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Product Info Summary
| SKU: | A05798-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, ICC, WB |
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Product info
Product Name
Anti-APC8 CDC23 Antibody
SKU/Catalog Number
A05798-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-APC8 CDC23 Antibody (Catalog # A05798-1). Tested in ELISA, WB, ICC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
APC8 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-APC8 CDC23 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05798-1)
Host
Rabbit
Contents
APC8 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
APC8 antibody was raised against a 17 amino acid synthetic peptide near the center of human APC8. The immunogen is located within amino acids 270 - 320 of APC8.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
PHOX2A antibody is predicted to not cross-react with other paired homeobox family members.
Reactive Species
A05798-1 is reactive to CDC23 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
68834 MW
Background of CDC23
Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC8 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. In Drosophila, silencing of APC8 results in developmental delay and pupal lethality with elevated levels of apoptosis, high mitotic index, and delayed or blocked mitosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05798-1 is guaranteed for ELISA, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
APC8 antibody can be used for detection of APC8 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of APC8 in K562 cell lysate with APC8 antibody at (A) 1 and (B) 2 μg/mL.
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Immunocytochemistry of APC8 in K562 cells with APC8 antibody at 5 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For CDC23 (Source: Uniprot.org, NCBI)
Gene Name
CDC23
Full Name
Cell division cycle protein 23 homolog
Weight
68834 MW
Superfamily
APC8/CDC23 family
Alternative Names
APC8, CUT23, ANAPC8, Cell division cycle protein 23 homolog, Anaphase-promoting complex subunit 8, APC8 CDC23 ANAPC8, APC8, CUT23 cell division cycle 23 cell division cycle protein 23 homolog|anaphase-promoting complex subunit 8|cell division cycle 23 homolog|cyclosome subunit 8
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDC23, check out the CDC23 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDC23: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-APC8 CDC23 Antibody (A05798-1)
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