Anti-CD74 Monoclonal Antibody

Product Name

Anti-CD74 Monoclonal Antibody

View all CD74 Antibodies

SKU/Catalog Number

M01340

Size

100ug

Form

liquid

Description

Boster Bio Anti-CD74 Monoclonal Antibody catalog # M01340. Tested in Flow Cytometry, IP, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CD74 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01340)

Host

Mouse

Contents

PBS pH7.2, 50% glycerol, 0.09% sodium azide

Clonality

Monoclonal

Clone Number

PIN.1

Isotype

IgG1

Immunogen

Human CD74 invariant chain synthetic peptide

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~33-35kDa protein doublet corresponding to the molecular mass of the p33 and p35 forms of human CD74.

Reactive Species

M01340 is reactive to CD74 in Human, Mouse

Reconstitution

Observed Molecular Weight

42 kDa

Calculated molecular weight

95797 MW

Background of CD74

CD74 is a non-polymorphic type II integral membrane protein. It has a short N-terminal cytoplasmic tail of 28 amino acids, followed by a single 24-aa transmembrane region and an approximately 150-aa lumenal domain (1). The CD74 chain is thought to function mainly as an MHC class II chaperone, which promotes ER exit of MHC class II molecules, directs them to endocytic compartments, prevents peptide binding in the ER, and contributes to peptide editing in the MHC class II compartment. Class II MHC and Ii expression was believed to be restricted to classical antigen-presenting cells (APC); however, during inflammation, other cell types, including mucosal epithelial cells, have also been reported to express class II MHC molecules (2). Experiments that investigate cell-surface CD74 are complicated by the fact that CD74 remains on the cell surface for a very short time. The surface half-life of CD74 was calculated to be fewer than 10 minutes (3). CD74 however has also recently been shown to have a role as an accessory-signaling molecule because of its high-affinity binding to the pro-inflammatory cytokine, macrophage migration-inhibitory factor (MIF) (3). The restricted expression of CD74 by normal tissues and its very rapid internalization make CD74 an attractive therapeutic target for both cancer and immunologic diseases (4).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M01340 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB, FACS Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB (1:1000), IHC (1:100), ICC/IF (1:50); optimal dilutions for assays should be determined by the user.

Validation Images & Assay Conditions

Gene/Protein Information For CD74 (Source: Uniprot.org, NCBI)

Alternative Names

DHLAG, HLA DR gamma, HLADG, p33, p35, Protein 41, CD 74, CD74, CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated), CD74 antigen, CD74 molecule, CD74 molecule, major histocompatibility complex, class II invariant chain, CLIP, DHLAG, Gamma chain of class II antigens, HG2A_HUMAN, HLA class II histocompatibility antigen gamma chain, HLA DR antigens associated invariant chain, HLA DR gamma, HLA-DR antigens-associated invariant chain, HLA-DR-gamma, HLADG, HLADR antigens associated invariant chain Ia antigen associated invariant chain, Ia antigen-associated invariant chain, Ia associated invariant chain, Ia gamma, Ii, Invariant polypeptide of major histocompatibility complex class II antigen associated, la-gamma, Major histocompatibility complex class II invariant chain, MHC HLA DR gamma chain, MHC HLA-DR gamma chain, p33, p35, Protein 41 CD74 DHLAG, HLADG, II, Ia-GAMMA, p33 CD74 molecule HLA class II histocompatibility gamma chain|CD74 (invariant polypeptide of major histocompatibility complex, class II -associated)|CD74 molecule, major histocompatibility complex, class II invariant chain|HLA-DR s-associated invariant chain|HLA-DR-gamma|Ia-associated invariant chain|MHC HLA-DR gamma chain|gamma chain of class II s

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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