Product Info Summary
SKU: | A00480-2 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IF, IHC, IHC-F, ICC, WB |
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Product info
Product Name
Anti-CD5 Antibody Picoband®
SKU/Catalog Number
A00480-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD5 Antibody Picoband® catalog # A00480-2. Tested in ELISA, Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00480-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human CD5 recombinant protein (Position: R25-L495).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00480-2 is reactive to CD5 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
67 kDa
Calculated molecular weight
18866 MW
Background of CD5
CD5 is a member of the scavenger receptor cysteine-rich (SRCR) superfamily. Members of this family are secreted or membrane-anchored proteins mainly found in cells associated with the immune system. In humans, the gene is located on the long arm of chromosome 11. This protein is a type-I transmembrane glycoprotein found on the surface of thymocytes, T lymphocytes and a subset of B lymphocytes. The encoded protein contains three SRCR domains and may act as a receptor to regulate T-cell proliferation. Alternative splicing results in multiple transcript variants encoding different isoforms.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00480-2 is guaranteed for ELISA, Flow Cytometry, IF, IHC, IHC-F, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunohistochemistry (Frozen Section), 0.5-1μg/ml
Immunocytochemistry, 0.5-1μg/ml
Immunofluorescence, 2μg/ml
Flow Cytometry (Fixed), 1-3μg/1x106 cells
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human Jurkat whole cell, human CCRF-CEM whole cell, mouse NIH3T3 whole cell
IHC: mouse spleen tissue, human tonsil tissue, rat spleen tissue
IF: human tonsil tissue
FCM: EL-4 cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of CD5 using anti-CD5 antibody (A00480-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human CCRF-CEM whole cell lysates,
Lane 3: mouse NIH3T3 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD5 antigen affinity purified polyclonal antibody (Catalog # A00480-2) at 0.5 μg/mL overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD5 at approximately 67KD. The expected band size for CD5 is at 54KD.
Click image to see more details
Figure 2. IHC analysis of CD5 using anti-CD5 antibody (A00480-2).
CD5 was detected in paraffin-embedded section of mouse spleen tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-CD5 Antibody (A00480-2) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of CD5 using anti-CD5 antibody (A00480-2).
CD5 was detected in paraffin-embedded section of human tonsil tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-CD5 Antibody (A00480-2) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of CD5 using anti-CD5 antibody (A00480-2).
CD5 was detected in paraffin-embedded section of rat spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-CD5 Antibody (A00480-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 5. Flow Cytometry analysis of EL-4 cells using anti-CD5 antibody (A00480-2).
Overlay histogram showing EL-4 cells stained with A00480-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD5 Antibody (A00480-2,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Click image to see more details
Figure 6. IF analysis of CD68 and CD5 using anti-CD68 antibody (M00602) and anti-CD5 antibody (A00480-2)
CD68 and CD5 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL mouse anti-CD68 antibody (M00602) and rabbit anti-CD5 antibody (A00480-2) overnight at 4°C. DyLight488 Conjugated Goat Anti-Mouse IgG (BA1126) and Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The tissue section was developed using Cy3 Conjugated Avidin (BA1037). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For CD5 (Source: Uniprot.org, NCBI)
Gene Name
CD5
Full Name
T-cell surface glycoprotein CD5
Weight
18866 MW
Alternative Names
T-cell surface glycoprotein CD5; Lymphocyte antigen T1/Leu-1; CD5; CD5; LEU1 CD5 LEU1, T1 CD5 molecule T-cell surface glycoprotein CD5|CD5 (p56-62)|epididymis secretory sperm binding protein|lymphocyte T1/Leu-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD5, check out the CD5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD5 Antibody Picoband® (A00480-2)
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Customer Reviews
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1 Reviews For Anti-CD5 Antibody Picoband®
0
Immunohistochemistry for Anti-CD5 Antibody
Excellent
Application | Immunohistochemistry (paraffin-embedded) |
---|---|
Blocking step | 5% BSA as a blocking agent for 30 min at 37°C |
Sample | Human Tonsil |
Fixative | Fixed with 4% paraformaldehyde |
Primary Ab Incubation | 4°C overnight |
Primary Ab Incubation diluent | 5% BSA in TBS |
Primary Ab Concentration | 0.5 ug/ml |
Secondary Antibody | SABC kit from Boster Bio, (SA1022) |
Secondary Ab Dilution | The kit was ready to use, no dilution needed |
Secondary Ab Incubation | at 37°C for 30 min |
W. Sunder
Verified customer
Submitted 2020-05-09
Customer Q&As
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16 Customer Q&As for Anti-CD5 Antibody Picoband®
Question
We were well pleased with the WB result of your anti-CD5 antibody. However we have observed positive staining in leukocyte cell membrane using this antibody. Is that expected? Could you tell me where is CD5 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-05-04
Answer
According to literature, leukocyte does express CD5. Generally CD5 expresses in cell membrane. Regarding which tissues have CD5 expression, here are a few articles citing expression in various tissues:
Leukemic T-cell, Pubmed ID: 15144186, 19690332
Lymphocyte, Pubmed ID: 8740779
Pancreas, Pubmed ID: 15489334
Thymus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2020-05-04
Question
Would A00480-2 anti-CD5 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
N. Carter
Verified customer
Asked: 2020-03-27
Answer
You can see on the product datasheet, A00480-2 anti-CD5 antibody as been validated on IHC-F. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-27
Question
I have a question about product A00480-2, anti-CD5 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-12-30
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00480-2 anti-CD5 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-30
Question
Does anti-CD5 antibody A00480-2 work for IHC-F with thymus?
Verified Customer
Verified customer
Asked: 2019-09-25
Answer
According to the expression profile of thymus, CD5 is highly expressed in thymus. So, it is likely that anti-CD5 antibody A00480-2 will work for IHC-F with thymus.
Boster Scientific Support
Answered: 2019-09-25
Question
Is a blocking peptide available for product anti-CD5 antibody (A00480-2)?
Verified Customer
Verified customer
Asked: 2019-09-10
Answer
We do provide the blocking peptide for product anti-CD5 antibody (A00480-2). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-09-10
Question
I see that the anti-CD5 antibody A00480-2 works with IHC-F, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-08-16
Answer
You can find protocols for IHC-F on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-08-16
Question
I am interested in to test anti-CD5 antibody A00480-2 on mouse thymus for research purposes, then I may be interested in using anti-CD5 antibody A00480-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-06-03
Answer
The products we sell, including anti-CD5 antibody A00480-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-06-03
Question
We want using your anti-CD5 antibody for cell recognition studies. Has this antibody been tested with western blotting on human tonsil tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-05-14
Answer
I appreciate your inquiry. This A00480-2 anti-CD5 antibody is validated on human tonsil tissue, jurkat whole cell lysates, mouse spleen tissue, nih3t3 whole cell lysates. It is guaranteed to work for ELISA, Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-05-14
Question
I was wanting to use your anti-CD5 antibody for IHC-F for mouse thymus on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse thymus identification?
Verified Customer
Verified customer
Asked: 2018-09-11
Answer
As indicated on the product datasheet, A00480-2 anti-CD5 antibody has been validated for ELISA, Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse thymus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-09-11
Question
See below the WB image, lot number and protocol we used for thymus using anti-CD5 antibody A00480-2. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-08-21
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-08-21
Question
We have tried in the past anti-CD5 antibody for IF on thymus in the past. I am using rat, and We intend to use the antibody for ELISA next. I am looking for examining thymus as well as lymphocyte in our next experiment. Could you please give me some suggestion on which antibody would work the best for ELISA?
F. Dhar
Verified customer
Asked: 2018-07-30
Answer
I looked at the website and datasheets of our anti-CD5 antibody and it seems that A00480-2 has been validated on rat in both IF and ELISA. Thus A00480-2 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-07-30
Question
We have seen staining in human leukocyte. What should we do? Is anti-CD5 antibody supposed to stain leukocyte positively?
Verified Customer
Verified customer
Asked: 2018-07-30
Answer
From what I have seen in literature leukocyte does express CD5. From what I have seen in Uniprot.org, CD5 is expressed in leukocyte, lymphocyte, thymus, pancreas, leukemic t-cell, among other tissues. Regarding which tissues have CD5 expression, here are a few articles citing expression in various tissues:
Leukemic T-cell, Pubmed ID: 15144186, 19690332
Lymphocyte, Pubmed ID: 8740779
Pancreas, Pubmed ID: 15489334
Thymus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2018-07-30
Question
We are currently using anti-CD5 antibody A00480-2 for human tissue, and we are content with the IHC-P results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2018-06-22
Answer
The anti-CD5 antibody (A00480-2) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-06-22
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for thymus using anti-CD5 antibody A00480-2. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-01-17
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-01-17
Question
Is this A00480-2 anti-CD5 antibody reactive to the isotypes of CD5?
C. Baker
Verified customer
Asked: 2015-03-19
Answer
The immunogen of A00480-2 anti-CD5 antibody is E. coli-derived human CD5 recombinant protein (Position: R25-L495). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-03-19
Question
Is there a BSA free version of anti-CD5 antibody A00480-2 available?
G. Gonzalez
Verified customer
Asked: 2013-05-13
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-CD5 antibody A00480-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-05-13