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Product Info Summary
| SKU: | M01508 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, WB |
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Product info
Product Name
Anti-CD33 Monoclonal Antibody
View all Siglec-3/CD33 Antibodies
SKU/Catalog Number
M01508
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CD33 Monoclonal Antibody catalog # M01508. Tested in WB, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD33 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01508)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ADGE-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CD33 Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01508 is reactive to CD33 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
43 kDa
Calculated molecular weight
39330 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01508 is guaranteed for IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IP 1:50
Positive Control
WB: THP1 cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of CD33 expression in THP1 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For CD33 (Source: Uniprot.org, NCBI)
Gene Name
CD33
Full Name
Myeloid cell surface antigen CD33
Weight
39330 MW
Superfamily
immunoglobulin superfamily
Alternative Names
Porphobilinogen deaminase;PBG-D;2.5.1.61;Hydroxymethylbilane synthase;HMBS;Pre-uroporphyrinogen synthase;HMBS;PBGD, UPS; CD33 SIGLEC-3, SIGLEC3, p67 CD33 molecule myeloid cell surface CD33|CD33 (gp67)|CD33 molecule transcript|gp67|sialic acid-binding Ig-like lectin 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD33, check out the CD33 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD33: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD33 Monoclonal Antibody (M01508)
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No publications found for M01508
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Recommended Resources
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-CD33 Monoclonal Antibody
Question
I was wanting to use your anti-CD33 Monoclonal antibody for IP for human premonocytic lymphoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human premonocytic lymphoma identification?
Verified Customer
Verified customer
Asked: 2020-04-29
Answer
You can see on the product datasheet, M01508 anti-CD33 Monoclonal antibody has been validated for IP, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human premonocytic lymphoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-29
Question
Does anti-CD33 Monoclonal antibody M01508 work for IP with premonocytic lymphoma?
Verified Customer
Verified customer
Asked: 2020-04-27
Answer
According to the expression profile of premonocytic lymphoma, CD33 is highly expressed in premonocytic lymphoma. So, it is likely that anti-CD33 Monoclonal antibody M01508 will work for IP with premonocytic lymphoma.
Boster Scientific Support
Answered: 2020-04-27
Question
We have observed staining in human uterus. Are there any suggestions? Is anti-CD33 Monoclonal antibody supposed to stain uterus positively?
N. Brown
Verified customer
Asked: 2020-04-15
Answer
From literature uterus does express CD33. From Uniprot.org, CD33 is expressed in leukocyte, premonocytic lymphoma, uterus, among other tissues. Regarding which tissues have CD33 expression, here are a few articles citing expression in various tissues:
Leukocyte, Pubmed ID: 15489334
Premonocytic lymphoma, Pubmed ID: 3139766, 11943481
Uterus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2020-04-15
Question
Our team were happy with the WB result of your anti-CD33 Monoclonal antibody. However we have seen positive staining in premonocytic lymphoma isoform cd33m: peroxisome using this antibody. Is that expected? Could you tell me where is CD33 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-01-15
Answer
From literature, premonocytic lymphoma does express CD33. Generally CD33 expresses in isoform cd33m: cell membrane, isoform cd33m: peroxisome. Regarding which tissues have CD33 expression, here are a few articles citing expression in various tissues:
Leukocyte, Pubmed ID: 15489334
Premonocytic lymphoma, Pubmed ID: 3139766, 11943481
Uterus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2020-01-15
Question
We are currently using anti-CD33 Monoclonal antibody M01508 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-03
Answer
The anti-CD33 Monoclonal antibody (M01508) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-03
Question
What is the sample target on Western blot analysis in your product image for M01508?
Verified customer
Asked: 2019-12-09
Answer
Western blot analysis of the Anti-CD33 Monoclonal Antibody (M01508) expression in THP1 cell lysate.
Boster Scientific Support
Answered: 2019-12-10
Question
Is there a BSA free version of anti-CD33 Monoclonal antibody M01508 available?
Verified Customer
Verified customer
Asked: 2019-12-02
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-CD33 Monoclonal antibody M01508 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-02
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for premonocytic lymphoma using anti-CD33 Monoclonal antibody M01508. Let me know if you need anything else.
L. Anderson
Verified customer
Asked: 2019-10-07
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-07
Question
See attached the WB image, lot number and protocol we used for premonocytic lymphoma using anti-CD33 Monoclonal antibody M01508. Please let me know if you require anything else.
B. Johnson
Verified customer
Asked: 2019-09-12
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-12
Question
I have a question about product M01508, anti-CD33 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-05-30
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01508 anti-CD33 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-30
Question
Is this M01508 anti-CD33 Monoclonal antibody reactive to the isotypes of CD33?
Verified Customer
Verified customer
Asked: 2018-08-06
Answer
The immunogen of M01508 anti-CD33 Monoclonal antibody is A synthesized peptide derived from human CD33 Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-08-06
Question
Is a blocking peptide available for product anti-CD33 Monoclonal antibody (M01508)?
Verified Customer
Verified customer
Asked: 2017-09-28
Answer
We do provide the blocking peptide for product anti-CD33 Monoclonal antibody (M01508). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-09-28
Question
I see that the anti-CD33 Monoclonal antibody M01508 works with IP, what is the protocol used to produce the result images on the product page?
K. Jackson
Verified customer
Asked: 2016-10-17
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2016-10-17
Question
Our lab used your anti-CD33 Monoclonal antibody for IP on leukocyte a few months ago. I am using human, and I plan to use the antibody for WB next. I am looking for examining leukocyte as well as uterus in our next experiment. Do you have any suggestion on which antibody would work the best for WB?
S. Dhar
Verified customer
Asked: 2016-03-21
Answer
I looked at the website and datasheets of our anti-CD33 Monoclonal antibody and it seems that M01508 has been validated on human in both IP and WB. Thus M01508 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2016-03-21
Question
I was wanting to use to test anti-CD33 Monoclonal antibody M01508 on human premonocytic lymphoma for research purposes, then I may be interested in using anti-CD33 Monoclonal antibody M01508 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
T. Anderson
Verified customer
Asked: 2016-02-03
Answer
The products we sell, including anti-CD33 Monoclonal antibody M01508, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-02-03
Question
Does M01508 anti-CD33 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
G. Taylor
Verified customer
Asked: 2013-09-19
Answer
It shows on the product datasheet, M01508 anti-CD33 Monoclonal antibody as been tested on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-09-19
