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Product Info Summary
| SKU: | A01724-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-CD99 Antibody Picoband®
SKU/Catalog Number
A01724-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD99 Antibody Picoband® catalog # A01724-2. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-CD99 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01724-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human CD99.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
A01724-2 is reactive to CD99 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
28 kDa
Calculated molecular weight
57954 MW
Background of CD99
CD99 antigen (Cluster of differentiation 99), also known as MIC2 or single-chain type-1 glycoprotein, is a heavily O-glycosylated transmembrane protein that is encoded by the CD99 gene in humans. The protein encoded by this gene is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. There is a related pseudogene located immediately adjacent to this locus.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01724-2 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Positive Control
WB: human MOLT-4 whole cell, human HepG2 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CD99 using anti-CD99 antibody (A01724-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MOLT-4 whole cell lysates,
Lane 2: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD99 antigen affinity purified polyclonal antibody (Catalog # A01724-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD99 at approximately 28 kDa. The expected band size for CD99 is at 28 kDa.
Protein Target Info & Infographic
Gene/Protein Information For CD99 (Source: Uniprot.org, NCBI)
Gene Name
CD99
Full Name
CD99 antigen
Weight
57954 MW
Superfamily
CD99 family
Alternative Names
Alkaline phosphatase, placental type;3.1.3.1;Alkaline phosphatase Regan isozyme;Placental alkaline phosphatase 1;PLAP-1;ALPP;PLAP; CD99 HBA71, MIC2, MIC2X, MIC2Y, MSK5X CD99 molecule (Xg blood group) CD99 |E2 |MIC2 ( 12E7)|T-cell surface glycoprotein E2| by 12E7, Y homolog| by antibodies 12E7, F21 and O13|cell surface 12E7|cell surface HBA-71|cell surface O13|surface MIC2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD99, check out the CD99 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD99: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD99 Antibody Picoband® (A01724-2)
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