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Anti-CD59/Cd59a Antibody Picoband®

Name: Anti-CD59/Cd59a Antibody Picoband®
Brand: Boster Bio
SKU: A00914
Price: 370 USD
Availability: InStock
Rating: 100 (3 reviews)

Cd59a antibody

Boster Bio Anti-CD59/Cd59a Antibody Picoband® catalog # A00914. Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU:	A00914
Size:	100 μg/vial
Reactive Species:	Mouse, Rat
Host:	Rabbit
Application:	ELISA, IHC, WB

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Product info

Product Name

Anti-CD59/Cd59a Antibody Picoband®

View all Cd59a Antibodies

SKU/Catalog Number

A00914

Size

100 μg/vial

Form

Lyophilized

Description

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CD59/Cd59a Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00914)

Host

Rabbit

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived mouse CD59 recombinant protein (Position: L24-S96).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00914 is reactive to Cd59a in Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

20 kDa

Calculated molecular weight

13.648kDa

Background of Cd59a

CD59 glycoprotein, also known as MAC-inhibitory protein (MAC-IP), membrane inhibitor of reactive lysis (MIRL), or protectin, is a protein that in humans is encoded by the CD59 gene. This gene encodes a cell surface glycoprotein that regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. This protein also plays a role in signal transduction pathways in the activation of T cells. Mutations in this gene cause CD59 deficiency, a disease resulting in hemolytic anemia and thrombosis, and which causes cerebral infarction. Multiple alternatively spliced transcript variants, which encode the same protein, have been identified for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Application & Images

Applications

A00914 is guaranteed for ELISA, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
ELISA, 0.1-0.5μg/ml

Positive Control

WB: rat liver tissue, rat kidney tissue, rat spleen tissue, rat brain tissue
IHC: mouse spleen tissue, mouse thymus tissue

Validation Images & Assay Conditions

a00914 cd59 primary antibodies wb testing 1

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Figure 1. Western blot analysis of CD59 using anti-CD59 antibody (A00914).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: rat kidney tissue lysates,
Lane 3: rat spleen tissue lysates,
Lane 4: rat brain tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD59 antigen affinity purified polyclonal antibody (Catalog # A00914) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD59 at approximately 20KD. The expected band size for CD59 is at 14KD.

A00914 CD59 primary antibodies IHC testing 2

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Figure 2. IHC analysis of CD59 using anti-CD59 antibody (A00914).
CD59 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CD59 Antibody (A00914) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

A00914 CD59 primary antibodies IHC testing 3

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Figure 3. IHC analysis of CD59 using anti-CD59 antibody (A00914).
CD59 was detected in paraffin-embedded section of mouse thymus tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CD59 Antibody (A00914) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Protein Target Info & Infographic

Gene/Protein Information For Cd59a (Source: Uniprot.org, NCBI)

Gene Name

Cd59a

Full Name

CD59A glycoprotein

Weight

13.648kDa

Alternative Names

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Cd59a, check out the Cd59a Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Cd59a: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the Cd59a Infographic

Specific Publications For Anti-CD59/Cd59a Antibody Picoband® (A00914)

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Boster Scientific Support

Answered: 2014-04-18

Order Details		Price
	A00914 100μg	$370
	A00914-10ug 10μg sample (liquid)	$99
	A00914-Biotin 100 μg Biotin conjugated	$570
	A00914-Cy3 100 μg Cy3 conjugated	$570
	A00914-Dylight488 100 μg Dylight488 conjugated	$570
	A00914-Dylight550 100 μg Dylight550 conjugated	$570
	A00914-Dylight594 100 μg Dylight594 conjugated	$570
	A00914-FITC 100 μg FITC conjugated	$570
	A00914-HRP 100 μg HRP conjugated	$570
	A00914-APC 100 μg APC conjugated	$670
	A00914-PE 100 μg PE conjugated	$670
	A00914-iFluor647 100 μg iFluor647 conjugated	$670
	A00914-carrier-free Carrier Free	$370

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A00914

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)

$370.00

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Anti-CD59/Cd59a Antibody Picoband®

Cd59a antibody