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Product Info Summary
| SKU: | PB9333 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-CD44 Antibody Picoband®
SKU/Catalog Number
PB9333
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD44 Antibody Picoband® catalog # PB9333. Tested in IF, IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD44 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9333)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human CD44, different from the related mouse and rat sequences by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9333 is reactive to CD44 in Human, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
82 kDa
Calculated molecular weight
81538 MW
Background of CD44
CD44 is also known as LHR or MC56. The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9333 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Immunofluorescence, 2μg/ml, Human, Rat
Positive Control
WB: human Hela whole cell, human U87 whole cell, rat PC-12 whole cell, rat C6 whole cell
IHC: Human Intestinal Cancer tissue
IF: human tonsil tissue, rat lymphaden tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of CD44 using anti-CD44 antibody (PB9333).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human U87 whole cell lysates,
Lane 3: rat PC-12 whole cell lysates,
Lane 4: rat C6 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD44 antigen affinity purified polyclonal antibody (Catalog # PB9333) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD44 at approximately 82 kDa. The expected band size for CD44 is at 82 kDa.
Click image to see more details
Figure 2. IHC analysis of CD44 using anti-CD44 antibody (PB9333).
CD44 was detected in a paraffin-embedded section of Human Intestinal Cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CD44 Antibody (PB9333) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. IF analysis of CD44 using anti-CD44 antibody (PB9333)
CD44 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-CD44 Antibody (PB9333) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 4. IF analysis of CD44 using anti-CD44 antibody (PB9333)
CD44 was detected in paraffin-embedded section of rat lymphaden tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-CD44 Antibody (PB9333) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 5. Western blot analysis of CD44 using anti-CD44 antibody (PB9333).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% milk in PBS/0.05% Tween-20 (5% milk/PBS/Tw) for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD44 antibody ( PB9333) at 1 ug/mL in 5% milk/PBS/0.05% Tween 20 overnight at 4℃ , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit antibody conjugated with HRP at 1:5,000 in 5% milk/PBS/Tw at 4℃ for 12 hours. The signal is developed using an SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific). A specific band was detected for EGFR at approximately 20 kDa. The expected band size for EGFR is at 81 kDa.
Protein Target Info & Infographic
Gene/Protein Information For CD44 (Source: Uniprot.org, NCBI)
Gene Name
CD44
Full Name
CD44 antigen
Weight
81538 MW
Alternative Names
CD44 antigen;CDw44;Epican;Extracellular matrix receptor III;ECMR-III;GP90 lymphocyte homing/adhesion receptor;HUTCH-I;Heparan sulfate proteoglycan;Hermes antigen;Hyaluronate receptor;Phagocytic glycoprotein 1;PGP-1;Phagocytic glycoprotein I;PGP-I;CD44;CD44;LHR, MDU2, MDU3, MIC4; CD44 CDW44, CSPG8, ECMR-III, HCELL, HUTCH-I, IN, LHR, MC56, MDU2, MDU3, MIC4, Pgp1 CD44 molecule (Indian blood group) CD44 |GP90 lymphocyte homing/adhesion receptor|Hermes |Indian blood group |cell surface glycoprotein CD44|chondroitin sulfate proteoglycan 8|epican|extracellular matrix receptor III|hematopoietic cell E- and L-selectin ligand|heparan sulfate proteoglycan|homing function and Indian blood group system|hyaluronate receptor|phagocytic glycoprotein 1|soluble CD44
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD44, check out the CD44 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD44: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD44 Antibody Picoband® (PB9333)
Hello CJ!
PB9333 has been cited in 37 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Identification and differentiation therapy strategy of pterygium in vitro
Smad3-related miRNAs regulated oncogenic TRIB2 promoter activity to effectively suppress lung adenocarcinoma growth
Ternary Nanoparticles with a Sheddable Shell Efficiently Deliver MicroRNA-34a against CD44-Positive Melanoma
An improved osseointegration of metal implants by pitavastatin loaded multilayer films with osteogenic and angiogenic properties
HER2 Interacts With CD44 to Up-regulate CXCR4 via Epigenetic Silencing of microRNA-139 in Gastric Cancer Cells
Species: Human
Kubatka P,Kello M,Kajo K,Samec M,Jasek K,Vybohova D,Uramova S,Liskova A,Sadlonova V,Koklesova L,Murin R,Adamkov M,Smejkal K,Svajdlenka E,Solar P,Samuel SM,Kassayova M,Kwon TK,Zubor P,Pec M,Danko J,Büsselberg D,Mojzis J.Chemopreventive and Therapeutic Efficacy of Cinnamomum zeylanicum L. Bark in Experimental Breast Carcinoma: Mechanistic In Vivo and In Vitro Analyses.Molecules.2020 Mar 19;25(6):1399.doi:10.3390/molecules25061399.PMID:32204409;PMCID:PMC7144360.
Species: Mouse,Rat
PB9333 usage in article: APP:IHC, SAMPLE:MAMMARY GLAND TISSUE, DILUTION:NA
Wu,H.,Jiang,X.,Li,Y.,Zhou,Y.,Zhang,T.,Zhi,P.,Gao,J.,Engineering Stem Cell Derived Biomimetic Vesicles for Versatility and Effective Targeted Delivery.Adv. Funct.Mater.2020, 30, 2006169.https://doi.org/10.1002/adfm.202006169
Species: Rat,Mouse
PB9333 usage in article: APP:WB, SAMPLE:MSC, DILUTION:NA
ACS Appl.Bio Mater.2020,XXXX,XXX,XXX-XXX Publication Date:December 15, 2020 https://doi.org/10.1021/acsabm.0c00927
Species: Human,Mouse
PB9333 usage in article: APP:IF, SAMPLE:4T1 CELL AND HUVECS, DILUTION:1:200
Zhang GF,Wu JC,Wang HY,Jiang WD,Qiu L. Overexpression of microRNA-205-5p exerts suppressive effects on stem cell drug resistance in gallbladder cancer by down-regulating PRKCE. Biosci Rep.2020 Sep 30;40(9): BSR20194509.doi:10.1042/BSR20194509.PMID: 328698
Species: Human,Mouse
PB9333 usage in article: APP:FLOW CYTOMETRY, SAMPLE:GBC STEM CELLS, DILUTION:NA
Kubatka P,Kello M,Kajo K,Samec M,Liskova A,Jasek K,Koklesova L,Kuruc T,Adamkov M,Smejkal K,Svajdlenka E,Solar P,Pec M,Büsselberg D,Sadlonova V,Mojzis J.Rhus coriaria L.(Sumac) Demonstrates Oncostatic Activity in the Therapeutic and Preventive Model of Bre
Species: Human,Mouse,Rat
PB9333 usage in article: APP:IHC, SAMPLE:MAMMARY TISSUE, DILUTION:NA
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Customer Reviews
Have you used Anti-CD44 Antibody Picoband®?
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2 Reviews For Anti-CD44 Antibody Picoband®
0
Good enough for publishing--Chun-Chih Tseng, NORRIS CANCER CENTER, UNIVERSITY OF SOUTHERN CALIFORNIA, RESEARCH ASSISTANT
Excellent
Source: Biocompare.com
| Applications | Western Blot |
|---|---|
| Sample | Human breast cancer cell line |
| Detection | Bio-Rad imager |
"I used this anti-CD44 C-terminus antibody to detect cleaved/non-cleaved CD44 product. The results for Western blot are okay. The signal (1:1500) is not super strong but good enough to see clear bands. This antibody could detect a band the same size as another antibody that I used against the extracellular region of CD44, indicating this antibody could detect CD44. CD44 has multiple spliced isoforms. The WB result showed multiple bands, some of the bands I am interested in overlapped with the bands generated by another CD44 antibody targeting the extracellular domains. So, the specificity of this antibody is probably okay. The intensity of bands are acceptable, good enough for publishing. Relatively inexpnsive, okay specificity. Good enough to detect CD44 C-term."
Chun-Chih Tseng
Verified customer
Submitted 2016-08-24
0
High specificity and stability--Elena Deryugina, CELL AND MOLECULAR BIOLOGY, THE SCRIPPS RESEARCH INSTITUTE, ASSISTANT PROFESSOR
Excellent
Source: Biocompare.com
| Applications | Western Blot |
|---|---|
| Sample | Tumor cell lysate and recombinant protein |
| Detection | SuperSignal West Pico Chemiluminescent Substrate |
"The rabbit antibody PB9333 from Boster produced very good results in western blotting with the lysate of human CD44-positive cancer cells as well as with the recombinant N-terminal protein. The detection was specific, resulting only in the protein bands of expected mol. weights. The antibody is also capable of detecting shed CD44 in conditioned medium. High specificity and stability. I would highly recommend this antibody for detection of CD44 in human cells by western blotting."
Elena Deryugina
Verified customer
Submitted 2016-08-24
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
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Submit your question
16 Customer Q&As for Anti-CD44 Antibody Picoband®
Question
I am looking for using your anti-CD44 antibody for positive regulation of peptidyl-serine phosphorylation studies. Has this antibody been tested with western blotting on intestinal cancer tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-03-31
Answer
We appreciate your inquiry. This PB9333 anti-CD44 antibody is validated on hela whole cell lysate, panc whole cell lysate, intestinal cancer tissue. It is guaranteed to work for IF, IHC-P, WB in human, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-03-31
Question
I am interested in to test anti-CD44 antibody PB9333 on human keratinocyte for research purposes, then I may be interested in using anti-CD44 antibody PB9333 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-02-04
Answer
The products we sell, including anti-CD44 antibody PB9333, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-02-04
Question
Is this PB9333 anti-CD44 antibody reactive to the isotypes of CD44?
Verified Customer
Verified customer
Asked: 2019-12-18
Answer
The immunogen of PB9333 anti-CD44 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human CD44(29-56aa RFAGVFHVEKNGRYSISRTEAADLCKAF), different from the related mouse and rat sequences by two amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-12-18
Question
We purchased anti-CD44 antibody for IF on reticulocyte in a previous project. I am using rat, and I plan to use the antibody for IHC-P next. We need examining reticulocyte as well as glial tumor in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC-P?
Verified Customer
Verified customer
Asked: 2019-10-15
Answer
I took a look at the website and datasheets of our anti-CD44 antibody and it appears that PB9333 has been tested on rat in both IF and IHC-P. Thus PB9333 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC-P in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC-P detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-10-15
Question
Would anti-CD44 antibody PB9333 work for IF with keratinocyte?
Verified Customer
Verified customer
Asked: 2019-09-02
Answer
According to the expression profile of keratinocyte, CD44 is highly expressed in keratinocyte. So, it is likely that anti-CD44 antibody PB9333 will work for IF with keratinocyte.
Boster Scientific Support
Answered: 2019-09-02
Question
Is a blocking peptide available for product anti-CD44 antibody (PB9333)?
Verified Customer
Verified customer
Asked: 2019-08-07
Answer
We do provide the blocking peptide for product anti-CD44 antibody (PB9333). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-08-07
Question
I was wanting to use your anti-CD44 antibody for IF for human keratinocyte on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human keratinocyte identification?
Verified Customer
Verified customer
Asked: 2019-07-12
Answer
As indicated on the product datasheet, PB9333 anti-CD44 antibody has been tested for IF, IHC-P, WB on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in human keratinocyte in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-12
Question
I see that the anti-CD44 antibody PB9333 works with IF, what is the protocol used to produce the result images on the product page?
M. Collins
Verified customer
Asked: 2018-10-31
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-10-31
Question
We have seen staining in rat articular cartilage. Any tips? Is anti-CD44 antibody supposed to stain articular cartilage positively?
Verified Customer
Verified customer
Asked: 2018-06-25
Answer
Based on literature articular cartilage does express CD44. Based on Uniprot.org, CD44 is expressed in parotid gland, reticulocyte, myeloid leukemia cell, keratinocyte, lymphoblast, mammary carcinoma, articular cartilage, colon adenocarcinoma retinal pigment epithelium, spinal cord, pancreas retinal pigment epithelium, glial tumor, peripheral blood, foreskin, lung, plasma, cervix carcinoma, t-cell, liver, leukemic t-cell, among other tissues. Regarding which tissues have CD44 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 18691976, 20068231, 23186163
Foreskin, Pubmed ID: 2007624
Glial tumor, Pubmed ID: 7527301
Keratinocyte, Pubmed ID: 1281868
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 19159218, 24275569
Lung, Pubmed ID: 1717145, 7528188
Lymphoblast, Pubmed ID: 1465456, 1922057
Mammary carcinoma, Pubmed ID: 8352881
Myeloid leukemia cell, Pubmed ID: 2056274
Pancreas, and Retinal pigment epithelium, Pubmed ID: 15489334
Peripheral blood, Pubmed ID: 7508992
Plasma, Pubmed ID: 16335952
Reticulocyte, Pubmed ID: 1840487
Spinal cord, Pubmed ID: 17974005
T-cell, Pubmed ID: 19367720
Boster Scientific Support
Answered: 2018-06-25
Question
We are currently using anti-CD44 antibody PB9333 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, rat. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2018-04-19
Answer
The anti-CD44 antibody (PB9333) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-04-19
Question
Our lab were content with the WB result of your anti-CD44 antibody. However we have observed positive staining in spinal cord cell membrane using this antibody. Is that expected? Could you tell me where is CD44 supposed to be expressed?
T. Miller
Verified customer
Asked: 2017-02-06
Answer
According to literature, spinal cord does express CD44. Generally CD44 expresses in cell membrane. Regarding which tissues have CD44 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 18691976, 20068231, 23186163
Foreskin, Pubmed ID: 2007624
Glial tumor, Pubmed ID: 7527301
Keratinocyte, Pubmed ID: 1281868
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 19159218, 24275569
Lung, Pubmed ID: 1717145, 7528188
Lymphoblast, Pubmed ID: 1465456, 1922057
Mammary carcinoma, Pubmed ID: 8352881
Myeloid leukemia cell, Pubmed ID: 2056274
Pancreas, and Retinal pigment epithelium, Pubmed ID: 15489334
Peripheral blood, Pubmed ID: 7508992
Plasma, Pubmed ID: 16335952
Reticulocyte, Pubmed ID: 1840487
Spinal cord, Pubmed ID: 17974005
T-cell, Pubmed ID: 19367720
Boster Scientific Support
Answered: 2017-02-06
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for keratinocyte using anti-CD44 antibody PB9333. Let me know if you need anything else.
D. Wu
Verified customer
Asked: 2016-09-12
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-09-12
Question
I have a question about product PB9333, anti-CD44 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
P. Krishna
Verified customer
Asked: 2015-08-31
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9333 anti-CD44 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-08-31
Question
Here is the WB image, lot number and protocol we used for keratinocyte using anti-CD44 antibody PB9333. Please let me know if you require anything else.
J. Singh
Verified customer
Asked: 2015-04-14
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-04-14
Question
Do you have a BSA free version of anti-CD44 antibody PB9333 available?
C. Wu
Verified customer
Asked: 2014-12-24
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD44 antibody PB9333 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2014-12-24
Question
Will PB9333 anti-CD44 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
V. Kulkarni
Verified customer
Asked: 2013-12-17
Answer
As indicated on the product datasheet, PB9333 anti-CD44 antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-12-17
