Anti-CD5 (Mantle Cell Lymphoma Marker) Monoclonal Antibody

Product Name

Anti-CD5 (Mantle Cell Lymphoma Marker) Monoclonal Antibody

View all CD5 Antibodies

SKU/Catalog Number

M00480

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-CD5 (Mantle Cell Lymphoma Marker) Monoclonal Antibody (Catalog # M00480). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human, Monkey, Horse, Pig, Rabbit, Cow, Rat, Guinea pig, Chicken.

Storage & Handling

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-CD5 (Mantle Cell Lymphoma Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00480)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: CD5/54/F6

Isotype

IgG1, kappa

Immunogen

Recombinant fragment (around aa450-495) of human CD5 protein (intracellular region).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00480 is reactive to CD5 in Chicken, Cow, Guinea pig, Human, Monkey, Pig, Rabbit, Rat, Horse

Reconstitution

Reconstitute with 1ml water (15 minutes at room temperature).

Calculated molecular weight

54578 MW

Background of CD5

Recognizes a 67kDa transmembrane protein, which is identified as CD5. The CD5 antigen is found on 95% of thymocytes and 72% of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. Anti-CD5 is a pan T-cell marker that also reacts with a range of neoplastic B-cells, e.g. chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), mantle cell lymphoma, and a subset (~10%) of diffuse large B-cell lymphoma. CD5 aberrant expression is useful in making a diagnosis of mature T-cell neoplasms. Anti-CD5 detection is diagnostic in CLL/SLL within a panel of other B-cell markers, especially one that includes anti-CD23. Anti-CD5 is also very useful in differentiating among mature small lymphoid cell malignancies. In addition, anti-CD5 can be used in distinguishing thymic carcinoma (+) from thymoma (-). Anti-CD5 does not react with granulocytes or monocytes.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M00480 is guaranteed for Flow Cytometry, IF, IHC Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

Gene/Protein Information For CD5 (Source: Uniprot.org, NCBI)

Alternative Names

T-cell surface glycoprotein CD5;Lymphocyte antigen T1/Leu-1;CD5;CD5;LEU1; CD5 LEU1, T1 CD5 molecule T-cell surface glycoprotein CD5|CD5 (p56-62)|epididymis secretory sperm binding protein|lymphocyte T1/Leu-1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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