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Product Info Summary
| SKU: | A00220 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-CD86 Antibody
SKU/Catalog Number
A00220
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-CD86 Antibody (Catalog # A00220). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.
Storage & Handling
CD86 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-CD86 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00220)
Host
Rabbit
Contents
CD86 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
CD86 antibody was raised against a peptide corresponding to 17 amino acids near the center of human CD86. The immunogen is located within amino acids 160 - 210 of CD86.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least five isoforms of CD86 are known to exist; this antibody will detect all five isoforms.
Reactive Species
A00220 is reactive to CD86 in Human
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
34666 MW
Background of B7-2/CD86
CD86, also known as B7-2, is a type I membrane protein that is a member of the immunoglobulin superfamily. Like the related protein CD80, this protein is expressed by antigen-presenting cells, and is the ligand for two proteins at the cell surface of T cells, CD28 and the cytotoxic T-lymphocyte-associated protein 4 (CTLA-4). Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell and induces T-cell proliferation and cytokine production. CTLA-4 binding negatively regulates T-cell activation and diminishes the immune response (1). Blocking the CTLA-4-CD80/CD86 interaction has been shown to enhance T-cell functions in acute lymphoblastomic leukemia (ALL), suggesting that this pathway may be an attractive target for future cancer immunotherapy (2).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00220 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
CD86 antibody can be used for Western blot at 1 - 2 μg/mL. Antibody can also be used for Immunohistochemistry at 2 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of CD86 in (A) Daudi and (B) Raji cell lysate with CD86 antibody at 1 μg/mL.
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Immunohistochemistry of CD86 in human lymph node tissue with CD86 antibody at 2 μg/ml.
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Immunofluorescence of CD86 in human lymph node tissue with CD86 antibody at 20 μg/ml.
Red: CD86 Antibody (A00220)
Blue: DAPI staining
Protein Target Info & Infographic
Gene/Protein Information For CD86 (Source: Uniprot.org, NCBI)
Gene Name
CD86
Full Name
T-lymphocyte activation antigen CD86
Weight
34666 MW
Alternative Names
CD86 molecule, B70, B7-2, B7.2, LAB72, CD28LG2 CD86 B7-2, B7.2, B70, CD28LG2, LAB72 CD86 molecule T-lymphocyte activation CD86|B-lymphocyte activation B7-2|BU63|CD86 (CD28 ligand 2, B7-2 )|CTLA-4 counter-receptor B7.2|FUN-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD86, check out the CD86 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD86: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD86 Antibody (A00220)
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