Anti-CD59/Cd59a Antibody Picoband®

Cd59a antibody

Boster Bio Anti-CD59/Cd59a Antibody Picoband® catalog # A00914. Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: A00914
Size: 100 μg/vial
Reactive Species: Mouse, Rat
Host: Rabbit
Application: ELISA, IHC, WB

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Product Name

Anti-CD59/Cd59a Antibody Picoband®

View all Cd59a Antibodies

SKU/Catalog Number

A00914

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CD59/Cd59a Antibody Picoband® catalog # A00914. Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CD59/Cd59a Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00914)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived mouse CD59 recombinant protein (Position: L24-S96).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00914 is reactive to Cd59a in Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

20 kDa

Calculated molecular weight

13.648kDa

Background of Cd59a

CD59 glycoprotein, also known as MAC-inhibitory protein (MAC-IP), membrane inhibitor of reactive lysis (MIRL), or protectin, is a protein that in humans is encoded by the CD59 gene. This gene encodes a cell surface glycoprotein that regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. This protein also plays a role in signal transduction pathways in the activation of T cells. Mutations in this gene cause CD59 deficiency, a disease resulting in hemolytic anemia and thrombosis, and which causes cerebral infarction. Multiple alternatively spliced transcript variants, which encode the same protein, have been identified for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A00914 is guaranteed for ELISA, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
ELISA, 0.1-0.5μg/ml

Positive Control

WB: rat liver tissue, rat kidney tissue, rat spleen tissue, rat brain tissue
IHC: mouse spleen tissue, mouse thymus tissue

Validation Images & Assay Conditions

Gene/Protein Information For Cd59a (Source: Uniprot.org, NCBI)

Gene Name

Cd59a

Full Name

CD59A glycoprotein

Weight

13.648kDa

Alternative Names

CD59A glycoprotein; MAC-inhibitory protein; MAC-IP; Membrane attack complex inhibition factor; MACIF; Protectin; CD59; Cd59a; Cd59 Cd59a|AA987121, Cd59, prot, protectin|CD59a |CD59A glycoprotein|CD59 |MAC-IP|MAC-inhibitory protein|MACIF|membrane attack complex inhibition factor

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Cd59a, check out the Cd59a Infographic

Cd59a infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Cd59a: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-CD59/Cd59a Antibody Picoband®

Question

We are currently using anti-CD59/Cd59a antibody A00914 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it possible that the antibody can work on goat tissues as well?

Verified Customer

Verified customer

Asked: 2019-11-04

Answer

The anti-CD59/Cd59a antibody (A00914) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-11-04

Question

Does A00914 anti-CD59/Cd59a antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-10-07

Answer

It shows on the product datasheet, A00914 anti-CD59/Cd59a antibody as been validated on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-10-07

Question

Is there a BSA free version of anti-CD59/Cd59a antibody A00914 available?

Verified Customer

Verified customer

Asked: 2019-05-22

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD59/Cd59a antibody A00914 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-05-22

Question

We want to test anti-CD59/Cd59a antibody A00914 on mouse kidney for research purposes, then I may be interested in using anti-CD59/Cd59a antibody A00914 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-03-27

Answer

The products we sell, including anti-CD59/Cd59a antibody A00914, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-03-27

Question

I see that the anti-CD59/Cd59a antibody A00914 works with ELISA, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2018-01-31

Answer

You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-01-31

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for kidney using anti-CD59/Cd59a antibody A00914. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2017-12-27

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-12-27

Question

Does anti-CD59/Cd59a antibody A00914 work on pig WB with kidney?

T. Evans

Verified customer

Asked: 2014-04-18

Answer

Our lab technicians have not tested anti-CD59/Cd59a antibody A00914 on pig. You can run a BLAST between pig and the immunogen sequence of anti-CD59/Cd59a antibody A00914 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig kidney in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2014-04-18

Order DetailsPrice
A00914

100μg

$370
A00914-10ug

10μg sample (liquid)

$99
A00914-Biotin

100 μg Biotin conjugated

$570
A00914-Cy3

100 μg Cy3 conjugated

$570
A00914-Dylight488

100 μg Dylight488 conjugated

$570
A00914-Dylight550

100 μg Dylight550 conjugated

$570
A00914-Dylight594

100 μg Dylight594 conjugated

$570
A00914-FITC

100 μg FITC conjugated

$570
A00914-HRP

100 μg HRP conjugated

$570
A00914-APC

100 μg APC conjugated

$670
A00914-PE

100 μg PE conjugated

$670
A00914-iFluor647

100 μg iFluor647 conjugated

$670
A00914-carrier-free

Carrier Free

$370

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A00914
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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