Click image to see more details
Product Info Summary
| SKU: | M00480-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CD5 Monoclonal Antibody
SKU/Catalog Number
M00480-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CD5 Monoclonal Antibody catalog # M00480-1. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD5 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00480-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ACII-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CD5 May act as a receptor in regulating T-cell proliferation. CD5 interacts with CD72/LYB-2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00480-1 is reactive to CD5 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
501 kDa
Calculated molecular weight
86045 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00480-1 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:5000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
Positive Control
WB: human plasma
Validation Images & Assay Conditions
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Western blot analysis of CD5 expression in human plasma lysate.
Protein Target Info & Infographic
Gene/Protein Information For CD5 (Source: Uniprot.org, NCBI)
Gene Name
CD5
Full Name
T-cell surface glycoprotein CD5
Weight
86045 MW
Alternative Names
Microprocessor complex subunit DGCR8;DiGeorge syndrome critical region 8;DGCR8;C22orf12, DGCRK6;LP4941; CD5 LEU1, T1 CD5 molecule T-cell surface glycoprotein CD5|CD5 (p56-62)|epididymis secretory sperm binding protein|lymphocyte T1/Leu-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD5, check out the CD5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD5 Monoclonal Antibody (M00480-1)
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No publications found for M00480-1
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Recommended Resources
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-CD5 Monoclonal Antibody
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-CD5 Monoclonal antibody M00480-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-04-01
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-01
Question
Will anti-CD5 Monoclonal antibody M00480-1 work for IF with leukemic t-cell?
Verified Customer
Verified customer
Asked: 2020-03-12
Answer
According to the expression profile of leukemic t-cell, CD5 is highly expressed in leukemic t-cell. So, it is likely that anti-CD5 Monoclonal antibody M00480-1 will work for IF with leukemic t-cell.
Boster Scientific Support
Answered: 2020-03-12
Question
I am looking for to test anti-CD5 Monoclonal antibody M00480-1 on human leukemic t-cell for research purposes, then I may be interested in using anti-CD5 Monoclonal antibody M00480-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-10-09
Answer
The products we sell, including anti-CD5 Monoclonal antibody M00480-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-09
Question
See below the WB image, lot number and protocol we used for leukemic t-cell using anti-CD5 Monoclonal antibody M00480-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-09-30
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-30
Question
We are currently using anti-CD5 Monoclonal antibody M00480-1 for human tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-30
Answer
The anti-CD5 Monoclonal antibody (M00480-1) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-30
Question
I was wanting to use your anti-CD5 Monoclonal antibody for IF for human leukemic t-cell on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human leukemic t-cell identification?
Verified Customer
Verified customer
Asked: 2019-05-24
Answer
As indicated on the product datasheet, M00480-1 anti-CD5 Monoclonal antibody has been validated for IP, IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human leukemic t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-24
Question
We ordered your anti-CD5 Monoclonal antibody for IF on pancreas in the past. I am using human, and I plan to use the antibody for WB next. I am interested in examining pancreas as well as thymus in our next experiment. Could give a recommendation on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-02-21
Answer
I looked at the website and datasheets of our anti-CD5 Monoclonal antibody and it seems that M00480-1 has been tested on human in both IF and WB. Thus M00480-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-02-21
Question
My question regarding product M00480-1, anti-CD5 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
M. Miller
Verified customer
Asked: 2018-12-25
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00480-1 anti-CD5 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-12-25
Question
Is there a BSA free version of anti-CD5 Monoclonal antibody M00480-1 available?
Verified Customer
Verified customer
Asked: 2018-02-16
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD5 Monoclonal antibody M00480-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-02-16
Question
Our team were satisfied with the WB result of your anti-CD5 Monoclonal antibody. However we have observed positive staining in leukemic t-cell cell membrane using this antibody. Is that expected? Could you tell me where is CD5 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-01-09
Answer
From literature, leukemic t-cell does express CD5. Generally CD5 expresses in cell membrane. Regarding which tissues have CD5 expression, here are a few articles citing expression in various tissues:
Leukemic T-cell, Pubmed ID: 15144186, 19690332
Lymphocyte, Pubmed ID: 8740779
Pancreas, Pubmed ID: 15489334
Thymus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2018-01-09
Question
Does M00480-1 anti-CD5 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
R. Thomas
Verified customer
Asked: 2014-07-16
Answer
It shows on the product datasheet, M00480-1 anti-CD5 Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-07-16
Question
Is this M00480-1 anti-CD5 Monoclonal antibody reactive to the isotypes of CD5?
N. Anderson
Verified customer
Asked: 2014-07-15
Answer
The immunogen of M00480-1 anti-CD5 Monoclonal antibody is A synthesized peptide derived from human CD5 May act as a receptor in regulating T-cell proliferation. CD5 interacts with CD72/LYB-2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-07-15
Question
I see that the anti-CD5 Monoclonal antibody M00480-1 works with IF, what is the protocol used to produce the result images on the product page?
D. Yang
Verified customer
Asked: 2014-04-23
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2014-04-23
Question
Is a blocking peptide available for product anti-CD5 Monoclonal antibody (M00480-1)?
B. Jackson
Verified customer
Asked: 2013-09-19
Answer
We do provide the blocking peptide for product anti-CD5 Monoclonal antibody (M00480-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2013-09-19
Question
We have observed staining in human leukemic t-cell. Any tips? Is anti-CD5 Monoclonal antibody supposed to stain leukemic t-cell positively?
V. Krishna
Verified customer
Asked: 2013-09-02
Answer
According to literature leukemic t-cell does express CD5. According to Uniprot.org, CD5 is expressed in leukocyte, lymphocyte, thymus, pancreas, leukemic t-cell, among other tissues. Regarding which tissues have CD5 expression, here are a few articles citing expression in various tissues:
Leukemic T-cell, Pubmed ID: 15144186, 19690332
Lymphocyte, Pubmed ID: 8740779
Pancreas, Pubmed ID: 15489334
Thymus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2013-09-02
