Click image to see more details
Product Info Summary
| SKU: | A00387-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-IL-2/IL2 Antibody Picoband®
SKU/Catalog Number
A00387-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IL-2/IL2 Antibody Picoband® catalog # A00387-1. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IL-2/IL2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00387-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human IL-2 recombinant protein (Position: A21-T153). Human IL-2 shares 57.5% and 66.4% amino acid (aa) sequence identity with mouse and rat IL-2, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00387-1 is reactive to IL2 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
22 kDa
Calculated molecular weight
17628 MW
Background of IL-2
IL-2, Interleukin-2, formerly referred to as T-cell growth factor, is a powerfully immunoregulatory lymphokine that is produced by lectin-or antigen-activated T cells. In situ hybridization, the IL-2 gene is assigned to 4q26-q28. IL2 can act as a growth hormone for both B and T lymphocytes. It is useful in the study of the molecular nature of T-cell differentiation and, like interferons, augments natural killer cell activity.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00387-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA, 0.1-0.5μg/ml, -
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: HELA whole Cell, K562 whole Cell, RAJI whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of IL-2 using anti-IL-2 antibody (A00387-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: HELA whole Cell lysates,
Lane 2: K562 whole Cell lysates,
Lane 3: RAJI whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL-2 antigen affinity purified polyclonal antibody (Catalog # A00387-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL-2 at approximately 22KD. The expected band size for IL-2 is at 18KD.
Protein Target Info & Infographic
Gene/Protein Information For IL2 (Source: Uniprot.org, NCBI)
Gene Name
IL2
Full Name
Interleukin-2
Weight
17628 MW
Superfamily
IL-2 family
Alternative Names
Interleukin-2;IL-2;T-cell growth factor;TCGF;Aldesleukin;IL2; IL2 IL-2, TCGF, lymphokine interleukin 2 interleukin-2|T cell growth factor|aldesleukin|involved in regulation of T-cell clonal expansion
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IL2, check out the IL2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IL2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IL-2/IL2 Antibody Picoband® (A00387-1)
Hello CJ!
A00387-1 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Effects of recombinant hIL-2-Luffin P1 immunotoxin combining with arotinoid ethylester on the proliferation and apoptosis of Hut-78 cells
Women with Recurrent Miscarriage Have Decreased Expression of 25-Hydroxyvitamin D3-1?-Hydroxylase by the Fetal-Maternal Interface
Treatment of radiation-induced acute intestinal injury with bone marrow-derived mesenchymal stem cells
Gong T, Wang Cf, Yuan Jr, Li Y, Gu Jf, Zhao Bj, Zhang L, Jia Xb, Feng L, Liu Sl. Evid Based Complement Alternat Med. 2015;2015:630760. Doi: 10.1155/2015/630760. Epub 2015 May 3. Inhibition Of Tumor Growth And Immunomodulatory Effects Of Flavonoids...
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Customer Q&As
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16 Customer Q&As for Anti-IL-2/IL2 Antibody Picoband®
Question
My team were satisfied with the WB result of your anti-IL-2/IL2 antibody. However we have been able to see positive staining in blood secreted. using this antibody. Is that expected? Could you tell me where is IL2 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-03-13
Answer
From what I have seen in literature, blood does express IL2. Generally IL2 expresses in secreted. Regarding which tissues have IL2 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Placenta, Pubmed ID: 8824916
Boster Scientific Support
Answered: 2020-03-13
Question
See attached the WB image, lot number and protocol we used for small intestine peyer's patch using anti-IL-2/IL2 antibody A00387-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-17
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-17
Question
We need using your anti-IL-2/IL2 antibody for positive regulation of tyrosine phosphorylation of stat protein studies. Has this antibody been tested with western blotting on hela whole cell lysates? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-12-16
Answer
Thanks for your inquiry. This A00387-1 anti-IL-2/IL2 antibody is tested on hela whole cell lysates, k562 whole cell lysates. It is guaranteed to work for ELISA, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-12-16
Question
Can you help my question with product A00387-1, anti-IL-2/IL2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-06
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00387-1 anti-IL-2/IL2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-06
Question
We purchased anti-IL-2/IL2 antibody for ELISA on placenta in the past. I am using human, and I plan to use the antibody for WB next. I am interested in examining placenta as well as small intestine peyer's patch in our next experiment. Could give a recommendation on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-07-30
Answer
I looked at the website and datasheets of our anti-IL-2/IL2 antibody and it seems that A00387-1 has been validated on human in both ELISA and WB. Thus A00387-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-07-30
Question
We have been able to see staining in human small intestine peyer's patch. Do you have any suggestions? Is anti-IL-2/IL2 antibody supposed to stain small intestine peyer's patch positively?
Verified Customer
Verified customer
Asked: 2019-07-16
Answer
Based on literature small intestine peyer's patch does express IL2. Based on Uniprot.org, IL2 is expressed in small intestine peyer's patch, placenta, blood, among other tissues. Regarding which tissues have IL2 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Placenta, Pubmed ID: 8824916
Boster Scientific Support
Answered: 2019-07-16
Question
Will anti-IL-2/IL2 antibody A00387-1 work for WB with small intestine peyer's patch?
Verified Customer
Verified customer
Asked: 2019-06-07
Answer
According to the expression profile of small intestine peyer's patch, IL2 is highly expressed in small intestine peyer's patch. So, it is likely that anti-IL-2/IL2 antibody A00387-1 will work for WB with small intestine peyer's patch.
Boster Scientific Support
Answered: 2019-06-07
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for small intestine peyer's patch using anti-IL-2/IL2 antibody A00387-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-06-03
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-03
Question
My question regards to test anti-IL-2/IL2 antibody A00387-1 on human small intestine peyer's patch for research purposes, then I may be interested in using anti-IL-2/IL2 antibody A00387-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
N. Singh
Verified customer
Asked: 2019-04-19
Answer
The products we sell, including anti-IL-2/IL2 antibody A00387-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-04-19
Question
Is there a BSA free version of anti-IL-2/IL2 antibody A00387-1 available?
Verified Customer
Verified customer
Asked: 2018-07-10
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IL-2/IL2 antibody A00387-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-07-10
Question
Is this A00387-1 anti-IL-2/IL2 antibody reactive to the isotypes of IL2?
Verified Customer
Verified customer
Asked: 2018-07-03
Answer
The immunogen of A00387-1 anti-IL-2/IL2 antibody is E. coli-derived human IL-2 recombinant protein (Position: A21-T153). Human IL-2 shares 57.5% and 66.4% amino acid (aa) sequence identity with mouse and rat IL-2, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-07-03
Question
I was wanting to use your anti-IL-2/IL2 antibody for WB for human small intestine peyer's patch on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human small intestine peyer's patch identification?
Verified Customer
Verified customer
Asked: 2018-05-21
Answer
As indicated on the product datasheet, A00387-1 anti-IL-2/IL2 antibody has been validated for ELISA, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human small intestine peyer's patch in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-05-21
Question
We are currently using anti-IL-2/IL2 antibody A00387-1 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2018-04-24
Answer
The anti-IL-2/IL2 antibody (A00387-1) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-04-24
Question
Would A00387-1 anti-IL-2/IL2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-07-04
Answer
As indicated on the product datasheet, A00387-1 anti-IL-2/IL2 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-07-04
Question
I see that the anti-IL-2/IL2 antibody A00387-1 works with WB, what is the protocol used to produce the result images on the product page?
B. Carter
Verified customer
Asked: 2016-08-31
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2016-08-31
Question
Is a blocking peptide available for product anti-IL-2/IL2 antibody (A00387-1)?
C. Singh
Verified customer
Asked: 2015-08-20
Answer
We do provide the blocking peptide for product anti-IL-2/IL2 antibody (A00387-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2015-08-20
