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Product Info Summary
| SKU: | A04872-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IHC, ICC |
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Product info
Product Name
Anti-CD84 Antibody
View all CD84/SLAMF5 Antibodies
SKU/Catalog Number
A04872-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD84 Antibody catalog # A04872-2. Tested in ELISA, Flow Cytometry, IHC, ICC applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD84 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04872-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human CD84 recombinant protein (Position: K22-G225).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A04872-2 is reactive to CD84 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
19 kDa
Calculated molecular weight
35883 MW
Background of CD84/SLAMF5
CD84 (Cluster of Differentiation 84) is a human protein encoded by the CD84 gene. This gene encodes a membrane glycoprotein that is a member of the signaling lymphocyte activation molecule (SLAM) family. This family forms a subset of the larger CD2 cell-surface receptor Ig superfamily. The encoded protein is a homophilic adhesion molecule that is expressed in numerous immune cells types and is involved in regulating receptor-mediated signaling in those cells. Alternate splicing results in multiple transcript variants. Members of the CD2 subgroup of the Ig superfamily, such as CD84, have similar patterns of conserved disulfide bonds and function in adhesion interactions between T lymphocytes and accessory cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04872-2 is guaranteed for ELISA, Flow Cytometry, IHC, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Frozen Section), 0.5-1μg/ml
Immunocytochemistry, 0.5-1μg/ml
Flow Cytometry (Fixed), 1-3μg/1x106 cells
ELISA, 0.1-0.5μg/ml
Positive Control
FCM: THP-1 cell, JK cell
IHC: human tonsil tissue
Validation Images & Assay Conditions
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Figure 1. Flow Cytometry analysis of THP-1 cells using anti-CD84 antibody (A04872-2).
Overlay histogram showing THP-1 cells stained with A04872-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD84 Antibody (A04872-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Figure 2. Flow Cytometry analysis of JK cells using anti-CD84 antibody (A04872-2).
Overlay histogram showing JK cells stained with A04872-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD84 Antibody (A04872-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Click image to see more details
Figure 3. IHC analysis of CD84 using anti-CD84 antibody (A04872-2).
CD84 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CD84 Antibody (A04872-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For CD84 (Source: Uniprot.org, NCBI)
Gene Name
CD84
Full Name
SLAM family member 5
Weight
35883 MW
Alternative Names
SLAM family member 5; Cell surface antigen MAX.3; Hly9-beta; Leukocyte differentiation antigen CD84; Signaling lymphocytic activation molecule 5; CD84; SLAMF5 CD84 LY9B, SLAMF5, hCD84, mCD84 CD84 molecule SLAM family member 5|CD84 (leukocyte )|cell surface MAX.3|hly9-beta|leucocyte differentiation CD84|leukocyte CD84|leukocyte differentiation CD84|signaling lymphocytic activation molecule 5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD84, check out the CD84 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD84: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD84 Antibody (A04872-2)
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Customer Q&As
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13 Customer Q&As for Anti-CD84 Antibody
Question
I see that the anti-CD84 antibody A04872-2 works with ICC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-03-06
Answer
You can find protocols for ICC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-03-06
Question
I have attached the WB image, lot number and protocol we used for visceral pleura using anti-CD84 antibody A04872-2. Please let me know if you require anything else.
J. Brown
Verified customer
Asked: 2019-08-30
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-30
Question
I was wanting to use your anti-CD84 antibody for ICC for human visceral pleura on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human visceral pleura identification?
Verified Customer
Verified customer
Asked: 2019-06-07
Answer
As indicated on the product datasheet, A04872-2 anti-CD84 antibody has been tested for ELISA, Flow Cytometry, IHC, ICC on human tissues. We have an innovator award program that if you test this antibody and show it works in human visceral pleura in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-07
Question
Will anti-CD84 antibody A04872-2 work on pig ELISA with visceral pleura?
Verified Customer
Verified customer
Asked: 2019-04-25
Answer
Our lab technicians have not tested anti-CD84 antibody A04872-2 on pig. You can run a BLAST between pig and the immunogen sequence of anti-CD84 antibody A04872-2 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig visceral pleura in ELISA, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-04-25
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for visceral pleura using anti-CD84 antibody A04872-2. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-01-18
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-01-18
Question
Do you have a BSA free version of anti-CD84 antibody A04872-2 available?
Verified Customer
Verified customer
Asked: 2018-07-17
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-CD84 antibody A04872-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-07-17
Question
Can you help my question with product A04872-2, anti-CD84 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-12-12
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04872-2 anti-CD84 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-12-12
Question
We are currently using anti-CD84 antibody A04872-2 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2017-11-30
Answer
The anti-CD84 antibody (A04872-2) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-11-30
Question
Is a blocking peptide available for product anti-CD84 antibody (A04872-2)?
K. Johnson
Verified customer
Asked: 2017-11-21
Answer
We do provide the blocking peptide for product anti-CD84 antibody (A04872-2). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-11-21
Question
Is this A04872-2 anti-CD84 antibody reactive to the isotypes of CD84?
A. Collins
Verified customer
Asked: 2016-07-19
Answer
The immunogen of A04872-2 anti-CD84 antibody is E. coli-derived human CD84 recombinant protein (Position: K22-G225). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-07-19
Question
Will anti-CD84 antibody A04872-2 work for ICC with visceral pleura?
J. Wu
Verified customer
Asked: 2016-05-26
Answer
According to the expression profile of visceral pleura, CD84 is highly expressed in visceral pleura. So, it is likely that anti-CD84 antibody A04872-2 will work for ICC with visceral pleura.
Boster Scientific Support
Answered: 2016-05-26
Question
I am interested in to test anti-CD84 antibody A04872-2 on human visceral pleura for research purposes, then I may be interested in using anti-CD84 antibody A04872-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
D. Singh
Verified customer
Asked: 2015-04-01
Answer
The products we sell, including anti-CD84 antibody A04872-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-04-01
Question
Would A04872-2 anti-CD84 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
F. Carter
Verified customer
Asked: 2015-02-12
Answer
As indicated on the product datasheet, A04872-2 anti-CD84 antibody as been tested on ICC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-02-12
