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Product Info Summary
| SKU: | M00480-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-CD5 Antibody Picoband® (monoclonal, 4E2)
SKU/Catalog Number
M00480-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD5 Antibody Picoband® (monoclonal, 4E2) catalog # M00480-2. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD5 Antibody Picoband® (monoclonal, 4E2) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00480-2)
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Monoclonal
Clone Number
4E2
Isotype
Mouse IgG1
Immunogen
E. coli-derived human CD5 recombinant protein (Position: R25-L495).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M00480-2 is reactive to CD5 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Observed Molecular Weight
67 kDa
Calculated molecular weight
54.578kDa
Background of CD5
CD5 is a member of the scavenger receptor cysteine-rich (SRCR) superfamily. Members of this family are secreted or membrane-anchored proteins mainly found in cells associated with the immune system. In humans, the gene is located on the long arm of chromosome 11. This protein is a type-I transmembrane glycoprotein found on the surface of thymocytes, T lymphocytes and a subset of B lymphocytes. The encoded protein contains three SRCR domains and may act as a receptor to regulate T-cell proliferation. Alternative splicing results in multiple transcript variants encoding different isoforms.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00480-2 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Positive Control
WB: human Jurkat tissue, human CCRM-CEM whole cell,
IHC: human tonsil cancer tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CD5 using anti-CD5 antibody (M00480-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Jurkat tissue lysates,
Lane 2: human CCRM-CEM whole cell lysates,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CD5 antigen affinity purified polyclonal antibody (Catalog # M00480-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for CD5 at approximately 67KD. The expected band size for CD5 is at 54KD.
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Figure 2. IHC analysis of CD5 using anti-CD5 antibody (M00480-2).
CD5 was detected in paraffin-embedded section of human tonsil cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-CD5 Antibody (M00480-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For CD5 (Source: Uniprot.org, NCBI)
Gene Name
CD5
Full Name
T-cell surface glycoprotein CD5
Weight
54.578kDa
Alternative Names
T-cell surface glycoprotein CD5; Lymphocyte antigen T1/Leu-1; CD5; CD5; LEU1 CD5 LEU1, T1 CD5 molecule T-cell surface glycoprotein CD5|CD5 (p56-62)|epididymis secretory sperm binding protein|lymphocyte T1/Leu-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD5, check out the CD5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD5 Antibody Picoband® (monoclonal, 4E2) (M00480-2)
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Customer Q&As
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4 Customer Q&As for Anti-CD5 Antibody Picoband® (monoclonal, 4E2)
Question
We are currently using anti-CD5 antibody (monoclonal, 4E2) M00480-2 for human tissue, and we are satisfied with the IHC-P results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-22
Answer
The anti-CD5 antibody (monoclonal, 4E2) (M00480-2) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-22
Question
We bought anti-CD5 antibody (monoclonal, 4E2) for IHC-P on thymus a few months ago. I am using human, and We intend to use the antibody for WB next. I am interested in examining thymus as well as leukemic t-cell in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2020-01-10
Answer
I have checked the website and datasheets of our anti-CD5 antibody (monoclonal, 4E2) and it appears that M00480-2 has been tested on human in both IHC-P and WB. Thus M00480-2 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-01-10
Question
Our lab were well pleased with the WB result of your anti-CD5 antibody (monoclonal, 4E2). However we have seen positive staining in pancreas cell membrane using this antibody. Is that expected? Could you tell me where is CD5 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-07-02
Answer
From literature, pancreas does express CD5. Generally CD5 expresses in cell membrane. Regarding which tissues have CD5 expression, here are a few articles citing expression in various tissues:
Leukemic T-cell, Pubmed ID: 15144186, 19690332
Lymphocyte, Pubmed ID: 8740779
Pancreas, Pubmed ID: 15489334
Thymus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-07-02
Question
We have observed staining in human thymus. Any tips? Is anti-CD5 antibody (monoclonal, 4E2) supposed to stain thymus positively?
R. Krishna
Verified customer
Asked: 2013-05-06
Answer
From what I have seen in literature thymus does express CD5. From what I have seen in Uniprot.org, CD5 is expressed in leukocyte, lymphocyte, thymus, pancreas, leukemic t-cell, among other tissues. Regarding which tissues have CD5 expression, here are a few articles citing expression in various tissues:
Leukemic T-cell, Pubmed ID: 15144186, 19690332
Lymphocyte, Pubmed ID: 8740779
Pancreas, Pubmed ID: 15489334
Thymus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2013-05-06
