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Product Info Summary
| SKU: | M00142-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Bcl-6 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00142-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Bcl-6 Rabbit Monoclonal Antibody catalog # M00142-1. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Bcl-6 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00142-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
BAF-2
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Bcl6
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00142-1 is reactive to BCL6 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
95 kDa
Calculated molecular weight
78846 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00142-1 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
Positive Control
WB: human Daudi whole cell, human Raji whole cell
IHC: human tonsil tissue
ICC/IF: Ramos cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of BCL6 using anti-BCL6 antibody (M00142-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Daudi whole cell lysates,
Lane 2: human Raji whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCL6 antigen affinity purified monoclonal antibody (Catalog # M00142-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BCL6 at approximately 95 kDa. The expected band size for BCL6 is at 79 kDa.
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Immunohistochemical analysis of paraffin-embedded human tonsil, using Bcl-6 Antibody.
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Immunofluorescent analysis of Ramos cells, using Bcl-6 Antibody .
Protein Target Info & Infographic
Gene/Protein Information For BCL6 (Source: Uniprot.org, NCBI)
Gene Name
BCL6
Full Name
B-cell lymphoma 6 protein
Weight
78846 MW
Alternative Names
B-cell lymphoma 6 protein;BCL-6;B-cell lymphoma 5 protein;BCL-5;Protein LAZ-3;Zinc finger and BTB domain-containing protein 27;Zinc finger protein 51;BCL6;BCL5, LAZ3, ZBTB27, ZNF51; BCL6 BCL5A, LAZ3, ZBTB27, ZNF51, BCL6 BCL6 transcription repressor B-cell lymphoma 6 protein|B cell CLL/lymphoma 6|B-cell lymphoma 5 protein|B-cell lymphoma 6 protein transcript|BCL-5|BCL-6|cys-his2 zinc finger transcription factor|lymphoma-associated zinc finger gene on chromosome 3|protein LAZ-3|zinc finger and BTB domain-containing protein 27|zinc finger protein 51|zinc finger transcription factor BCL6S
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BCL6, check out the BCL6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BCL6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Bcl-6 Rabbit Monoclonal Antibody (M00142-1)
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Customer Q&As
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16 Customer Q&As for Anti-Bcl-6 Rabbit Monoclonal Antibody
Question
Can you help my question with product M00142-1, anti-Bcl-6 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-02-19
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00142-1 anti-Bcl-6 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-02-19
Question
Is this M00142-1 anti-Bcl-6 Rabbit Monoclonal antibody reactive to the isotypes of BCL6?
Verified Customer
Verified customer
Asked: 2020-01-24
Answer
The immunogen of M00142-1 anti-Bcl-6 Rabbit Monoclonal antibody is A synthesized peptide derived from human Bcl6. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-24
Question
Is a blocking peptide available for product anti-Bcl-6 Rabbit Monoclonal antibody (M00142-1)?
Verified Customer
Verified customer
Asked: 2019-11-20
Answer
We do provide the blocking peptide for product anti-Bcl-6 Rabbit Monoclonal antibody (M00142-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-11-20
Question
I was wanting to use your anti-Bcl-6 Rabbit Monoclonal antibody for IP for human cervix carcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human cervix carcinoma identification?
Verified Customer
Verified customer
Asked: 2019-10-16
Answer
As indicated on the product datasheet, M00142-1 anti-Bcl-6 Rabbit Monoclonal antibody has been tested for IP, IF, IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-16
Question
Would M00142-1 anti-Bcl-6 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-10-10
Answer
It shows on the product datasheet, M00142-1 anti-Bcl-6 Rabbit Monoclonal antibody as been tested on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-10
Question
We are currently using anti-Bcl-6 Rabbit Monoclonal antibody M00142-1 for human tissue, and we are satisfied with the IP results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2018-10-01
Answer
The anti-Bcl-6 Rabbit Monoclonal antibody (M00142-1) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-10-01
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-Bcl-6 Rabbit Monoclonal antibody M00142-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-07-13
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-07-13
Question
Here is the WB image, lot number and protocol we used for cervix carcinoma using anti-Bcl-6 Rabbit Monoclonal antibody M00142-1. Please let me know if you require anything else.
S. Mangal
Verified customer
Asked: 2018-03-05
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-03-05
Question
We purchased anti-Bcl-6 Rabbit Monoclonal antibody for WB on urinary bladder last year. I am using human, and We are going to use the antibody for IHC next. I was wanting to use examining urinary bladder as well as skeletal muscle in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2017-12-27
Answer
I have checked the website and datasheets of our anti-Bcl-6 Rabbit Monoclonal antibody and I see that M00142-1 has been validated on human in both WB and IHC. Thus M00142-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-12-27
Question
I see that the anti-Bcl-6 Rabbit Monoclonal antibody M00142-1 works with IP, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-09-04
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-09-04
Question
Do you have a BSA free version of anti-Bcl-6 Rabbit Monoclonal antibody M00142-1 available?
Verified Customer
Verified customer
Asked: 2017-05-18
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Bcl-6 Rabbit Monoclonal antibody M00142-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-05-18
Question
We have observed staining in human liver. Are there any suggestions? Is anti-Bcl-6 Rabbit Monoclonal antibody supposed to stain liver positively?
A. Krishna
Verified customer
Asked: 2017-05-16
Answer
From what I have seen in literature liver does express BCL6. From what I have seen in Uniprot.org, BCL6 is expressed in urinary bladder, skeletal muscle, liver, cervix carcinoma, among other tissues. Regarding which tissues have BCL6 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 23186163
Liver, Pubmed ID: 8274740, 8506375
Skeletal muscle, Pubmed ID: 8220427
Boster Scientific Support
Answered: 2017-05-16
Question
Would anti-Bcl-6 Rabbit Monoclonal antibody M00142-1 work for IP with cervix carcinoma?
K. Miller
Verified customer
Asked: 2016-12-01
Answer
According to the expression profile of cervix carcinoma, BCL6 is highly expressed in cervix carcinoma. So, it is likely that anti-Bcl-6 Rabbit Monoclonal antibody M00142-1 will work for IP with cervix carcinoma.
Boster Scientific Support
Answered: 2016-12-01
Question
Our lab were satisfied with the WB result of your anti-Bcl-6 Rabbit Monoclonal antibody. However we have been able to see positive staining in urinary bladder nucleus using this antibody. Is that expected? Could you tell me where is BCL6 supposed to be expressed?
H. Baker
Verified customer
Asked: 2015-12-31
Answer
Based on literature, urinary bladder does express BCL6. Generally BCL6 expresses in nucleus. Regarding which tissues have BCL6 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 23186163
Liver, Pubmed ID: 8274740, 8506375
Skeletal muscle, Pubmed ID: 8220427
Boster Scientific Support
Answered: 2015-12-31
Question
Would anti-Bcl-6 Rabbit Monoclonal antibody M00142-1 work on pig IP with liver?
K. Miller
Verified customer
Asked: 2015-05-06
Answer
Our lab technicians have not tested anti-Bcl-6 Rabbit Monoclonal antibody M00142-1 on pig. You can run a BLAST between pig and the immunogen sequence of anti-Bcl-6 Rabbit Monoclonal antibody M00142-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig liver in IP, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-05-06
Question
Our lab want to know about to test anti-Bcl-6 Rabbit Monoclonal antibody M00142-1 on human cervix carcinoma for research purposes, then I may be interested in using anti-Bcl-6 Rabbit Monoclonal antibody M00142-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
W. Parker
Verified customer
Asked: 2014-03-18
Answer
The products we sell, including anti-Bcl-6 Rabbit Monoclonal antibody M00142-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-03-18
