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Product Info Summary
| SKU: | A13573 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-BRI3B BRI3BP Antibody
SKU/Catalog Number
A13573
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-BRI3B BRI3BP Antibody (Catalog # A13573). Tested in WB, IHC applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-BRI3B BRI3BP Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13573)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human BRI3B.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A13573 is reactive to BRI3BP in Human, Mouse
Reconstitution
Calculated molecular weight
27836 MW
Background of Bri3bp
Involved in tumorigenesis and may function by stabilizing p53/TP53.
Lin L., Biochem. Genet. 39:369-377(2001).
The MGC Project Team, Genome Res. 14:2121-2127(2004).
Kim J.W., Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A13573 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
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Western blot analysis of extracts from RAW264.7 cells, using BRI3B antibody A13573.
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Immunohistochemistry analysis of paraffin-embedded human brain tissue using BRI3B antibody A13573.
Protein Target Info & Infographic
Gene/Protein Information For BRI3BP (Source: Uniprot.org, NCBI)
Gene Name
BRI3BP
Full Name
BRI3-binding protein
Weight
27836 MW
Alternative Names
BRI3-binding protein;I3-binding protein;Cervical cancer 1 proto-oncogene-binding protein KG19;HCCRBP-1;BRI3BP ;KG19 ;
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BRI3BP, check out the BRI3BP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BRI3BP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-BRI3B BRI3BP Antibody (A13573)
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Customer Q&As
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6 Customer Q&As for Anti-BRI3B BRI3BP Antibody
Question
I see that the anti-BRI3B antibody A13573 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-01-23
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-01-23
Question
Is a blocking peptide available for product anti-BRI3B antibody (A13573)?
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
We do provide the blocking peptide for product anti-BRI3B antibody (A13573). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-12-13
Question
Does A13573 anti-BRI3B antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-09-30
Answer
It shows on the product datasheet, A13573 anti-BRI3B antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-30
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for skin {eco:0000312 using anti-BRI3B antibody A13573. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-09-27
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-27
Question
See attached the WB image, lot number and protocol we used for skin {eco:0000312 using anti-BRI3B antibody A13573. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-10-06
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-10-06
Question
My question regarding product A13573, anti-BRI3B antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
B. Patel
Verified customer
Asked: 2017-01-10
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A13573 anti-BRI3B antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-01-10
