APC8, CUT23 cell division cycle 23 cell division cycle protein 23 homolog|anaphase-promoting complex subunit 8|cell division cycle 23 homolog|cyclosome subunit 8
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDC23, check out the CDC23 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDC23: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cdc23/APC8 Rabbit Monoclonal Antibody (M05798)
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No publications found for M05798
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Customer Q&As
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4 Customer Q&As for Anti-Cdc23/APC8 Rabbit Monoclonal Antibody
Question
Does anti-Cdc23/APC8 Rabbit Monoclonal antibody M05798 work on primate WB with cervix carcinoma erythroleukemia?
Verified Customer
Verified customer
Asked: 2019-11-14
Answer
Our lab technicians have not tested anti-Cdc23/APC8 Rabbit Monoclonal antibody M05798 on primate. You can run a BLAST between primate and the immunogen sequence of anti-Cdc23/APC8 Rabbit Monoclonal antibody M05798 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate cervix carcinoma erythroleukemia in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-14
Question
Here is the WB image, lot number and protocol we used for liver using anti-Cdc23/APC8 Rabbit Monoclonal antibody M05798. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-05-31
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-31
Question
We are currently using anti-Cdc23/APC8 Rabbit Monoclonal antibody M05798 for human tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2018-08-30
Answer
The anti-Cdc23/APC8 Rabbit Monoclonal antibody (M05798) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-08-30
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-Cdc23/APC8 Rabbit Monoclonal antibody M05798. Let me know if you need anything else.
B. Johnson
Verified customer
Asked: 2017-02-06
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-02-06
