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- Table of Contents
1 Citations 3 Q&As
Facts about CD63 antigen.
Plays a role in VEGFA signaling via its role in regulating the internalization of KDR/VEGFR2. Plays a role in intracellular vesicular transport processes, and is necessary for normal trafficking of the PMEL luminal domain that is essential for the growth and maturation of melanocytes.
Human | |
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Gene Name: | CD63 |
Uniprot: | P08962 |
Entrez: | 967 |
Belongs to: |
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tetraspanin (TM4SF) family |
CD63 antigen (melanoma 1 antigen); CD63 antigen; CD63 molecule; CD63; Granulophysin; Lamp-3; Lysosomal-associated membrane protein 3; ME491; melanoma 1 antigen; Melanoma-associated antigen ME491; melanoma-associated antigen MLA1; MLA1lysosome-associated membrane glycoprotein 3; Ocular melanoma-associated antigen; OMA81H; tetraspanin-30; Tspan30; tspan-30; TSPAN30granulophysin
Mass (kDA):
25.637 kDA
Human | |
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Location: | 12q13.2 |
Sequence: | 12; NC_000012.12 (55725323..55729673, complement) |
Detected in platelets (at protein level). Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages.
Cell membrane; Multi-pass membrane protein. Lysosome membrane; Multi-pass membrane protein. Late endosome membrane; Multi-pass membrane protein. Endosome, multivesicular body. Melanosome. Secreted, extracellular exosome. Cell surface. Also found in Weibel-Palade bodies of endothelial cells (PubMed:10793155). Located in platelet dense granules (PubMed:7682577). Detected in a subset of pre-melanosomes. Detected on intralumenal vesicles (ILVs) within multivesicular bodies (PubMed:21962903).
PMID: 3365686 by Hotta H., et al. Molecular cloning and characterization of an antigen associated with early stages of melanoma tumor progression.
PMID: 2171551 by Rapp G., et al. Characterization of three abundant mRNAs from human ovarian granulosa cells.
CD63 Antibody is a monoclonal antibody that has been produced against CD63. It is used for isotype control. The primary and secondary antibodies are mouse IgG and phycoerythrin-conjugated. The antibody was made from PBMCs from human. The PBMCs were fixed in paraformaldehyde before being permeabilized with saponin. Then the cells were incubated in the dispersed anti-human CD63 Monoclonal antibody.
The CD63 antibody recognizes the protein that is on the cell's surface. It is expressed on the membrane of the exosome and is activated in the normal course of cell development. It is also expressed on the cell surface by monocytes and platelets. In humans, it is present on the surface of granulocytes and macrophages, and is weakly expressed in granulocytes. The antigen is the same as the antigen that causes melanoma ME491 and the platelet antigen PTLGP40. Abcam has been a leader in reproducibility and has knockout cell lines as well as gold standard validation.
There are some caveats to using an anti-CD63 antibody in clinical settings, the most significant is patient compliance. The CD63 BAT can be used to identify the presence of autoimmune CSU patients. While it is not as sensitive as an IgG however, it is much more sensitive and specific than monoclonal antibodies which are ineffective in patients suffering from inflammatory intestinal disease or Psoriasis.
The CD63 antibody, which is a tetraspanin, is a member of the LAMP-3 superfamily. It is extensively expressed in the extracellular space , and is found on the plasma membrane following activation. It is important to be aware that interactions between CD63-adaptor proteins regulate cell activity. The 67% amino acid sequence identity of the human CD63 anti-rat CD63 and mouse CD63 is shared by both species. Although the functional profiles of the two species are identical but their clinical significance is unknown.
The CD63 Antibody monitors the disease's activity and response. It gives information about the CSU and the treatment it offers. Different studies have proven that the CD63 antibody is a powerful method of monitoring the activity of the disease. This way the CD63 Antibody can provide useful information. The immune system is largely affected by the level in the blood of CD63. CD63 is extremely sensitive to antibodies. It is essential to stay clear of negative side effects from this medication.
The CD63 antibody, a non-specific anti-inflammatory antibodythat detects the presence of apoptotic cells inside the body of a cell. It is an important part of the immune system. It is essential to maintain the health of the cell. It could indicate the presence or absence of apoptotic cells. In addition, it could aid in determining the severity of inflammation. The antibody can also boost the immune system's ability to fight.
The CD63 level is associated with the Atopy. The high level of CD63 means higher disease activity. In a study involving pediatric patients and their parents, the CD63 BAT levels were lower than those of non-atopic patients. The number of lymphocytes found in the lung is linked to CD63 levels. They were also related to disease activity based on the UAS7 score. The high antibody level suggests that aphluorin was present in the immune system.
The aim of the study was to establish a reference range for the CD63 BAT results that are based on expression for children with no CSU. The aim of the study was to evaluate the impact of gender, age, ethnicity and disease activity on the BAT results. The researchers concluded that the high expression levels of the patient population were significantly different from those of the controls. This is statistically significant but the Wilcoxon rank sum difference was less than 0.23 for controls and patients with PU.
In a recent study 23 patients in the pediatric population with PU were tested for the expression of CD63. The majority of the patients were males and had an IgE of 947 mg/L. The median CD63 levels in patients was 1.88 percent and is similar to the median value in a study with healthy subjects. In comparison to the controls, the BAT values did differ statistically between PU patients and the controls. The Wilcoxon rank sum difference was 840 and p = 0.23 respectively.
25682-1-AP, a human CD63 antibody was utilized to test the CD63 protein in CSU patients. To determine the amount of CD63 expression in human patients the anti-CD63 antibody (25682-1-AP) was employed. The dilution of the anti-CD63 was 1:800, and the samples were examined using an 80x lens, and incubated overnight at 4 degrees Celsius. The CD63 dilution in the control cells was similar to the value of the reference samples.
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