Anti-CD8 CD8A Rabbit Monoclonal Antibody

CD8 antibody

Boster Bio Anti-CD8 CD8A Rabbit Monoclonal Antibody catalog # M02236-1. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Rat. Cited in 2 publication(s).

Product Info Summary

SKU: M02236-1
Size: 100 μl
Reactive Species: Human, Rat
Host: Rabbit
Application: IF, IHC, ICC, WB

Product Name

Anti-CD8 CD8A Rabbit Monoclonal Antibody

View all CD8 Antibodies

SKU/Catalog Number

M02236-1

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-CD8 CD8A Rabbit Monoclonal Antibody catalog # M02236-1. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CD8 CD8A Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02236-1)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

EAF-3

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human CD8

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M02236-1 is reactive to CD8A in Human, Rat

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Observed Molecular Weight

36 kDa

Calculated molecular weight

25729 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

M02236-1 is guaranteed for IF, IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200

Positive Control

IHC: Rat kidney tissue, Human glioblastoma tissue, Human kidney tissue, Mouse skin tissue, human colon tissue

Validation Images & Assay Conditions

Gene/Protein Information For CD8A (Source: Uniprot.org, NCBI)

Gene Name

CD8A

Full Name

T-cell surface glycoprotein CD8 alpha chain

Weight

25729 MW

Alternative Names

T-cell surface glycoprotein CD8 alpha chain;T-lymphocyte differentiation antigen T8/Leu-2;CD8a;CD8A;MAL; CD8A CD8, Leu2, p32 CD8a molecule T-cell surface glycoprotein CD8 alpha chain|CD8 , alpha polypeptide (p32)|Leu2 T-lymphocyte |OKT8 T-cell |T cell co-receptor|T-cell Leu2|T-lymphocyte differentiation T8/Leu-2|T8 T-cell

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CD8A, check out the CD8A Infographic

CD8A infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CD8A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M02236-1 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Effect of Hejie decoction on T cell immune state of chronic hepatitis B patients

Xu Y, Tan X, Wang D, Wang W, Li Y, Wu M, Chen S, Wu Y, Tan C. Int J Mol Med. 2015 Jun;35(6):1501-10. Doi: 10.3892/Ijmm.2015.2154. Epub 2015 Mar 24. Elevated Survivin Expression In Peripheral Blood Mononuclear Cells Is Central To Collateral Formati...

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16 Customer Q&As for Anti-CD8 CD8A Rabbit Monoclonal Antibody

Question

I am looking for to test anti-CD8 Rabbit Monoclonal antibody M02236-1 on human thymus for research purposes, then I may be interested in using anti-CD8 Rabbit Monoclonal antibody M02236-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-04-28

Answer

The products we sell, including anti-CD8 Rabbit Monoclonal antibody M02236-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-04-28

Question

I have attached the WB image, lot number and protocol we used for thymus using anti-CD8 Rabbit Monoclonal antibody M02236-1. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-03-06

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-03-06

Question

Is this M02236-1 anti-CD8 Rabbit Monoclonal antibody reactive to the isotypes of CD8A?

Verified Customer

Verified customer

Asked: 2020-02-10

Answer

The immunogen of M02236-1 anti-CD8 Rabbit Monoclonal antibody is A synthesized peptide derived from human CD8. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-02-10

Question

I have a question about product M02236-1, anti-CD8 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-01-27

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M02236-1 anti-CD8 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-01-27

Question

We want using your anti-CD8 Rabbit Monoclonal antibody for transmembrane receptor protein tyrosine kinase signaling pathway studies. Has this antibody been tested with western blotting on jurkat cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-08-13

Answer

We appreciate your inquiry. This M02236-1 anti-CD8 Rabbit Monoclonal antibody is tested on jurkat cell lysate. It is guaranteed to work for IF, IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-08-13

Question

Is there a BSA free version of anti-CD8 Rabbit Monoclonal antibody M02236-1 available?

Verified Customer

Verified customer

Asked: 2019-08-12

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-CD8 Rabbit Monoclonal antibody M02236-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-08-12

Question

Does M02236-1 anti-CD8 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-06-10

Answer

You can see on the product datasheet, M02236-1 anti-CD8 Rabbit Monoclonal antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-06-10

Question

Would anti-CD8 Rabbit Monoclonal antibody M02236-1 work for IF with thymus?

Verified Customer

Verified customer

Asked: 2019-03-26

Answer

According to the expression profile of thymus, CD8A is highly expressed in thymus. So, it is likely that anti-CD8 Rabbit Monoclonal antibody M02236-1 will work for IF with thymus.

Boster Scientific Support

Answered: 2019-03-26

Question

Our team were satisfied with the WB result of your anti-CD8 Rabbit Monoclonal antibody. However we have seen positive staining in pericardium isoform 2: secreted using this antibody. Is that expected? Could you tell me where is CD8A supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-02-26

Answer

From literature, pericardium does express CD8A. Generally CD8A expresses in isoform 1: cell membrane, isoform 2: secreted. Regarding which tissues have CD8A expression, here are a few articles citing expression in various tissues:
Pericardium, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2019-02-26

Question

Is a blocking peptide available for product anti-CD8 Rabbit Monoclonal antibody (M02236-1)?

O. Brown

Verified customer

Asked: 2018-08-22

Answer

We do provide the blocking peptide for product anti-CD8 Rabbit Monoclonal antibody (M02236-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-08-22

Question

I see that the anti-CD8 Rabbit Monoclonal antibody M02236-1 works with IF, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2017-12-28

Answer

You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2017-12-28

Question

We have seen staining in human pericardium. Are there any suggestions? Is anti-CD8 Rabbit Monoclonal antibody supposed to stain pericardium positively?

Verified Customer

Verified customer

Asked: 2017-12-22

Answer

From what I have seen in literature pericardium does express CD8A. From what I have seen in Uniprot.org, CD8A is expressed in thymus, pericardium, among other tissues. Regarding which tissues have CD8A expression, here are a few articles citing expression in various tissues:
Pericardium, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2017-12-22

Question

We are currently using anti-CD8 Rabbit Monoclonal antibody M02236-1 for human tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on dog tissues as well?

D. Krishna

Verified customer

Asked: 2017-10-09

Answer

The anti-CD8 Rabbit Monoclonal antibody (M02236-1) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-10-09

Question

I was wanting to use your anti-CD8 Rabbit Monoclonal antibody for IF for human thymus on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human thymus identification?

E. Mangal

Verified customer

Asked: 2014-08-12

Answer

You can see on the product datasheet, M02236-1 anti-CD8 Rabbit Monoclonal antibody has been validated for IF, IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human thymus in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2014-08-12

Question

We ordered your anti-CD8 Rabbit Monoclonal antibody for WB on pericardium in the past. I am using human, and We are going to use the antibody for IHC next. I was wanting to use examining pericardium as well as thymus in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?

C. Johnson

Verified customer

Asked: 2013-08-30

Answer

I took a look at the website and datasheets of our anti-CD8 Rabbit Monoclonal antibody and it appears that M02236-1 has been validated on human in both WB and IHC. Thus M02236-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2013-08-30

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for thymus using anti-CD8 Rabbit Monoclonal antibody M02236-1. Let me know if you need anything else.

A. Wu

Verified customer

Asked: 2013-03-07

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2013-03-07

Order DetailsPrice
M02236-1-M02236-1

100μL

$370
M02236-1-30ul

30μL sample

$99
M02236-1-carrier-free-1ml

Carrier Free

$2590

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M02236-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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